Lactobacillus rhamnosus could inhibit Porphyromonas gingivalis derived CXCL8 attenuation

Abstract

An increasing body of evidence suggests that the use of probiotic bacteria is a promising intervention approach for the treatment of inflammatory diseases with a polymicrobial etiology. P. gingivalis has been noted to have a different way of interacting with the innate immune response of the host compared to other pathogenic bacteria, which is a recognized feature that inhibits CXCL8 expression. Objective The aim of the study was to determine if P. gingivalis infection modulates the inflammatory response of gingival stromal stem cells (G-MSSCs), including the release of CXCL8, and the expression of TLRs and if immunomodulatory L. rhamnosus ATCC9595 could prevent CXCL8 inhibition in experimental inflammation.Material and Methods G-MSSCs were pretreated with L. rhamnosus ATCC9595 and then stimulated with P. gingivalis ATCC33277. CXCL8 and IL-10 levels were investigated with ELISA and the TLR-4 and 2 were determined through flow cytometer analysis.Results CXCL8 was suppressed by P. gingivalis and L. rhamnosus ATCC9595, whereas incubation with both strains did not abolish CXCL8. L. rhamnosus ATCC9595 scaled down the expression of TLR4 and induced TLR2 expression when exposed to P. gingivalis stimulation (p<0.01).Conclusions These findings provide evidence that L. rhamnosus ATCC9595 can modulate the inflammatory signals and could introduce P. gingivalis to immune systems by inducing CXCL8 secretion.

Impact of maternal intrapartum antibiotics on the initial oral microbiome of neonates

“…31 This is likely due to its immune modulation; recent in vitro studies showed that probiotic Lactobacillus could abolish CXCL8 attenuation to promote Th1 and Th17 responses. 32,33 However, that abundance decreased significantly in the AT group demonstrated that maternal intrapartum antibiotics exposure was associated with decreased transmission of Lactobacillus to the neonate. There was also a slightly lower microbial diversity in antibiotics-free infants, which might be attributed to the relative abundance discrepancy of microbiome between the AT and NT groups, for there were much higher presence of Lactobacillus among neonates without maternal intrapartum antibiotics administration.…”

In vitro beneficial effects of Streptococcus dentisani as potential oral probiotic for periodontal diseases

“…The most common probiotics strains used in oral health belong to the genera Bifidobacterium and Lactobacillus . ‒ For instance, when incorporated to commercial toothpastes, L. casei and L. acidophilus were able to in vitro inhibit Candida albicans and Streptococcus salivarius growth .…”

“…1,31 The most common probiotics strains used in oral health belong to the genera Bifidobacterium and Lactobacillus. 20,30,[32][33][34] For instance, when incorporated to commercial toothpastes, L. casei and L. acidophilus were able to in vitro inhibit Candida albicans and Streptococcus salivarius growth. 33 Recently, it has been hypothesized that oral commensals associated with health are likely to be more effective as probiotic species than the traditional gut-associated probiotic species in terms of ability to colonization, health-promoting functions, biocompatibility, and prophylactic dosage.…”

Use of Probiotics and Oral Health

“…Other studies have also shown protective effects against bone loss with Bacillus subtilis , Bacillus licheniformis and L. brevis CD2 in murine periodontitis models [ 46 – 48 ]. Recent in vitro studies continue to elucidate the mechanisms of probiotic immune modulation, showing that probiotic lactobacilli could abolish CXCL8 attenuation by P. gingivalis , and promote Th1 and Th17 responses [ 49 , 50 ]. An 8-species probiotic mixture containing lactobacilli, bifidobacteria and streptococcus has been shown to polarise human macrophages towards the M1-phenotype [ 51 ].…”