Lactobacillus plantarum ST-III culture supernatant ameliorates alcohol-induced cognitive dysfunction by reducing endoplasmic reticulum stress and oxidative stress

Abstract

BackgroundLong-term alcohol exposure is associated with oxidative stress, endoplasmic reticulum (ER) stress, and neuroinflammation, which may impair cognitive function. Probiotics supplements can significantly improve cognitive function in neurodegenerative diseases such as Alzheimer’s disease. Nevertheless, the effect of Lactobacillus plantarum ST-III culture supernatant (LP-cs) on alcohol-induced cognitive dysfunction remains unclear.MethodsA mouse model of cognitive dysfunction was established by intraperitoneal injection of alcohol (2 g/kg body weight) for 28 days. Mice were pre-treated with LP-cs, and cognitive function was evaluated using the Morris water maze test. Hippocampal tissues were collected for biochemical and molecular analysis.ResultsLP-cs significantly ameliorated alcohol-induced decline in learning and memory function and hippocampal morphology changes, neuronal apoptosis, and synaptic dysfunction. A mechanistic study showed that alcohol activated protein kinase R-like endoplasmic reticulum kinase (PERK) signaling and suppressed brain derived neurotrophic factor (BDNF) levels via ER stress in the hippocampus, which LP-cs reversed. Alcohol activated oxidative stress and inflammation responses in the hippocampus, which LP-cs reversed.ConclusionLP-cs significantly ameliorated alcohol-induced cognitive dysfunction and cellular stress. LP-cs might serve as an effective treatment for alcohol-induced cognitive dysfunction.

Mechanism Explanation on Improved Cognitive Ability of D-Gal Inducing Aged Mice Model by Lactiplantibacillus plantarum MWFLp-182 via the Microbiota–Gut–Brain Axis

“…25 Oral administration of Lactobacillus plantarum ST-III was shown to improve cognitive function by upregulating the expression of BDNF and relieving inflammatory responses in the hippocampus. 45 In our study, treatment with L. plantarum MWFLp-182 activated the protein expression of PSD-95, NRF2, and NGF, increased the BDNF + /NeuN + cell population and BDNF expression, and decreased Bax expression in the hippocampus, thereby ameliorating the D-gal-induced cognitive injury in mice (Figure 7). The gut microbiota, the key node of the gut−brain axis, is associated with neurotransmission, complex cognitive behaviors, and BDNF function.…”

“…In addition, Lactobacillus mucosae NK41 and Bifidobacterium longum NK46 could alleviate cognitive impairment by increasing the BDNF + /NeuN + cell population . Oral administration of Lactobacillus plantarum ST-III was shown to improve cognitive function by upregulating the expression of BDNF and relieving inflammatory responses in the hippocampus . In our study, treatment with L.…”

Lactiplantibacillus (Lactobacillus) plantarum as a Complementary Treatment to Improve Symptomatology in Neurodegenerative Disease: A Systematic Review of Open Access Literature

“…A total of 17 studies were conducted using different animal models. Of these, 4 focused on AD [ 95 , 96 , 97 , 98 ] ( n = 4); 7 focused on PD [ 99 , 100 , 101 , 102 , 103 , 104 , 105 ] ( n = 7); 1 analyzed MS [ 106 ], ( n = 1); 3 studied oxidative stress [ 107 , 108 , 109 ] ( n = 3); 1 on cognitive impairment and oxidative stress [ 110 ] ( n = 1); and 1 on neurodegenerative process [ 111 ] ( n = 1). All of them addressed the intervention with Lactobacillus plantarum or Lactiplantibacillus plantarum for the improvement of symptoms associated with neurodegenerative pathologies.…”

Protection of LP-cs on acute alcohol-induced liver and intestine injury

“…According to the instructions, LP was activated and passaged three times in MRS medium, inoculated in 100 mL liquid medium according to 3% inoculation amount of culture medium, cultured at 37 ℃, 5% CO 2 for 24 hours, centrifuged 10min at 4 ℃ and 5000 r/min, and the supernatant was ltered through 0.22-µm lter to obtain extracellular uid. This procedure was performed as described previously [17,24].…”

“…Male ICR mice (about 20g) were obtained from Vital River Laboratory Animal Technology Co., Ltd, Shanghai. All mice were maintained and fed as described previously [17,24]. The LP-cs was added into the drinking water at a ratio (1:20), which was consumed about 8 ~ 9 ml/mouse per day.…”