Lactobacillus plantarum in Dual-Species Biofilms With Listeria monocytogenes Enhanced the Anti-Listeria Activity of a Commercial Disinfectant Based on Hydrogen Peroxide and Peracetic Acid

Abstract

The aim of this work was to investigate the effect of dual-species biofilms of Listeria monocytogenes with Lactobacillus plantarum on the anti-Listeria activity of a hydrogen peroxide/peracetic acid based commercial disinfectant (P3, Oxonia) when using conditions approaching the food industry environment. Nine strains of L. monocytogenes, including eight persistent strains collected from the meat industry and one laboratory control strain, were used in mono and in dual-species biofilms with a strain of L. plantarum. Biofilms were produced on stainless steel coupons (SSCs), at 11°C (low temperature) or at 25°C (control temperature), in TSB-YE (control rich medium) or in 1/10 diluted TSB-YE (mimicking the situation of biofilm formation after a deficient industrial cleaning procedure). The biofilm forming ability of the strains was evaluated by enumeration of viable cells, and the antibiofilm activity of P3 was assessed by the log reduction of viable cells on SSC. In both nutrient conditions and at low temperature, there was no significant difference (p > 0.05) between L. monocytogenes biofilm forming ability in mono- and in dual-species biofilms. In dual-species biofilms, L. monocytogenes was the dominant species. However, it was generally more susceptible to the lower concentration of P3 0.5% (v/v) than in pure culture biofilms. The presence of L. plantarum, although without significant interference in the number of viable cells of L. monocytogenes, enhanced the efficacy of the anti-Listeria activity of P3, since dual-species biofilms were easier to control. The results presented here reinforce the importance of the investigation into co-culture biofilms produced in food industry conditions, namely at low temperatures, when susceptibility to sanitizers is being assessed.

Use of probiotic lactobacilli in the treatment of vaginal infections: In vitro and in vivo investigations

“…H 2 O 2 is an oxidizing chemical that is hazardous to catalase-negative bacteria, including most anaerobes. It exhibits significant antimicrobial action in vitro , and colonization with lactobacilli that generate H 2 O 2 has been linked to a decreased incidence of BV, preterm delivery, and HIV infection ( Bonneville et al., 2021 ). Bacteriocins are a category of potent, ribosomally produced peptides that are active against Gram-positive and Gram-negative bacteria, as well as some fungi ( Mokoena et al., 2021 ; Darbandi et al., 2022 ).…”

Biofilms as a microbial hazard in the food industry: A scoping review

“…Moreover, the multi‐species biofilms were found to have higher resistance to disinfectants such as quaternary ammonium compounds (QAC) and other biocides (Meyer, 2003). In a study by Bonneville et al (2021), P3's disinfectant (active compound: hydrogen peroxide and peracetic acid) anti‐Listeria activity was increased by L. plantarum in dual‐species biofilms formed with L. monocytogenes possibly due to release of hydrolase enzyme which was found in the exoproteome of L. plantarum and might have interfered with the biofilm matrix's stability, making it susceptible towards the disinfectant. Although L. plantarum had no impact on the number of viable L. monocytogenes cells in the biofilm but it did make it more reactive to P3 disinfectant exposure.…”

Prospects for the use of probiotic organisms to develop alternative strategies for disinfection and prevention of infectious diseases

“…Метод включал прямое нанесение бактериоциногенного штамма, бактериоцинов или их биоинженерных препаратов на биопленки Listeria monocytogenes. Авторы отмечали, что при использовании штамма, возможно, ингибирующий эффект принадлежал не только бактериоцинам, но и другим антимикробным веществам (Bonneville et al, 2021;Guerrieri et al, 2009). Так есть данные о высокой эффективности против старых биопленок средства на основе молочной кислоты (Cui et al, 2018;Zeng et al, 2022) (Hossain et al, 2018;Laleman et al, 2014).…”

“…При режиме обработки 4 раза в неделю полное уничтожение биопленки Listeria monocytogenes было достигнуто за 8 недель. Авторы предлагают регулярную профилактическую обработку с помощью молочнокислых бактерий (МКБ) в качестве простой и экологически безопасной стратегии борьбы с биопленками(Bonneville et al, 2021). Сообщалось также о том, что не-Lactobacillus salivarius ингибируют рост Streptococcus mutans in vitro и in vivo…”

“…С. А. Кишилова ферменты, возможно, нарушали целостность матрицы биопленки, делая ее более уязвимой к действию дезинфицирующих средств(Bonneville, 2021).Enterococcus durans 152 и которые МКБ, полученные из кимчи (ферментированных овощей), оказывают ингибирующее действие против биопленок Listeria monocytogenes на поверхностях из нержавеющей стали(Tan et al, 2017).…”