Lactoferrin Enhanced Apoptosis and Protected Against Thioacetamide-Induced Liver Fibrosis in Rats

Hessin, Alyaa F.; Hegazy, Rehab; Hassan, Azza; Yassin, Nemat A.; Kenawy, Sanaa A.

doi:10.3889/oamjms.2015.038

Cited by 43 publications

(34 citation statements)

References 57 publications

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“…Severe fibrous connective tissue proliferation forming fibrous bridges connecting the portal areas led toformation of pseudolobulation which separate the hepatic lobe from other lobules. These finding were agreed with the results of Yeh et al, (2004) and Hessin et al, (2015). Meanwhile, after MSCs treatment there was thin strands of fibrous connective tissue in between the hepatic lobules.…”

Section: Discussionsupporting

confidence: 93%

Experimental pathological studies on the ability of Stem cells for regeneration of hepatocytes in rats

Gab-Allah

El-Mashad

Ahmed³

et al. 2017

Benha Veterinary Medical Journal

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A B S T R A C TStem cell therapy is an important catigory of regenerative medicine has the potential to provide a valuable adjunct and alternative to liver transplantation with treatment of liver fibrosis and cirrhosis. In this approch, 70 male albino rats were randomly divided into 4 groups: Group 1: Control group (20 rats), Group 2: TAA-treated group (20 rats), Group 3: Mesenchymal stem cells treated group (15 rats) and Group 4: TAA-Mesenchymal stem cell -treated group (15 rats). Our results showed that TAA is significantly increase the activities of ALT, AST, and ALP and reduced the levels of total protein, albumin and globulins in the serum. Moreover, TAA successfully induced liver cirrhosis characterized by coarse nodular liver surface with severe fibrous connective tissue proliferation led to pseudolobulation of hepatic parenchyma. Meanwhile, after treatment with Mesenchymal stem cells (MSCs) the liver of the rats was grossly nearly normal and microscopically thin strands of fibrous connective tissue in between the hepatic lobules with mild degenerative and necrotic changes and significant improvement in the liver blood parameters. These findings concluded that MSCs have ameliorative effect on chemically induced liver cirrhosis through their regenerative capacity and antifibrotic effect.

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Section: Discussionsupporting

confidence: 93%

Experimental pathological studies on the ability of Stem cells for regeneration of hepatocytes in rats

Gab-Allah

El-Mashad

Ahmed³

et al. 2017

Benha Veterinary Medical Journal

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“…These results were in agreement with (Salama et al 2013, Hessin et al 2015. Hypoproteinemia, observed in this study perhaps occur due to inflammatory reaction or might be due to perturbed protein biosynthesis in the cirrhotic liver as demonstrated by (Alshawsh et al 2011).…”

Section: Discussionsupporting

confidence: 91%

Antioxidant and Hepatoprotective Effects of Tranilast against Thioacetamide-Induced Liver Fibrosis in Rats

Ramadan

Afifi

Yassin

et al. 2017

Journal of Applied Veterinary Sciences

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The aim of the present study was to investigate the potential antioxidant and Hepatoprotective activities of tranilast against TAA-induced liver fibrosis in rats. Methods: Liver fibrosis was induced in rats by intra peritoneal injection of TAA (200 mg/kg) for 6 weeks. Tranilast was administered orally at a dose of 150 and 300mg/kg for 6 weeks before induction of liver fibrosis. Results: Injection of TAA induced hepatic fibrosis that was manifested by a significant increase in the activities of aminotransferases and total bilirubin with a significant decrease in total protein and albumin in serum. Liver homogenate of TAA rats had the lower content of reduced glutathione (GSH) and superoxide dismutase (SOD) with increased levels of the hepatic malondialdehyde. Histological data presented marked damage in liver sections of TAA rats. Oral dosing of tranilast for 6 weeks before induction of liver fibrosis reversed these altered parameters near to normal values. These results suggest that tranilast could afford significant protection and antioxidant effect in prevention of liver fibrosis. ‫ـــــــــــــــــــــــــــــــــــــــــ‬

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“…The improvement of liver nonenzymic antioxidants shown in the current study in the DEN‐treated mice by WPC may be due to its sulfur amino acids that enhanced the production of GSH. Also, lactoferrin had the ability to reduce the oxidative stress in thioacetamide‐induced liver fibrosis and 4‐nitroquinoline 1‐oxide‐induced tongue carcinogenesis in rats by activating the detoxifying enzymes . Moreover, the iron‐binding capability of lactoferrin protected from tissue injury caused by iron‐induced ROS, such as superoxide anions and hydroxyl radicals …”

Section: Discussionmentioning

confidence: 99%

“…Also, lactoferrin had the ability to reduce the oxidative stress in thioacetamide-induced liver fibrosis and 4-nitroquinoline 1-oxide-induced tongue carcinogenesis in rats by activating the detoxifying enzymes. 32,33 Moreover, the ironbinding capability of lactoferrin protected from tissue injury caused by iron-induced ROS, such as superoxide anions and hydroxyl radicals. 34 The DEN-induced hepatocarcinogenic mice showed a significant increase in the markers of liver carcinogenesis and systemic inflammation (serum α-fetoprotein and TNF-α, respectively), as well as hepatic DNA fragmentation and apoptosis (active caspases 3, 8, and 9), compared with the noncancerous mice.…”

Section: Discussionmentioning

confidence: 99%

“…15,16 Therefore, the present study aimed to evaluate and compare the antihepatocarcinogenic activity of different doses of bovine WPC and its bioactive component "lactoferrin" in the DEN-treated male albino mice, as well as monitoring some mechanisms of their action with special reference to the liver antioxidant defense system and the inflammatory/apoptotic pathway. 32.47 g WPC-80 yield 25 g of complete protein) was purchased from NutraBio Labs (Middlesex, New Jersey). It is free from pathogens and contains 82.3% dry basis protein, 6.2% fat, 2.5% ash, <7.5% scorched particle, and 5.1% moisture.…”

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confidence: 99%

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Antihepatocarcinogenic activity of whey protein concentrate and lactoferrin in diethylnitrosamine‐treated male albino mice

Mohammed

Ramadan

Zoheiry

et al. 2019

Environmental Toxicology

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Hepatocellular carcinoma is considered one of the most prevalent and lethal malignancies worldwide. Chemotherapy with cytotoxic agents showed a low response rate with possible toxic effects. Recently, some emphases have been placed on the anticancer properties of bovine whey protein and its components, especially lactoferrin. The present study aimed to evaluate and compare the antihepatocarcinogenic activity of bovine whey protein concentrate (WPC, 300 and 600 mg/kg body weight) and lactoferrin (30 and 60 mg/kg body weight), orally and daily for 14 weeks, in the mice model of diethylnitrosamine (DEN)‐induced hepatocarcinogenesis. The results showed that both WPC and lactoferrin (in a dose‐dependent manner) alleviated significantly (P < .001) the elevation in serum markers of liver carcinoma and inflammation in the DEN‐treated mice. Also, they exhibited a great amelioration in the livers' histological structure of the DEN‐treated mice by 37.0% to 66.7%. In addition, they decreased significantly (P < .001) the hepatic DNA fragmentation in the DEN‐treated mice by 23.1% to 32.7%. Only, the high doses of WPC and lactoferrin completely modulated the decrease in the activity of liver enzymic antioxidant defense system (catalase, glutathione peroxidase, and superoxide dismutase) and improved significantly (P < .01‐.001) the concentration of hepatic reduced glutathione of the DEN‐treated mice. Moreover, the high doses of WPC and lactoferrin reduced significantly (P < .05‐.001) the elevation in the concentrations of hepatic active caspases 3, 8, and 9 of the DEN‐treated mice. In conclusion, both WPC and lactoferrin were effective in inhibiting the hepatocarcinogenic activity of DEN in mice model through their ability to alleviate the hepatic inflammation and apoptosis.

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Lactoferrin Enhanced Apoptosis and Protected Against Thioacetamide-Induced Liver Fibrosis in Rats

Cited by 43 publications

References 57 publications

Experimental pathological studies on the ability of Stem cells for regeneration of hepatocytes in rats

Experimental pathological studies on the ability of Stem cells for regeneration of hepatocytes in rats

Antioxidant and Hepatoprotective Effects of Tranilast against Thioacetamide-Induced Liver Fibrosis in Rats

Antihepatocarcinogenic activity of whey protein concentrate and lactoferrin in diethylnitrosamine‐treated male albino mice

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