Lactobacillus salivarius UCC118™ Dampens Inflammation and Promotes Microbiota Recovery to Provide Therapeutic Benefit in a DSS-Induced Colitis Model

Iyer, Namrata; Williams, Michelle A.; O’Callaghan, Amy; Dempsey, Elaine; Cabrera‐Rubio, Raúl; Raverdeau, Mathilde; Crispie, Fiona; Cotter, Paul D.; Corr, Sinéad C.

doi:10.3390/microorganisms10071383

Cited by 14 publications

(5 citation statements)

References 34 publications

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“…L. salivarius alleviated inflammation and restored a balanced environment in a DSS-induced colitis model. 74 L. johnsonii has shown efficacy in reducing infection by the diplomonad Giardia intestinalis in gerbils and in inhibiting C. perfringens colonization in chickens. 75 , 76 Studies have indicated that the oral administration of L. delbrueckii regulates mucosal and systemic immune responses in mice with colitis.…”

Section: Resultsmentioning

confidence: 99%

A tailored series of engineered yeasts for the cell-dependent treatment of inflammatory bowel disease by rational butyrate supplementation

Wu,

Huang,

Wang

et al. 2024

Gut Microbes

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Intestinal microbiota dysbiosis and metabolic disruption are considered essential characteristics in inflammatory bowel disorders (IBD). Reasonable butyrate supplementation can help patients regulate intestinal flora structure and promote mucosal repair. Here, to restore microbiota homeostasis and butyrate levels in the patient’s intestines, we modified the genome of Saccharomyces cerevisiae to produce butyrate. We precisely regulated the relevant metabolic pathways to enable the yeast to produce sufficient butyrate in the intestine with uneven oxygen distribution. A series of engineered strains with different butyrate synthesis abilities was constructed to meet the needs of different patients, and the strongest can reach 1.8 g/L title of butyrate. Next, this series of strains was used to co-cultivate with gut microbiota collected from patients with mild-to-moderate ulcerative colitis. After receiving treatment with engineered strains, the gut microbiota and the butyrate content have been regulated to varying degrees depending on the synthetic ability of the strain. The abundance of probiotics such as Bifidobacterium and Lactobacillus increased, while the abundance of harmful bacteria like Candidatus Bacilloplasma decreased. Meanwhile, the series of butyrate-producing yeast significantly improved trinitrobenzene sulfonic acid (TNBS)-induced colitis in mice by restoring butyrate content. Among the series of engineered yeasts, the strain with the second-highest butyrate synthesis ability showed the most significant regulatory and the best therapeutic effect on the gut microbiota from IBD patients and the colitis mouse model. This study confirmed the existence of a therapeutic window for IBD treatment by supplementing butyrate, and it is necessary to restore butyrate levels according to the actual situation of patients to restore intestinal flora.

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Section: Resultsmentioning

confidence: 99%

A tailored series of engineered yeasts for the cell-dependent treatment of inflammatory bowel disease by rational butyrate supplementation

Wu,

Huang,

Wang

et al. 2024

Gut Microbes

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“…Lacticaseibacillus rhamnosus PL60 supplementation increased signals of apoptosis, and uncoupling protein levels in adipose tissue, and serum leptin, which can contribute to reducing obesity. The administration of Ligilactobacillus salivarius UCC118Bac+ (producing bacteriocin) or the bacteriocin-negative strain L. salivarius UCC118Bac− (non-bacteriocin-producing) to rats can modulate the gut microbiota [99]. However, only the bacteriocin-producing probiotics increased the number of Bacteroidetes and Proteobacteria and decreased the number of Actinobacteria compared with the non-bacteriocin-producing control treatments.…”

Section: Discussionmentioning

confidence: 99%

Insights into the Anti-Adipogenic and Anti-Inflammatory Potentialities of Probiotics against Obesity

Kober,

Saha,

Ayyash

et al. 2024

Nutrients

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Functional foods with probiotics are safe and effective dietary supplements to improve overweight and obesity. Thus, altering the intestinal microflora may be an effective approach for controlling or preventing obesity. This review aims to summarize the experimental method used to study probiotics and obesity, and recent advances in probiotics against obesity. In particular, we focused on studies (in vitro and in vivo) that used probiotics to treat obesity and its associated comorbidities. Several in vitro and in vivo (animal and human clinical) studies conducted with different bacterial species/strains have reported that probiotics promote anti-obesity effects by suppressing the differentiation of pre-adipocytes through immune cell activation, maintaining the Th1/Th2 cytokine balance, altering the intestinal microbiota composition, reducing the lipid profile, and regulating energy metabolism. Most studies on probiotics and obesity have shown that probiotics are responsible for a notable reduction in weight gain and body mass index. It also increases the levels of anti-inflammatory adipokines and decreases those of pro-inflammatory adipokines in the blood, which are responsible for the regulation of glucose and fatty acid breakdown. Furthermore, probiotics effectively increase insulin sensitivity and decrease systemic inflammation. Taken together, the intestinal microbiota profile found in overweight individuals can be modified by probiotic supplementation which can create a promising environment for weight loss along enhancing levels of adiponectin and decreasing leptin, tumor necrosis factor (TNF)-α, interleukin (IL)-6, monocyte chemotactic protein (MCP)-1, and transforming growth factor (TGF)-β on human health.

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“…As a potential probiotic, Lactobacillus salivarius ( L. salivarius ) exhibits inhibitory effects on intestinal pathogens and regulates the equilibrium of the gut microbiome by producing various metabolites, antibacterial compounds, antioxidants, and organic acids [ 1 , 2 ]. L. salivarius is also reported to alleviate diseases of the liver by reshaping the gut microbiota, regulating lipid metabolism and inflammation, and promoting lipid degradation [ 3 - 5 ].…”

Section: Introductionmentioning

confidence: 99%

Isolation and Proteomic Analysis of Extracellular Vesicles from Lactobacillus salivarius SNK-6

Huang,

Zhao,

et al. 2023

J. Microbiol. Biotechnol.

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The proteins carried by the extracellular vesicles of Lactobacillus salivarius SNK-6 (LsEVs) were identified to provide a foundation for further explorations of the probiotic activities of L. salivarius SNK-6. LsEVs were isolated from the culture media of L. salivarius SNK-6 and morphological analysis was conducted by scanning electron microscopy. Subsequent transmission electron microscopy and nanoparticle tracking analysis were performed to assess the morphology and particle size of the LsEVs. In addition, the protein composition of LsEVs was analyzed using silver staining and protein mass spectrometry. Finally, internalization of the identified LsEVs was confirmed using a confocal microscope, and enzyme-linked immunosorbent assay was employed to analyze the levels of inflammatory cytokines in LPS-challenged RAW264.7 cells. The results revealed that the membrane-enclosed LsEVs were spherical, with diameters ranging from 100–250 nm. The LsEVs with diameters of 111–256 nm contained the greatest amount of cargo. In total, 320 proteins (10–38 kD) were identified in the LsEVs and included anti-inflammatory molecules, such as PrtP proteinase, co-chaperones, and elongation factor Tu, as well as some proteins involved in glycolysis/gluconeogenesis, such as fructose-1,6-bisphosphate aldolase. Enrichment analysis showed these proteins to be related to the terms “metabolic pathway,” “ribosome,” “glycolysis/gluconeogenesis,” “carbohydrate metabolism,” and “amino acid metabolism.” Furthermore, the LsEVs were internalized by host liver cells and can regulate inflammation. These findings confirm that LsEVs contain various functional proteins that play important roles in energy metabolism, signal transduction, and biosynthesis.

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Lactobacillus salivarius UCC118™ Dampens Inflammation and Promotes Microbiota Recovery to Provide Therapeutic Benefit in a DSS-Induced Colitis Model

Cited by 14 publications

References 34 publications

A tailored series of engineered yeasts for the cell-dependent treatment of inflammatory bowel disease by rational butyrate supplementation

A tailored series of engineered yeasts for the cell-dependent treatment of inflammatory bowel disease by rational butyrate supplementation

Insights into the Anti-Adipogenic and Anti-Inflammatory Potentialities of Probiotics against Obesity

Isolation and Proteomic Analysis of Extracellular Vesicles from Lactobacillus salivarius SNK-6

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