Lactobacillus plantarum Inhibits the Intestinal Epithelial Migration of Neutrophils Induced by Enteropathogenic Escherichia coli

Michail, Sonia; Abernathy, Frank

doi:10.1097/00005176-200303000-00017

Cited by 40 publications

(19 citation statements)

References 24 publications

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“…Dead cells of probiotics exert a number of biological responses during in vitro cell-culture and in vivo animal studies (13). However, several of the effects exerted by live strains on IECs are notably absent with dead cells, such as maintaining barrier function after an enteropathogen insult and colonization resistance after enteropathogen adherence (14,15). Dead cells also fail to prevent against cytokineinduced apoptosis (16) and have shown to have inferior efficacy in clinical trials in reducing translocation (17) compared with live, viable probiotics.…”

Section: Discussionmentioning

confidence: 99%

Pulse Probiotic Administration Induces Repeated Small Intestinal Muc3 Expression in Rats

et al. 2011

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Upon ingestion, probiotics may act to protect the host through a number of protective mechanisms including modulation of genes involved in intestinal innate mucosal defense such as epithelial cell-derived mucin glycoproteins and inhibitor of apoptosis proteins. To determine the specificity of effect and sustainability of response in vivo, Lactobacillus plantarum 299v (Lp299v), Lactobacillus rhamnosus R0011 (LrR0011), and Bifidobacterium bifidum R0071 (BbR0071) were added repeatedly or intermittently to the drinking water of Sprague-Dawley rats. After killing the rats via CO 2 suffocation, Muc2, Muc3, neuronal apoptosis inhibitor protein (NAIP), human inhibitor of apoptosis protein 1/cellular inhibitor of apoptosis 2 (HIAP1/cIAP2), and human inhibitor of apoptosis protein 2/cellular inhibitor of apoptosis 1 (HIAP2/cIAP1) mRNA and protein levels were analyzed via RT-PCR and immunohistochemistry. Live Lp299v, BbR0071, and LrR0011 increased Muc3 protein and mRNA expression in jejunum and ileum. Heat-killed and a nonadherent derivative of Lp299v failed to induce Muc3 expression. Lp299v did induce expression of HIAP2/cIAP1 and NAIP expression. Muc3 mucin expression was elevated for 5 d after oral administration of Lp299v; however, this effect was not sustained despite ongoing daily ingestion of a probiotic. Intermittent pulse ingestion of probiotics, however, was found to repeatedly increase Muc3 expression. We conclude that selected probiotics can induce protective genes of mucosal intestinal epithelial cells, an effect that is reproducible with pulse probiotic administration. (Pediatr Res 69: 206-211, 2011) P robiotics are living organisms that improve the health of the host upon ingestion (1). One mechanism whereby probiotics exert their influence is through enhancement of intestinal barrier function (1). The intestinal mucosal barrier consists of both the intestinal epithelial cell (IEC) and the supramucosal mucus layer whose major organic component is intestinal cell-derived mucins. Mucins are complex glycoproteins that act to limit access to IECs by both by creating a physicochemical barrier and through enteropathogen IEC receptor mimicry (2,3).Probiotics have a multitude of effects in germ-free animals and sterile cell culture system. However, to determine whether mucosal protection can be enhanced exogenously by probiotics, use of specific pathogen-free rats offers a method to study probiotic administration into an established microbial environment.In cell cultures, selected probiotic strains attenuate the stress-induced death of IECs. IEC homeostasis is maintained in the gastrointestinal tract mainly via apoptosis, via important regulators of apoptosis including members of the inhibitor of apoptosis protein (IAP) family (4). IAPs have a number of other roles including regulating proliferation and modulation of inflammatory signaling and immunity (5). IECs that are lost through a physiologic process are replenished at an equally rapid rate in villi crypts, on average every 3-5 d (4,6). With some of t...

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Section: Discussionmentioning

confidence: 99%

Pulse Probiotic Administration Induces Repeated Small Intestinal Muc3 Expression in Rats

et al. 2011

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“…In contrast to the untreated group, E. coli did not negatively influence the permeability of the intestinal cell wall of rats that received L. plantarum (Mangell et al, 2002). Pre-incubation of an intestinal epithelial T-84 monolayer with L. plantarum, showed a reduced attachment of enteropathogenic E. coli and reduction in inflammatory factors, like neutrophil migration (Michail & Abernathy, 2003). However, short-term addition of L. plantarum did not reduce the effect of E. coli in similar experiments on the intestinal cells (Mangell et al, 2002;Michail & Abernathy, 2003).…”

Section: Effects Of L Plantarum On Diseasementioning

confidence: 90%

“…Pre-incubation of an intestinal epithelial T-84 monolayer with L. plantarum, showed a reduced attachment of enteropathogenic E. coli and reduction in inflammatory factors, like neutrophil migration (Michail & Abernathy, 2003). However, short-term addition of L. plantarum did not reduce the effect of E. coli in similar experiments on the intestinal cells (Mangell et al, 2002;Michail & Abernathy, 2003). A possible mechanism is competitive exclusion between L. plantarum and E. coli via the mannose adhesin that can be involved in competition for the mannose-specific binding sites as described earlier (Pretzer et al, 2004).…”

Section: Effects Of L Plantarum On Diseasementioning

confidence: 99%

Lactobacillus plantarum—survival, functional and potential probiotic properties in the human intestinal tract

Vries

Vaughan

Kleerebezem

et al. 2006

International Dairy Journal

435

279

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“…They can exert some of their effects at the mucosal-luminal interface through their actions on intestinal epithelial cells (IECs). Select probiotics can upregulate IEC mucin expression to impede enteropathogen access to IECs [1]; maintain barrier function following enteropathogen insult and reduce enteropathogen colonization and adherence [2,3]; modulate Toll-like receptor 2, critical in maintaining barrier function when cells are faced with inflammatory stress [4]; and prevent apoptosis to enhance survival of mucosal cells to subsequently provide enhanced mucosal barrier function [5].…”

Section: Introductionmentioning

confidence: 99%

Lactobacillus plantarum 299v Prevents Caspase-Dependent Apoptosis In Vitro

Dykstra

Hyde

Mackenzie

et al. 2011

Probiotics & Antimicro. Prot.

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Selective microbes used as probiotics can enhance epithelial cell protection. We have previously shown that a Lactobacillus plantarum strain 299v (Lp299v) has the ability to induce mucin genes. In the current study, we utilized a cytokine model of inflammation in cell culture to study the modulation of apoptosis by this probiotic. HT-29 cells were pre-incubated with the Lp299v or L. plantarum strain adh- (Lpadh-), a non-adherent derivative of Lp299v. Cells were challenged with a mixture of cytokines (TNF-α, IFN-γ, and IL-1a) to imitate conditions of inflammation. To assess for cell death, we evaluated TUNEL, multi-caspase, and caspase-3 and caspase-7 activity assays. There was a marked decrease in apoptosis as measured by TUNEL(+) cells in samples pre-treated with Lp299v (18.7 ± 4.1%, p < 0.01) and Lpadh- (16.6 ± 3.2%, p < 0.05) prior to cytokine exposure when compared to cells (43.6 ± 6.2%) exposed to the cytokine mixture. Lp299v pre-incubation with HT-29 cells reduced caspase(+) cells in the multi-caspase activity assay (3.6 ± 0.6%, p < 0.05) compared to cells exposed to cytokines (68.9 ± 5.1%) whereas Lpadh- did not (46.8 ± 17.5%, p > 0.05). Similarly, caspase-3, caspase-7 activity was also reduced by Lp299v. Selected probiotics may confer an exogenous protective effect at the mucosal-luminal interface for intestinal epithelial cells via alteration of caspase-dependent apoptotic pathways.

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Lactobacillus plantarum Inhibits the Intestinal Epithelial Migration of Neutrophils Induced by Enteropathogenic Escherichia coli

Cited by 40 publications

References 24 publications

Pulse Probiotic Administration Induces Repeated Small Intestinal Muc3 Expression in Rats

Pulse Probiotic Administration Induces Repeated Small Intestinal Muc3 Expression in Rats

Lactobacillus plantarum—survival, functional and potential probiotic properties in the human intestinal tract

Lactobacillus plantarum 299v Prevents Caspase-Dependent Apoptosis In Vitro

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