Lactobacillus reuteri DSM 17938 alleviates d-galactosamine-induced liver failure in rats

Jiang, Huiyong; Ren, Yan; Wang, Kaicen; Wang, Qiangqiang; Chen, Xiaoxiao; Chen, Lifeng; Li, Lanjuan; Lv, Longxian

doi:10.1016/j.biopha.2020.111000

Cited by 36 publications

(35 citation statements)

References 30 publications

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“…The 16S rDNA V3-V4 region was amplified by PCR using the primers 338F (5’-ACTCCTACGGGAGGCAGCAG-3’) and 806R (5’-GGACTACHVGGGTWTCTAAT-3’). PCR was performed under the following conditions: start at 95°C for 5 min; 25 cycles at 94°C for 50 s, 46°C for 1 min, and 72°C for 30 s; and a final extension step at 72°C for 5 min ( 14 , 15 ). The libraries were prepared for Illumina sequencing using a NEXTflex rapid DNA-seq kit (Bioo Scientific, Austin, TX, USA) according the manufacturer’s instructions.…”

Section: Methodsmentioning

confidence: 99%

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Mechanism of the Immunomodulatory Effect of the Combination of Live Bifidobacterium, Lactobacillus, Enterococcus, and Bacillus on Immunocompromised Rats

et al. 2021

Front. Immunol.

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Immunodeficiency is a very common condition in suboptimal health status and during the development or treatment of many diseases. Recently, probiotics have become an important means for immune regulation. The present study aimed to investigate the mechanism of the immunomodulatory effect of a combination of live Bifidobacterium, Lactobacillus, Enterococcus, and Bacillus (CBLEB), which is a drug used by approximately 10 million patients every year, on cyclophosphamide-immunosuppressed rats. Cyclophosphamide (40 mg/kg) was intraperitoneally injected to induce immunosuppression in a rat model on days 1, 2, 3, and 10. Starting from day 4, the rats were continuously gavaged with CBLEB solution for 15 days. The samples were collected to determine routine blood test parameters, liver and kidney functions, serum cytokine levels, gut microbiota, fecal and serum metabolomes, transcriptomes, and histopathological features. The results indicated that CBLEB treatment reduced cyclophosphamide-induced death, weight loss, and damage to the gut, liver, spleen, and lungs and eliminated a cyclophosphamide-induced increase in the mean hemoglobin content and GGT, M-CSF, and MIP-3α levels and a decrease in the red blood cell distribution width and total protein and creatinine levels in the blood. Additionally, CBLEB corrected cyclophosphamide-induced dysbiosis of the gut microbiota and eliminated all cyclophosphamide-induced alterations at the phylum level in rat feces, including the enrichment in Proteobacteria, Fusobacteriota, and Actinobacteriota and depletion of Spirochaetota and Cyanobacteria. Furthermore, CBLEB treatment alleviated cyclophosphamide-induced alterations in the whole fecal metabolome profile, including enrichment in 1-heptadecanol, succinic acid, hexadecane-1,2-diol, nonadecanoic acid, and pentadecanoic acid and depletion of benzenepropanoic acid and hexane. CBLEB treatment also alleviated cyclophosphamide-induced enrichment in serum D-lyxose and depletion of serum succinic acid, D-galactose, L-5-oxoproline, L-alanine, and malic acid. The results of transcriptome analysis indicated that the mechanism of the effect of CBLEB was related to the induction of recovery of cyclophosphamide-altered carbohydrate metabolism and signal transduction. In conclusion, the present study provides an experimental basis and comprehensive analysis of application of CBLEB for the treatment of immunodeficiency.

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Section: Methodsmentioning

confidence: 99%

“…The sections were scanned with a section scanner, and the images were used to evaluate the degree of tissue damage, inflammation, and necrosis in the liver, spleen, lung, and colon. Hepatic injuries were assessed based on the histological activity index (HAI) ( 15 ). Intestinal mucosal injury was graded from 0 to 5 points ( 14 ).…”

Section: Methodsmentioning

confidence: 99%

Mechanism of the Immunomodulatory Effect of the Combination of Live Bifidobacterium, Lactobacillus, Enterococcus, and Bacillus on Immunocompromised Rats

et al. 2021

Front. Immunol.

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“…Beside our work, accumulating evidence suggests that D-GalN treatment not just induces hepatocyte damage but also destroys the gut microbiota homeostasis and the intestinal barrier structure by allowing MAMPs, such as LPS, to enter the liver and peripheral blood (Li et al, 2004 ). Conversely, pretreatment with probiotic Lactobacillus reuteri DSM 17938 can relieve the gut dysbiosis, reduce the transcription of inflammatory factors, and alleviate D-GalN-induced liver injury in rats (Jiang et al, 2021 ). Probiotic Lactobacillus casei Zhang can also reduce LPS/D-GalN-induced pro-inflammatory cytokines and hepatic inflammation through the modulation of the TLR-MAPK-PPAR-γ signaling pathways (Wang et al, 2016 ).…”

Section: The Gut Microbiota and D-galactosamine-induced Acute Liver Injurymentioning

confidence: 99%

“…However, compared with ALI, these studies mostly focused on chronic liver diseases, such as non-alcoholic fatty liver disease (NAFLD), autoimmune hepatitis, liver cirrhosis, and hepatocellular carcinoma (Compare et al, 2012 ; Lee and Suk, 2020 ; Wei et al, 2020 ). In recent years, an increasing number of preventive or adjunct therapeutic measures for ALI have been proposed based on the regulation of gut microbiota (Kim et al, 2018 ; Jiang et al, 2021 ). The gut contains around 1,000 different species of bacteria, which play a crucial role in the survival of organisms.…”

Section: Introductionmentioning

confidence: 99%

Gut Microbiota and Chemical-Induced Acute Liver Injury

Chen

2021

Front. Physiol.

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Background: Drug overdose or chemical exposures are the main causes of acute liver injury (ALI). Severe liver injury can develop into liver failure that is an important cause of liver-related mortality in intensive care units in most countries. Pharmacological studies have utilized a variety of comprehensive chemical induction models that recapitulate the natural pathogenesis of acute liver injury. Their mechanism is always based on redox imbalance-induced direct hepatotoxicity and massive hepatocyte cell death, which can trigger immune cell activation and recruitment to the liver. However, the pathogenesis of these models has not been fully stated. Many studies showed that gut microbiota plays a crucial role in chemical-induced liver injury. Hepatotoxicity is likely induced by imbalanced microbiota homeostasis, gut mucosal barrier damage, systemic immune activation, microbial-associated molecular patterns, and bacterial metabolites. Meanwhile, many preclinical studies have shown that supplementation with probiotics can improve chemical-induced liver injury. In this review, we highlight the pathogenesis of gut microorganisms in chemical-induced acute liver injury animal models and explore the protective mechanism of exogenous microbial supplements on acute liver injury.

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“…The libraries were sequenced using a 300PE MiSeq sequencing kit with the standard Illumina sequencing primers and the Illumina MiSeq platform (Illumina, CA, USA). Data processing was conducted as described previously ( 3 , 17 , 18 ). Briefly, paired-end reads were merged using FLASH v.1.2.11.…”

Section: Methodsmentioning

confidence: 99%

The Salivary Microbiota of Patients With Primary Biliary Cholangitis Is Distinctive and Pathogenic

Jiang

Chen

et al. 2021

Front. Immunol.

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The role of host-microbiota interactions in primary biliary cholangitis (PBC) has received increased attention. However, the impact of PBC on the oral microbiota and contribution of the oral microbiota to PBC are unclear. In this study, thirty-nine PBC patients without other diseases and 37 healthy controls (HCs) were enrolled and tested for liver functions and haematological variables. Saliva specimens were collected before and after brushing, microbiota was determined using 16S rDNA sequencing, metabolomics was profiled using Gas Chromatography-Mass Spectrometer (GC-MS), 80 cytokines were assayed using biochips, and inflammation inducibility was evaluated using OKF6 keratinocytes and THP-1 macrophages. Finally, the effect of ultrasonic scaling on PBC was estimated. Compared with HCs, PBC saliva had enriched taxa such as Bacteroidetes, Campylobacter, Prevotella and Veillonella and depleted taxa such as Enterococcaceae, Granulicatella, Rothia and Streptococcus. PBC saliva also had enriched sCD163, enriched metabolites such as 2-aminomalonic acid and 1-dodecanol, and depleted metabolites such as dodecanoic acid and propylene glycol. sCD163, 4-hydroxybenzeneacetic acid and 2-aminomalonic acid were significantly correlated with salivary cytokines, bacteria and metabolites. Salivary Veillonellaceae members, 2-aminomalonic acid, and sCD163 were positively correlated with liver function indicators such as serum alkaline phosphatase (ALP), aspartate aminotransferase (AST) and alanine aminotransferase (ALT). PBC salivary microbes induced more soluble interleukin (IL)-6 receptor α (sIL-6Rα), sIL-6Rβ and tumour necrosis factor ligand superfamily (TNFSF)13B from OKF6 keratinocytes, and PBC salivary supernatant induced more IL-6, IL-10, granulocyte-macrophage colony-stimulating factor (GM-CSF), chemokine (C-C motif) ligand (CCL)13, C-X-C motif chemokine (CXC)L1 and CXCL16 from THP-1 macrophages. Toothbrushing significantly reduced the expression of inflammatory cytokines such as IL-1β, IL-8 and TNF-α and harmful metabolites such as cadaverine and putrescine in PBC but not HC saliva after P‐value correction. The levels of ALP and bilirubin in PBC serum were decreased after ultrasonic scaling. Together, PBC patients show significant alterations in their salivary microbiota, likely representing one cause and treatment target of oral inflammation and worsening liver functions.

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Lactobacillus reuteri DSM 17938 alleviates d-galactosamine-induced liver failure in rats

Cited by 36 publications

References 30 publications

Mechanism of the Immunomodulatory Effect of the Combination of Live Bifidobacterium, Lactobacillus, Enterococcus, and Bacillus on Immunocompromised Rats

Mechanism of the Immunomodulatory Effect of the Combination of Live Bifidobacterium, Lactobacillus, Enterococcus, and Bacillus on Immunocompromised Rats

Gut Microbiota and Chemical-Induced Acute Liver Injury

The Salivary Microbiota of Patients With Primary Biliary Cholangitis Is Distinctive and Pathogenic

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