“…κ-CN was detected by HPLC in higher concentrations compared to SDS-PAGE, where it was apparently the least abundant protein. κ-CN may undergo thiol disulfide linkages with proteins containing disulfide bonds, such as α-La, β-Lg, BSA, IGs and Lf, when subjected to high temperatures, resulting in ҡ-CN aggregates that move slowly or remain stacked in the stacking gel during the electrophoresis run, altering correct concentration estimations [59]. Consequently, the caseins and whey protein contents detected in the investigated IFs are not in agreement with public health authority recommendations and are dissimilar to human milk composition, where whey proteins should be present in higher amounts compared to caseins, as they are better digested due to coagulation in the acidic stomach environment, and consequently, delay the release of free amino acids [60].…”