Lactococcus lactis, a bacterium with probiotic functions and pathogenicity

Wu, Fan; Xie, Xinmei; Du, Tao; Jiang, Xiaodan; Miao, Wei; Wang, Tiancheng

doi:10.1007/s11274-023-03771-5

Cited by 16 publications

(3 citation statements)

References 118 publications

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“…cremoris is receiving a lot of attention from researchers, and consequently a series of molecular genetic tools have been developed, among which the promoter, as one of the important components of gene expression, closely affects gene expression, and there is an urgent need for expanding the L. cremoris promoter library, especially for screening endogenous constitutive promoters. − …”

Section: Discussionmentioning

confidence: 99%

Characterization of a Panel of Constitutive Promoters from Lactococcus cremoris for Fine-Tuning Gene Expression

Wang,

Ai,

Xia

et al. 2024

ACS Synth. Biol.

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Lactococcus cremoris (homotypic synonym: Lactococcus lactis) is receiving increasing attention as a prominent vehicle for the delivery of live vaccines. This can hardly be achieved without developing tools for the genetic manipulation of L. cremoris, and the paucity of studies on L. cremoris endogenous promoters has attracted our attention. Here, we report the discovery and characterization of 29 candidate promoters identified from L. cremoris subsp. cremoris NZ9000 by RNA sequencing analysis. Furthermore, 18 possible constitutive promoters were obtained by RT-qPCR screening from these 29 candidate promoters. Then, these 18 promoters were cloned and characterized by a reporter gene, gusA, encoding β-glucuronidase. Eventually, eight endogenous constitutive promoters of L. cremoris were obtained, which can be applied to genetic manipulation of lactic acid bacteria.

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Section: Discussionmentioning

confidence: 99%

Characterization of a Panel of Constitutive Promoters from Lactococcus cremoris for Fine-Tuning Gene Expression

Wang,

Ai,

Xia

et al. 2024

ACS Synth. Biol.

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“…Many bacteria associated with vertebrate hosts have been found in cases of opportunistic infection. Even for species seemingly suitable for probiotic use, cases of pathogenicity have been described, for example for L. lactis in cows (57), and even in humans (58). Even in commercially available probiotics there are many unknowns, and some risks associated with their use.…”

Section: Following Legislation Might Be Too Stringent For Selecting P...mentioning

confidence: 99%

Targeted isolation of bacteria with potential to competitively excludeStaphylococcus aureusin the upper respiratory tract of pigs

Vlasblom,

Patel,

Lawlor

et al. 2024

Preprint

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Considering global antimicrobial resistance (AMR) prevalence, alternative or complementary strategies to antimicrobial use, are of interest. Livestock-associated methicillin-resistantStaphylococcus aureus(LA-MRSA) is of particular interest as despite significant AMU reduction, LA-MRSA prevalence in pig husbandry has not decreased. We performed targeted isolation of bacterial species with potential antagonism against LA-MRSA in pig farms. Duplicate piglet nasal swabs from three European countries (Germany, Ireland and The Netherlands) were taken longitudinally from birth up to 10 weeks, one for amplicon sequencing and qPCR, and the other was cryopreserved for culturing. We identified potential probiotic species by anticorrelation analysis of bacterial abundance from amplicon sequencing data with quantitativeS. aureusestimates from qPCR data from the samples. A literature-screen was performed on the species identified, to determine their probiotic potential. Following this, 1302 isolates were grown from selected cryopreserved swabs and identified using MALDI-TOF and additional 16S rRNA gene sequencing to isolate the anticorrelating species. Ninety-five isolates of interest were screened for absence of tetracycline resistance and hemolytic activity and whole genome sequencing was conducted to verify their taxonomy and to assess their AMR and virulence gene profile. Additional phenotypic antimicrobial resistance testing selected three differentLactococcus lactisstrains. During anin vitrochallenge using spent medium, all three strains demonstrated inhibition against twoS. aureusstrains. TheseL. lactisstrains may have the ability to be used safely to reduce LA-MRSA carriage in the nasal passages of pigs but furtherin vivotesting is necessary to confirm this potential.ImportanceAn approach to tackle antimicrobial resistance in livestock is to use competitive exclusion agents. We employed a sequencing guided isolation approach to identify bacterial species within silicoantagonism to livestock-associatedStaphylococcus aureus(LA-MRSA) in the nasal microbiome of piglets. Based on this, threeLactococcus lactisisolates were found to be suitable for further probiotic testing. This strategy can be used to effectively identify, isolate, and screen bacteria that live in antagonism with opportunistic pathogens such as LA-MRSA in a complex microbiome.

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“…In the past couple of years, several literature reviews concerning L. lactis were published. Several works reviewed probiotic characteristics of L. lactis [16,17]. A review by Li et al [2] aimed to investigate fermentation characteristics of L. lactis subsp.…”

Section: Introductionmentioning

confidence: 99%

Lactococcus lactis in Dairy Fermentation—Health-Promoting and Probiotic Properties

Kondrotiene,

Zavistanaviciute,

Aksomaitiene

et al. 2023

Fermentation

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The use of lactic acid bacteria (LAB) in the fermentation process to produce fermented foods has a long history. Furthermore, LAB are beneficial microorganisms known for their health-promoting characteristics. During fermentation, LAB have the capacity to produce significant amounts of bioactive substances, such as peptides, bacteriocins, lactic acid, exopolysaccharides (EPSs), enzymes, and others. Lactococcus lactis as one of the best-known and well-characterized species of LAB serves as a model organism for studying LAB. For a very long time, L. lactis has been used in milk fermentation, both in well-monitored industrial settings and on a small scale in traditional operations. Furthermore, L. lactis is a vital microorganism in the dairy food fermentation industry due to its role in acidification, flavor development, and the creation of various dairy products, including cheese, fermented butter, and others. The novelty of this review is the comprehensive and organized presentation of the main benefits of the use of L. lactis in milk fermentation processes including technological and safety features relevant for the dairy industry, probiotic potential, the ability to produce bioactive compounds (e.g., bacteriocins, GABA), and the recent development of such bacteria research methods like whole genome sequencing (WGS).

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Lactococcus lactis, a bacterium with probiotic functions and pathogenicity

Cited by 16 publications

References 118 publications

Characterization of a Panel of Constitutive Promoters from Lactococcus cremoris for Fine-Tuning Gene Expression

Characterization of a Panel of Constitutive Promoters from Lactococcus cremoris for Fine-Tuning Gene Expression

Targeted isolation of bacteria with potential to competitively excludeStaphylococcus aureusin the upper respiratory tract of pigs

Lactococcus lactis in Dairy Fermentation—Health-Promoting and Probiotic Properties

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