Lactoferricin, an antimicrobial motif derived from lactoferrin with food preservation potential

Wu, Jiajia; Zang, Mingwu; Wang, Shouwei; Qiao, Xianliang; Zhao, Bing; Bai, Jing; Zhao, Yan; Shi, Yuxuan

doi:10.1080/10408398.2023.2207650

Cited by 6 publications

(3 citation statements)

References 49 publications

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“…The study suggests that BLF can be utilized as a natural antimicrobial agent in cold liquid food systems [ 49 ]. Furthermore, a recent review has summarized the potential of using LFcin-related peptides against food pathogens and suggests the utilization of LFcin for food preservation [ 50 ].…”

Section: The Potential Prebiotic Activity Of Lactoferrin In Vitromentioning

confidence: 99%

Featured Prebiotic Agent: The Roles and Mechanisms of Direct and Indirect Prebiotic Activities of Lactoferrin and Its Application in Disease Control

Liu

Chen

2023

Nutrients

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Lactoferrin (LF) is a glycoprotein found in mammalian milk, and lactoferricin is a peptide derived from LF hydrolysate. Both LF and lactoferricin (LFcin) have diverse functions that could benefit mammals. Bovine LF (BLF) and BLFcin exhibit a wide range of antimicrobial activities, but most probiotic strains are relatively resistant to their antibacterial effects. BLF and BLF hydrolysate can promote the growth of specific probiotics depending on the culture conditions, the dose of BLF or BLF-related peptides, and the probiotic strains used. BLF supplementation has been shown to modulate several central molecular pathways or genes in Lacticaseibacillus rhamnosus GG under cold conditions, which may explain the prebiotic roles of BLF. LF alone or in combination with selected probiotics can help control bacterial infections or metabolic disorders, both in animal studies and in human clinical trials. Various LF-expressing probiotics, including those expressing BLF, human LF, or porcine LF, have been developed to facilitate the combination of LFs with specific probiotics. Supplementation with LF-expressing probiotics has positive effects in animal studies. Interestingly, inactivated LF-expressing probiotics significantly improved diet-induced nonalcoholic fatty liver disease (NAFLD) in a mouse model. This review highlights the accumulated evidence supporting the use of LF in combination with selected LF-resistant probiotics or LF-expressing probiotics in the field.

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Section: The Potential Prebiotic Activity Of Lactoferrin In Vitromentioning

confidence: 99%

Featured Prebiotic Agent: The Roles and Mechanisms of Direct and Indirect Prebiotic Activities of Lactoferrin and Its Application in Disease Control

Liu

Chen

2023

Nutrients

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“…Our data, obtained from fluoroquinolones conjugated to TP10 [8,16], as well as chimera peptides composed of human neutrophil peptide (HNP) [43], demonstrate selectivity against both microbes and leukemic cells, as opposed to healthy human cell cultures. To investigate the anticancer efficacy of a PP other than CPPs (TP10 and NHP) combined with antibiotics, we selected lactoffericin B (LfcinB), an AMP with established anticancer activity and low toxicity [47][48][49][50][51][52][53][54]. LfcinB is a shorter version of lactoferrin, and like the original peptide, it demonstrates anticancer properties [55][56][57][58][59][60][61][62].…”

Section: Introductionmentioning

confidence: 99%

Lactoferricin B Combined with Antibiotics Exhibits Leukemic Selectivity and Antimicrobial Activity

Lica,

Gucwa,

Heldt

et al. 2024

Molecules

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The fusion of penetrating peptides (PPs), e.g., cell penetration peptides (CPPs) or antimicrobial peptides (AMPs), together with antimicrobial agents is an expanding research field. Specific AMPs, such as lactoferricin B (LfcinB), have demonstrated strong antibacterial, antifungal, and antiparasitic activity, as well as valuable anticancer activity, proving beneficial in the development of anticancer conjugates. The resulting conjugates offer potential dual functionality, acting as both an anticancer and an antimicrobial agent. This is especially necessary in cancer treatment, where microbial infections pose a critical risk. Leukemic cells frequently exhibit altered outer lipid membranes compared to healthy cells, making them more sensitive to compounds that interfere with their membrane. In this study, we revisited and reanalyzed our earlier research on LfcinB and its conjugates. Furthermore, we carried out new experiments with a specific focus on cell proliferation, changes in membrane asymmetric phosphatidylserine location, intracellular reactive oxygen species (ROS) generation, mitochondrial functions, and in vitro bacterial topoisomerase inhibition.

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“…It is most abundant in human and bovine colostrum and milk (Cheng et al, 2008), but it can also be found in further body fluids such as tears, saliva, plasma and semen, as well as in the gastrointestinal, respiratory and reproductive tracts (Superti, 2020). Lactoferrin and its derivatives possess immunomodulatory, antiinflammatory and antioxidant activity, as well as antibacterial, antifungal, antiviral, antiparasitic, antitumour and antianaemic properties (Sienkiewicz et al, 2022;Wu et al, 2023). Furthermore, the molecule can have a positive impact on oral as well as intestinal microbiota (Nakano et al, 2017;Haiwen et al, 2019;Superti, 2020).…”

Section: Introductionmentioning

confidence: 99%

Lactoferrin-based food supplements trigger toxin production of enteropathogenic Bacillus cereus

Jugert,

Didier,

Jessberger

2023

Front. Microbiol.

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Lactoferrin is an iron-binding glycoprotein exhibiting antibacterial, antiviral, antifungal, antiparasitic, antiinflammatory, antianaemic and anticarcinogenic properties. While its inhibitory effects against bacterial pathogens are well investigated, little is known about its influence on the production and/or mode of action of bacterial toxins. Thus, the present study aimed to determine the impact of food supplements based on bovine lactoferrin on Bacillus cereus enterotoxin production. First, strain-specific growth inhibition of three representative isolates was observed in minimal medium with 1 or 10 mg/mL of a lactoferrin-based food supplement, designated as product no. 1. Growth inhibition did not result from iron deficiency. In contrast to that, all three strains showed increased amounts of enterotoxin component NheB in the supernatant, which corresponded with cytotoxicity. Moreover, lactoferrin product no. 1 enhanced NheB production of further 20 out of 28 B. cereus and Bacillus thuringiensis strains. These findings again suggested a strain-specific response toward lactoferrin. Product-specific differences also became apparent comparing the influence of further six products on highly responsive strain INRA C3. Highest toxin titres were detected after exposure to products no. 7, 1 and 2, containing no ingredients except pure bovine lactoferrin. INRA C3 was also used to determine the transcriptional response toward lactoferrin exposure via RNA sequencing. As control, iron-free medium was also included, which resulted in down-regulation of eight genes, mainly involved in amino acid metabolism, and in up-regulation of 52 genes, mainly involved in iron transport, uptake and utilization. In contrast to that, 153 genes were down-regulated in the presence of lactoferrin, including genes involved in flagellar assembly, motility, chemotaxis and sporulation as well as genes encoding regulatory proteins, transporters, heat and cold shock proteins and virulence factors. Furthermore, 125 genes were up-regulated in the presence of lactoferrin, comprising genes involved in sporulation and germination, nutrient uptake, iron transport and utilization, and resistance. In summary, lactoferrin exposure of B. cereus strain-specifically triggers an extensive transcriptional response that considerably exceeds the response toward iron deficiency and, despite down-regulation of various genes belonging to the PlcR-regulon, ultimately leads to an increased level of secreted enterotoxin by a mechanism, which has yet to be elucidated.

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Lactoferricin, an antimicrobial motif derived from lactoferrin with food preservation potential

Cited by 6 publications

References 49 publications

Featured Prebiotic Agent: The Roles and Mechanisms of Direct and Indirect Prebiotic Activities of Lactoferrin and Its Application in Disease Control

Featured Prebiotic Agent: The Roles and Mechanisms of Direct and Indirect Prebiotic Activities of Lactoferrin and Its Application in Disease Control

Lactoferricin B Combined with Antibiotics Exhibits Leukemic Selectivity and Antimicrobial Activity

Lactoferrin-based food supplements trigger toxin production of enteropathogenic Bacillus cereus

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