Lactobacillus spp. reduces morphological changes and oxidative stress induced by deoxynivalenol on the intestine and liver of broilers

Souza, Marielen de; Baptista, Ana Angelita Sampaio; Valdiviezo, Milton James Jiménez; Justino, Larissa; Menck-Costa, Maísa Fabiana; Ferraz, Camila R.; Glória, Eduardo Micotti da; Verri, Waldiceu Aparecido; Bracarense, Ana Paula Frederico Rodrigues Loureiro

doi:10.1016/j.toxicon.2020.07.002

Cited by 48 publications

(38 citation statements)

References 54 publications

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“…In vivo and in vitro studies reported lower levels of SOD in serum [83], GSH in the liver of broilers [120], and both parameters in chicken embryo fibroblast DF-1 cells after DON exposure [119]. The ingestion of DON reduced the GSH in liver, jejunum and ileum, the 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) in the jejunum, and the ferric reducing ability (FRAP) in liver of broilers chickens [123]. Oxidative stress data may constitute an effective source of information to know the bird's welfare assessment.…”

Section: Biomarkers Related To Welfare Parametersmentioning

confidence: 98%

“…The exposure of broiler chickens exposed to DON (10 mg/kg feed) resulted in DNA damage in spleen leukocytes or in blood lympho-cytes [76,121,122]. Regarding the parameters associated with lipid peroxidation, it has been reported that DON increased thiobarbituric acid reactive substances (TBARS) in jejunum and malondialdehyde (MDA) in liver, kidney, serum, and jejunum [76,83,120,123]. On the other hand, the oxidative stress is characterized by a reduction in cellular antioxidant levels with a decreased activity of antioxidant enzymes such as superoxidase dismutase (SOD), catalase (CAT) and glutathione-related systems (GSH), including glutathione peroxidase (GPx) [126].…”

Section: Biomarkers Related To Welfare Parametersmentioning

confidence: 99%

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Biomarkers of Deoxynivalenol Toxicity in Chickens with Special Emphasis on Metabolic and Welfare Parameters

Riahi

Pérez-Vendrell

Ramos

et al. 2021

Toxins

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Deoxynivalenol (DON), a trichothecene mycotoxin produced by Fusarium species, is the most widespread mycotoxin in poultry feed worldwide. Long term-exposure from low to moderate DON concentrations can produce alteration in growth performance and impairment of the health status of birds. To evaluate the efficacy of mycotoxin-detoxifying agent alleviating the toxic effects of DON, the most relevant biomarkers of toxicity of DON in chickens should be firstly determined. The specific biomarker of exposure of DON in chickens is DON-3 sulphate found in different biological matrices (plasma and excreta). Regarding the nonspecific biomarkers called also biomarkers of effect, the most relevant ones are the impairment of the productive parameters, the intestinal morphology (reduction of villus height) and the enlargement of the gizzard. Moreover, the biomarkers of effect related to physiology (decrease of blood proteins, triglycerides, hemoglobin, erythrocytes, and lymphocytes and the increase of alanine transaminase (ALT)), immunity (response to common vaccines and release of some proinflammatory cytokines) and welfare status of the birds (such as the increase of Thiobarbituric acid reactive substances (TBARS) and the stress index), has been reported. This review highlights the available information regarding both types of biomarkers of DON toxicity in chickens.

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Section: Biomarkers Related To Welfare Parametersmentioning

confidence: 98%

Section: Biomarkers Related To Welfare Parametersmentioning

confidence: 99%

Biomarkers of Deoxynivalenol Toxicity in Chickens with Special Emphasis on Metabolic and Welfare Parameters

Riahi

Pérez-Vendrell

Ramos

et al. 2021

Toxins

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show abstract

“…A P-value of less than 0.05 was considered statistically signi cant. To determine the degree of lesion, pathological score was monitored as previously described [32]. And the scanning electron microscope (SEM) (Inspect TM , FEI Ltd., USA) of the intestinal villi was conducted as previously described [33].…”

Section: Histopathology and Microscopic Analysis Of The Intestinementioning

confidence: 99%

Preventive Antibiotic Treatment Increases Neonatal Chicks Susceptibility to Salmonella Infection Via Disrupting Gut Microbiota and Linoleic Acid Metabolism

Mei¹,

Ma²,

Zhai³

et al. 2020

Preprint

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Background: Antimicrobial agents have been widely used in animal farms to prevent and treat animal diseases. However, antimicrobial agents may change the bacterial community and increase susceptibility to the pathogenic bacteria infection. Here, we used metagenomic and metabonomic approach to investigate the effects of florfenicol (FFC) pre-treatment on colonization of Salmonella enterica serovar Enteritidis (S. Enteritidis) in intestines of neonatal chicks through analysis of host responses, microbiota and metabolic changes. Results: We observed that FFC pre-treatment significantly increases the level of S. Enteritidis in the cecal contents, spleen and liver and also induces changes to the cecal microbiota and metabolism. Prior to S. Enteritidis infection, FFC significantly reduced the content of Lactobacillus, and significantly affected the linoleic acid metabolism pathway, including significantly reducing the levels of conjugated linoleic acid (CLA), and significantly increasing the abundance of 12,13-EpOME and 12,13-diHOME in cecum. After infection with S. Enteritidis, the abundance of Proteobacteria were significantly increased and the Salmonella-induced intestinal inflammatory responses and intestinal barrier damage were exacerbated. Supplementation with CLA could maintain intestinal integrity, reduce intestinal inflammation, and directly inhibit Salmonella growth to effectively reduce the Salmonella colonization, whereas the 12,13-diHOME through promoting intestinal inflammation and destroying the intestinal barrier function to support the Salmonella infection. Conclusions: Overall, FFC can decrease levels of Lactobacillus and CLA, and elevate cecal 12,13-diHOME concentrations in neonatal chicks and thereby increases susceptibility to Salmonella infection. This study revealed a potential health impact of antibiotics and disturbed gut microbiota and linoleic acid metabolism might be an intestinal health-impairing attribute and may contribute to Salmonella colonization.

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“…A P-value of less than 0.05 was considered statistically signi cant. To determine the degree of lesion, the pathological score was monitored as previously described [32]. SEM (Inspect TM , FEI Ltd., USA) of the intestinal villi was performed as previously described [33].…”

Section: Histopathology and Microscopic Analysis Of The Intestinementioning

confidence: 99%

Preventive Antibiotic Treatment Increases Susceptibility of Neonatal chicks to Salmonella Infection via Disrupting Gut Microbiota and Linoleic Acid Metabolis

Mei¹,

Ma²,

Zhai³

et al. 2020

Preprint

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Background: Antibiotics are widely employed in animal husbandry to prevent and treat diseases. Increasing evidence suggests they may alter the animals’ natural microbiota and increase their susceptibility to pathogen. However, the mechanisms linking the gut microbiota and pathogen colonization in poultry have not yet been full elucidated. Herein, we used metagenomic and metabonomic approaches to investigate the effects of florfenicol (FFC) pre-treatment on Salmonella enterica serovar Enteritidis (S. Enteritidis) colonization in the intestines of neonatal chicks in terms of host response, microbiota composition and metabolism. Results: We determined that FFC pre-treatment significantly alters the cecal microbiota and metabolome, and also increases the intestinal permeability and promotes a pro-inflammatory gene expression profile in the host. Host physiological changes were concordant with significantly increased susceptibility to S. Enteritidis infection in chicks with FFC pre-treatment relative to without pre-treatment chicks. Prior to Salmonella infection, FFC pre-treatment significantly reduced the abundance of Lactobacillus, and significantly affected linoleic acid metabolism, including significantly reducing the levels of conjugated linoleic acid (CLA), and significantly elevating the abundance of 12,13-EpOME and 12,13-diHOME in cecum. After infection with S. Enteritidis, the abundance of Proteobacteria were significantly increased and host inflammatory responses and intestinal permeability were significantly aggravated relative to without FFC pre-treatment chicks, suggestive of a profound exacerbating of the host response influenced by infection in the context of FFC pre-treatment. The linoleic acid metabolism was still significantly different pathway after Salmonella infection, and we screened CLA and 12,13-diHOME as the target metabolites using a multi-omics technique. Supplementation with CLA maintained intestinal integrity, reduced intestinal inflammation, and accelerated Salmonella clearance from the gut and remission of enteropathy. Whereas, treatment with 12,13-diHOME promoted intestinal inflammation and disrupted the intestinal barrier function to sustain Salmonella infection. Therefore, florfenicol reduces production of CLA by inhibiting Lactobacillus growth, increases 12,13-diHOME level of intestine, thereby reducing colonization resistance of neonatal chicks to Salmonella infection.Conclusion: This study reveals the potential health impact of antibiotics on gut microbiota and linoleic acid metabolism and contributing factors influencing Salmonella colonization in neonatal chicks, and provides mechanistic understanding into the role of the antibiotics promote the colonization of pathogens.

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Lactobacillus spp. reduces morphological changes and oxidative stress induced by deoxynivalenol on the intestine and liver of broilers

Cited by 48 publications

References 54 publications

Biomarkers of Deoxynivalenol Toxicity in Chickens with Special Emphasis on Metabolic and Welfare Parameters

Biomarkers of Deoxynivalenol Toxicity in Chickens with Special Emphasis on Metabolic and Welfare Parameters

Preventive Antibiotic Treatment Increases Neonatal Chicks Susceptibility to Salmonella Infection Via Disrupting Gut Microbiota and Linoleic Acid Metabolism

Preventive Antibiotic Treatment Increases Susceptibility of Neonatal chicks to Salmonella Infection via Disrupting Gut Microbiota and Linoleic Acid Metabolis

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Lactobacillus spp. reduces morphological changes and oxidative stress induced by deoxynivalenol on the intestine and liver of broilers

Cited by 48 publications

References 54 publications

Biomarkers of Deoxynivalenol Toxicity in Chickens with Special Emphasis on Metabolic and Welfare Parameters

Biomarkers of Deoxynivalenol Toxicity in Chickens with Special Emphasis on Metabolic and Welfare Parameters

Preventive Antibiotic Treatment Increases Neonatal Chicks Susceptibility to Salmonella Infection Via Disrupting Gut Microbiota and Linoleic Acid Metabolism

Preventive&nbsp;Antibiotic&nbsp;Treatment&nbsp;Increases&nbsp;Susceptibility&nbsp;of&nbsp;Neonatal&nbsp;chicks&nbsp;to&nbsp;Salmonella&nbsp;Infection&nbsp;via&nbsp;Disrupting&nbsp;Gut&nbsp;Microbiota&nbsp;and&nbsp;Linoleic&nbsp;Acid&nbsp;Metabolis

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Preventive Antibiotic Treatment Increases Susceptibility of Neonatal chicks to Salmonella Infection via Disrupting Gut Microbiota and Linoleic Acid Metabolis