Lactoperoxidase and Histatin 5 – their Adsorption Behaviour on Silica and Hydrophobized Silica Surfaces, and Implications on their Role in the Initial Salivary Film Formation

Svendsen, Ida; Lindh, Liselott; Arnebrant, Thomas

doi:10.1524/zpch.2007.221.1.65

Cited by 2 publications

(1 citation statement)

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“…It is known that LPO interacts with membranes through hydrophobic interactions 40. 41 Therefore, to check whether LPO is attached at the vesicles surface we incubated a solution of empty vesicles with Alexa633‐LPO (30 n M ) and compared it with a solution of Alexa633‐LPO‐containing vesicles. In the case of empty vesicles, large clusters with diffusion times of up to 30 ms were formed, which revealed that the interaction of Alexa633‐LPO with the vesicle surface induces aggregation.…”

Section: Resultsmentioning

confidence: 99%

Enzymatic Cascade Reactions inside Polymeric Nanocontainers: A Means to Combat Oxidative Stress

Tanner

Onaca

Balasubramanian

et al. 2011

Chemistry A European J

132

166

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Oxidative stress, which is primarily due to an imbalance in reactive oxygen species, such as superoxide radicals, peroxynitrite, or hydrogen peroxide, represents a significant initiator in pathological conditions that range from arthritis to cancer. Herein we introduce the concept of enzymatic cascade reactions inside polymeric nanocontainers as an effective means to detect and combat superoxide radicals. By simultaneously encapsulating a set of enzymes that act in tandem inside the cavities of polymeric nanovesicles and by reconstituting channel proteins in their membranes, an efficient catalytic system was formed, as demonstrated by fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy. Superoxide dismutase and lactoperoxidase were selected as a model to highlight the combination of enzymes. These were shown to participate in sequential reactions in situ in the nanovesicle cavity, transforming superoxide radicals to molecular oxygen and water and, therefore, mimicking their natural behavior. A channel protein, outer membrane protein F, facilitated the diffusion of lactoperoxidase substrate/products and dramatically increased the penetration of superoxide radicals through the polymer membrane, as established by activity assays. The system remained active after uptake by THP-1 cells, thus behaving as an artificial organelle and exemplifying an effective approach to enzyme therapy.

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Section: Resultsmentioning

confidence: 99%