Lactobacillus rhamnosus Probio-M9 Improves the Quality of Life in Stressed Adults by Gut Microbiota

Abstract

To evaluate the effect of the probiotic, Lactobacillus rhamnosus Probio-M9 (Probio-M9), on the quality of life in stressed adults. Methods: Twelve postgraduate student volunteers were recruited. Six volunteers received oral Probio-M9 for 21 days, while the remaining six received a placebo instead. Fecal samples were collected from the volunteers before and after the intervention. Metagenomic sequencing, nontargeted metabonomics, and quality-of-life follow-up questionnaires were used to evaluate the impact of Probio-M9 consumption on the gut microbiota and life quality of the volunteers. Results: Probio-M9 improved the psychological and physiological quality-of-life symptoms significantly in stressed adults (p < 0.05). The probiotic intervention was beneficial in increasing and maintaining the diversity of gut microbiota. The abundance of Barnesiella and Akkermansia increased in the probiotics group. The feature metabolites of pyridoxamine, dopamine, and 5-hydroxytryptamine (5-HT) were positively correlated with Barnesiella and Akkermansia levels, which might be why the mental state of the volunteers in the probiotic group improved after taking Probio-M9. Conclusions: We identified that oral Probio-M9 can regulate the stability of gut microbiota and affect the related beneficial metabolites, thereby affecting the quality of life (QoL) of stressed adults. Probio-M9 might improve the psychological and physiological quality of life in stressed adults via the gut-brain axis pathway. The causal relationship should be further explored in future studies.

Change in the Gut Microbiome and Immunity by Lacticaseibacillusrhamnosus Probio-M9

“…To our knowledge, the present research is the first to report the underestimated alteration of association between immunity and the gut microbiome/metabolome, as well as the suppressed SNVs by probiotic consumption in nonpatients. Despite many studies that have been conducted on Lacticaseibacillus rhamnosus Probio-M9 (such as its isolation process, biochemical characteristics [ 60 ], and mouse [ 61 – 63 ], and small-scale human experiments [ 64 ] examining its beneficial effects on hosts), the comprehensive evaluation of Probio-M9 using multiomics data collected from different time points, as presented in this article, greatly compensates for the gaps in probiotic research and significantly increases our understanding of the mechanisms behind the probiotic beneficial effects. However, there are some limitations to this study.…”

“… Lacticaseibacillus rhamnosus Probio-M9 (5.0 × 10 10 CFU per day) was used for the probiotic intervention; Probio-M9 is a probiotic isolated from healthy women’s breast milk ( 60 , 66 , 67 ). We selected this strain because it has no transferable antibiotic resistance genes ( 60 ) and is potentially beneficial for easing the symptoms of anxiety, stress, and gastrointestinal distress ( 64 , 68 ). However, little effort has been made to comprehensively understand its mechanism: e.g., its changes in the genomic structure after administration.…”

“…Sample preparation for ultraperformance liquid chromatography-quadrupole time of flight mass spectrometry (UPLC-QTOF/MSE) analysis was performed as previously described ( 64 ). In brief, 400 μL plasma sample was added to 1,200 μL ice-methanol solution (4:1 [vol/vol]) and centrifuged at 13,000 × g for 15 min at 4°C.…”

Lacticaseibacillus rhamnosus Probio-M9 may be vertically transmitted from mother to infant during lactation based on faeces metagenomics

“…Lacticaseibacillus rhamnosus Probio-M9 (Probio-M9) is a probiotic independently isolated from the breast milk of a healthy mother in China. Probio-M9 has been shown to maintain the homeostasis of host intestinal microbiota and has good (Zheng et al, 2021). This work aimed to investigate 1) whether Probio-M9 could transmit vertically from mother to infant, and 2) the effects of Probio-M9 consumption on the microecological and microbiota functions of maternal and infant intestines.…”

The Space Environment Activates Capsular Polysaccharide Production in Lacticaseibacillus rhamnosus Probio-M9 by Mutating the wze ( ywqD ) Gene

“…Lacticaseibacillus rhamnosus Probio-M9 was isolated from a breast milk sample from a healthy woman and has good probiotic potential, exhibiting a high growth rate and strong tolerance to bile salts and artificial gastrointestinal fluids ( 19 ). Probio-M9 has shown effective antitumor effects in multiple rodent studies ( 20 , 21 ) and a stress reduction effect in a cohort of graduate students ( 22 ). This study explored the effect of space exposure on Probio-M9, with the aim of inducing stable physiological changes in this bacterial strain.…”