Lactobacillus plantarum USM8613 Aids in Wound Healing and Suppresses Staphylococcus aureus Infection at Wound Sites

Ong, Jia Sin; Taylor, Todd D.; Yong, Cheng Chung; Khoo, Boon Yin; Sasidharan, Sreenivasan; Choi, Sy Bing; Ohno, Hiroshi; Liong, Min‐Tze

doi:10.1007/s12602-018-9505-9

Cited by 71 publications

(61 citation statements)

References 32 publications

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“…Probiotics have demonstrated the ability in multiple human and animal models to improve wound-healing efficacy. [33][34][35][36][37][38][39][40][41][42] Probiotics of investigation have included L. plantarum, kefir, L. fermentum, and S. cerevisiae in thermal injury models, infected and non-infected wounds, and diabetic ulcers. In these studies, the mechanism of action was typically not explored, but topical probiotic treatment resulted in improved healing as demonstrated by increased granulation tissue deposition, improved collagen concentration, and stimulation of angiogenesis.…”

Section: Wound Healing and Topical Probiotic: General Studiesmentioning

confidence: 99%

“…In these studies, the mechanism of action was typically not explored, but topical probiotic treatment resulted in improved healing as demonstrated by increased granulation tissue deposition, improved collagen concentration, and stimulation of angiogenesis. [33][34][35][36][37][38][39][40][41][42] However, not all models demonstrated an improvement in topical wound healing with probiotics [33][34][35][36][37][38][39][40][41][42][43][44] (Tables 1 and 2).…”

Section: Wound Healing and Topical Probiotic: General Studiesmentioning

confidence: 99%

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The role of topical probiotics on wound healing: A review of animal and human studies

Knackstedt

Gatherwright

2020

International Wound Journal

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Pathogenic, opportunistic, and commensal bacterial coexist in the intestinal tract, and imbalances among these strains have been linked to systemic inflammation and a variety of disease states. Similarly, human skin plays an important role as an interface between the body and the environment with an estimated 1 billion microbes per square centimetres. Skin microbiome fluctuations that cause increases in pathologic bacteria, either because of individual and/or environmental factors, can lead to disease states at the skin level ranging from inflammatory conditions to infections. As wounds are inherently associated with perturbations in the local microflora due to injury and activation of the immune responses, the addition of topical probiotics could be a means to prevent infection, regulate inflammation, and potentially augment healing. The goal of this review is to analyse the impact the skin microbiome has on cutaneous wound healing with a focus on developing proposed treatment algorithms and support for their therapeutic potential.

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Section: Wound Healing and Topical Probiotic: General Studiesmentioning

confidence: 99%

Section: Wound Healing and Topical Probiotic: General Studiesmentioning

confidence: 99%

The role of topical probiotics on wound healing: A review of animal and human studies

Knackstedt

Gatherwright

2020

International Wound Journal

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“…Staphylococcus epidermidis protects the skin from pathogen infections by secreting antimicrobial peptides (76), although, in some specific conditions, it may become an opportunistic pathogen. Lactic acid bacteria have also been associated with skin wound healing because they can produce bioactive metabolites that control pathogen bacteria and modulate the immune response (77). The ability of commensal S. epidermidis to contribute to wound healing was recently examined by Li et al (24).…”

Section: Staphylococcus Epidermidis: Inhibition Of Skin Inflammation mentioning

confidence: 99%

Wnt/β-Catenin Signaling as a Molecular Target by Pathogenic Bacteria

2019

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The Wnt/β-catenin signaling pathway is crucial to regulate cell proliferation and polarity, cell determination, and tissue homeostasis. The activation of Wnt/β-catenin signaling is based on the interaction between Wnt glycoproteins and seven transmembrane receptors—Frizzled (Fzd). This binding promotes recruitment of the scaffolding protein Disheveled (Dvl), which results in the phosphorylation of the co-receptor LRP5/6. The resultant molecular complex Wnt–Fzd–LRP5/6-Dvl forms a structural region for Axin interaction that disrupts Axin-mediated phosphorylation/degradation of the transcriptional co-activator β-catenin, thereby allowing it to stabilize and accumulate in the nucleus where it activates the expression of Wnt-dependent genes. Due to the prominent physiological function, the Wnt/β-catenin signaling must be strictly controlled because its dysregulation, which is caused by different stimuli, may lead to alterations in cell proliferation, apoptosis, and inflammation-associated cancer. The virulence factors from pathogenic bacteria such as Salmonella enterica sv Typhimurium, Helicobacter pylori, Mycobacterium tuberculosis, Pseudomonas aeruginosa, Citrobacter rodentium, Clostridium difficile, Bacteroides fragilis, Escherichia coli, Haemophilus parasuis, Lawsonia intracellularis, Shigella dysenteriae, and Staphylococcus epidermidis employ a variety of molecular strategies to alter the appropriate functioning of diverse signaling pathways. Among these, Wnt/β-catenin has recently emerged as an important target of several virulence factors produced by bacteria. The mechanisms used by these factors to interfere with the activity of Wnt/β-catenin is diverse and include the repression of Wnt inhibitors' expression by the epigenetic modification of histones, blocking Wnt–Fzd ligand binding, activation or inhibition of β-catenin nuclear translocation, down- or up-regulation of Wnt family members, and inhibition of Axin-1 expression that promotes β-catenin activity. Such a variety of mechanisms illustrate an evolutionary co-adaptation of eukaryotic molecular signaling to a battery of soluble or structural components synthesized by pathogenic bacteria. This review gathers the recent efforts to elucidate the mechanistic details through which bacterial virulence factors modulate Wnt/β-catenin signaling and its physiological consequences concerning the inflammatory response and cancer.

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“…e studies investigated burn wounds, ischemic wounds, and skin lesions. ree studies each used mouse [61,68,78] and rat models [76,80,81] and two studies used rabbit models [77,79]. Local application of probiotics was used for six studies and only two studies included local injections [61,78] of probiotics.…”

Section: Animal Studies On Use Of Probiotics For Wound Infectionsmentioning

confidence: 99%

Efficacy of Using Probiotics with Antagonistic Activity against Pathogens of Wound Infections: An Integrative Review of Literature

Fijan

Frauwallner²,

Langerholc

et al. 2019

BioMed Research International

104

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e skin and its microbiota serve as physical barriers to prevent invasion of pathogens. Skin damage can be a consequence of illness, surgery, and burns. e most effective wound management strategy is to prevent infections, promote healing, and prevent excess scarring. It is well established that probiotics can aid in skin healing by stimulating the production of immune cells, and they also exhibit antagonistic effects against pathogens via competitive exclusion of pathogens. Our aim was to conduct a review of recent literature on the efficacy of using probiotics against pathogens that cause wound infections. In this integrative review, we searched through the literature published in the international following databases: PubMed, ScienceDirect, Web of Science, and Scopus using the search terms "probiotic" AND "wound infection." During a comprehensive review and critique of the selected research, fourteen in vitro studies, 8 animal studies, and 19 clinical studies were found. Two of these in vitro studies also included animal studies, yielding a total of 39 articles for inclusion in the review. e most commonly used probiotics for all studies were well-known strains of the species Lactobacillus plantarum, Lactobacillus casei, Lactobacillus acidophilus, and Lactobacillus rhamnosus. All in vitro studies showed successful inhibition of chosen skin or wound pathogens by the selected probiotics. Within the animal studies on mice, rats, and rabbits, probiotics showed strong opportunities for counteracting wound infections. Most clinical studies showed slight or statistically significant lower incidence of surgical site infections, foot ulcer infection, or burn infections for patients using probiotics. Several of these studies also indicated a statistically significant wound healing effect for the probiotic groups. is review indicates that exogenous and oral application of probiotics has shown reduction in wound infections, especially when used as an adjuvant to antibiotic therapy, and therefore the potential use of probiotics in this field remains worthy of further studies, perhaps focused more on typical skin inhabitants as next-generation probiotics with high potential.

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Lactobacillus plantarum USM8613 Aids in Wound Healing and Suppresses Staphylococcus aureus Infection at Wound Sites

Cited by 71 publications

References 32 publications

The role of topical probiotics on wound healing: A review of animal and human studies

The role of topical probiotics on wound healing: A review of animal and human studies

Wnt/β-Catenin Signaling as a Molecular Target by Pathogenic Bacteria

Efficacy of Using Probiotics with Antagonistic Activity against Pathogens of Wound Infections: An Integrative Review of Literature

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