Lactobacillus rhamnosus and Bifidobacterium longum alleviate colitis and cognitive impairment in mice by regulating IFN-γ to IL-10 and TNF-α to IL-10 expression ratios

Ma, Xiaoyang; Jang, Hyo Min; Joo, Min Kyung; Yoo, Jong Wook; Kim, Dong Hyun

doi:10.1038/s41598-021-00096-x

Cited by 32 publications

(15 citation statements)

References 46 publications

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“…Probiotics, including Lactobacilli and Bifidobacteria, alleviate gut dysbiosis [ 17 ], immune imbalance [ 18 ], inflammation [ 19 ], depression [ 20 ], and memory impairment [ 21 ]. Bifidobacterium adolescentis IM38 alleviated IS-induced anxiety and high-fat-diet-induced gut inflammation in mice [ 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

The Alleviation of Gut Microbiota-Induced Depression and Colitis in Mice by Anti-Inflammatory Probiotics NK151, NK173, and NK175

Yoo

Shin

et al. 2022

Nutrients

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Gut microbiota dysbiosis is strongly associated with psychiatric disorders and inflammatory bowel disease (IBD). Herein, we examined whether the fecal microbiota of IBD patients with depression (IBDD) and their gut microbiota culture (iGm) could cause depression and colitis in mice and anti-inflammatory probiotics could mitigate depression in iGm-transplanted or immobilization stress (IS)-exposed mice. Fecal microbiota transplantation (FMT) from IBDD patients, which exhibited Enterobacteriaceae-rich gut microbiota, and its gut microbiota culture (iGm) increased depression-like behaviors in mice. Their treatments heightened the blood lipopolysaccharide (LPS) level and colonic IL-1β and IL-6 expression. However, FMT from healthy volunteers or sulfasalazine treatment alleviated cGm-induced depressive-like behaviors and hippocampal and colonic inflammation in mice. Moreover, oral administration of Lactobacillus plantarum NK151, Bifidobacterium longum NK173, and Bifidobacterium bifidum NK175, which inhibited LPS-induced IL-6 expression in macrophages, alleviated cGm-induced depression-like behaviors, hippocampal NF-κB+Iba1+ cell numbers and IL-1β and IL-6 expression, blood LPS, IL-6, and creatinine levels, and colonic NF-κB+CD11c+ number and IL-1β and IL-6 expression in mice. Treatment with NK151, NK173, or NK175 mitigated immobilization stress (IS)-induced depressive-like behaviors, neuroinflammation, and gut inflammation in mice. NK151, NK173, or NK175 also decreased IS-induced blood LPS, IL-6, and creatinine levels. The transplantation of Enterobacteriaceae-rich gut microbiota can cause depression and colitis, as IS exposure, and anti-inflammatory NK151, NK173, and NK175, may alleviate stress-induced fatigue, depression, and colitis by regulating the expression of proinflammatory and anti-inflammatory cytokines through the suppression of gut bacterial LPS.

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Section: Introductionmentioning

confidence: 99%

The Alleviation of Gut Microbiota-Induced Depression and Colitis in Mice by Anti-Inflammatory Probiotics NK151, NK173, and NK175

Yoo

Shin

et al. 2022

Nutrients

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“…In contrast, Blastocystis ST7 disrupted tight junction proteins such as occludin and ZO-1 [ 48 , 49 ] as well as increases the levels of pro-inflammatory cytokines to trigger an inflammatory response [ 50 , 51 ]. In addition, Lactobacillus rhamnosus and Bifidobacterium longum have shown to alleviate cognitive impairment in mice by regulation the IFN-γ/IL-10 and TNF-α/IL-10 rations [ 52 ], whereas Lactobacillus plantarum and Bifidobacterium longum have also shown to alleviate cognitive impairment in mice by up-regulating NF-kB-mediated BDNF expression [ 43 ]. A recent study has shown that Bifidobacterium breve prevented memory impairment a mouse model with phenotypic features of Alzheimer’s disease through microglia activation and the reduction of amyloid-β production [ 53 ].…”

Section: Discussionmentioning

confidence: 99%

Presence of Blastocystis in gut microbiota is associated with cognitive traits and decreased executive function

et al. 2022

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Growing evidence implicates the gut microbiome in cognition. Blastocystis is a common gut single-cell eukaryote parasite frequently detected in humans but its potential involvement in human pathophysiology has been poorly characterized. Here we describe how the presence of Blastocystis in the gut microbiome was associated with deficits in executive function and altered gut bacterial composition in a discovery (n = 114) and replication cohorts (n = 942). We also found that Blastocystis was linked to bacterial functions related to aromatic amino acids metabolism and folate-mediated pyrimidine and one-carbon metabolism. Blastocystis-associated shifts in bacterial functionality translated into the circulating metabolome. Finally, we evaluated the effects of microbiota transplantation. Donor’s Blastocystis subtypes led to altered recipient’s mice cognitive function and prefrontal cortex gene expression. In summary, Blastocystis warrant further consideration as a novel actor in the gut microbiome-brain axis.

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“…NK209, NK210, and NK219 were cultured in commercial media for probiotics including MRS broth (BD, Franklin Lakes, NJ, USA), centrifuged at 5000× g and at 4 °C for 20 min, and washed with saline [ 23 , 24 ]. The collected cells were used for experiments.…”

Section: Methodsmentioning

confidence: 99%

“…The YMT was performed in a three-arm horizontal maze (40 cm (length), 3 cm (width) and 12 cm (height)) for 8 min, as previously reported [ 19 , 23 ]. The spontaneous alteration (%) was described as a spontaneous to possible alternation ratio.…”

Section: Methodsmentioning

confidence: 99%

Tight Junction Protein Expression-Inducing Probiotics Alleviate TNBS-Induced Cognitive Impairment with Colitis in Mice

Son²,

Yoo³

et al. 2022

Nutrients

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A leaky gut is closely connected with systemic inflammation and psychiatric disorder. The rectal injection of 2,4,6-trinitrobenzenesulfonic acid (TNBS) induces gut inflammation and cognitive function in mice. Therefore, we selected Bifidobacterium longum NK219, Lactococcus lactis NK209, and Lactobacillus rhamnosus NK210, which induced claudin-1 expression in TNBS- or lipopolysaccharide (LPS)-stimulated Caco-2 cells, from the fecal bacteria collection of humans and investigated their effects on cognitive function and systemic inflammatory immune response in TNBS-treated mice. The intrarectal injection of TNBS increased cognitive impairment-like behaviors in the novel object recognition and Y-maze tests, TNF-α, IL-1β, and IL-17 expression in the hippocampus and colon, and LPS level in the blood and feces, while the expression of hippocampal claudin-5 and colonic claudin-1 decreased. Oral administration of NK209, NK210, and NK219 singly or together decreased TNBS-impaired cognitive behaviors, TNF-α and IL-1β expression, NF-κB+Iba1+ cell and LPS+Iba1+ cell numbers in the hippocampus, and LPS level in the blood and feces, whereas BDNF+NeuN+ cell and claudin-5+ cell numbers and IL-10 expression increased. Furthermore, they suppressed TNBS-induced colon shortening and colonic TNF-α and IL-1β expression, while colonic IL-10 expression and mucin protein-2+ cell and claudin-1+ cell numbers expression increased. Of these, NK219 most strongly alleviated cognitive impairment and colitis. They additively alleviated cognitive impairment with colitis. Based on these findings, NK209, NK210, NK219, and their combinations may alleviate cognitive impairment with systemic inflammation by suppressing the absorption of gut bacterial products including LPS into the blood through the suppression of gut bacterial LPS production and alleviation of a leaky gut by increasing gut tight junction proteins and mucin-2 expression.

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Lactobacillus rhamnosus and Bifidobacterium longum alleviate colitis and cognitive impairment in mice by regulating IFN-γ to IL-10 and TNF-α to IL-10 expression ratios

Cited by 32 publications

References 46 publications

The Alleviation of Gut Microbiota-Induced Depression and Colitis in Mice by Anti-Inflammatory Probiotics NK151, NK173, and NK175

The Alleviation of Gut Microbiota-Induced Depression and Colitis in Mice by Anti-Inflammatory Probiotics NK151, NK173, and NK175

Presence of Blastocystis in gut microbiota is associated with cognitive traits and decreased executive function

Tight Junction Protein Expression-Inducing Probiotics Alleviate TNBS-Induced Cognitive Impairment with Colitis in Mice

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