Lake Cadagno: Microbial Life in Crenogenic Meromixis

Tonolla, Mauro; Storelli, Nicola; Danza, Francesco; Ravasi, Damiana; Peduzzi, Sandro; Posth, Nicole R.; Cox, Raymond P.; Jørgensen, Mårten Flø; Gregersen, Lea H.; Daugbjerg, Niels; Frigaard, Niels‐Ulrik

doi:10.1007/978-3-319-49143-1_7

Cited by 23 publications

(31 citation statements)

References 91 publications

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“…The δ 13 C POC measured in the Cadagno chemocline in late June and early October were −41‰ and −39‰, respectively (Figure 4b, Table S1A). The ∆ 13 C value at Lake Cadagno is higher than, but well within range of that estimated in the chemocline in other sulfidic, meromictic lakes, and fjords with strong populations of anoxygenic phototrophs, even in cases where PSB dominated the chemocline (Bovee & Pearson, 2014;Pimenov, Lunina, Prusakova, Rusanov, & Ivanov, 2008;Velinsky & Fogel, 1999;Zyakun et al, 2009 (Peduzzi et al, 2012;Tonolla, Peduzzi, Demarta, Peduzzi, & Hahn, 2004;Tonolla et al, 1999Tonolla et al, , 2017. At the chemocline, however, C-isotope fractionation increases, reaching a mid-chemocline value of 32‰.…”

Section: Carbon Isotope Fractionation Due To C-fixation In the Chemsupporting

confidence: 73%

“…At the chemocline, however, C-isotope fractionation increases, reaching a mid-chemocline value of 32‰. okenii is a large cell, up to 7 μm in length and 5 μm in width (Tonolla et al, 2017). The PSB Chr.…”

Section: Carbon Isotope Fractionation Due To C-fixation In the Chemmentioning

confidence: 99%

“…Purpureus, and Tdc. syntrophicum (Peduzzi et al, 2012;Tonolla, Peduzzi, Demarta, Peduzzi, & Hahn, 2004;Tonolla et al, 1999Tonolla et al, , 2017. The PSB Chr.…”

Section: Carbon Isotope Fractionation Due To C-fixation In the Chemmentioning

confidence: 99%

“…Chr. okenii is a large cell, up to 7 μm in length and 5 μm in width (Tonolla et al, 2017). Indeed, when C-uptake was normalized to cell volume, Chr.…”

Section: Carbon Isotope Fractionation Due To C-fixation In the Chemmentioning

confidence: 99%

“…This small, alpine lake (830 × 420 m, 21 m depth) lies within Pre-Triassic gneiss and mica-schists and along a contact of the Piora-syncline characterized by dolomitic limestones and gypsum (Tonolla et al, 2017). This small, alpine lake (830 × 420 m, 21 m depth) lies within Pre-Triassic gneiss and mica-schists and along a contact of the Piora-syncline characterized by dolomitic limestones and gypsum (Tonolla et al, 2017).…”

mentioning

confidence: 97%

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Carbon isotope fractionation by anoxygenic phototrophic bacteria in euxinic Lake Cadagno

et al. 2017

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Anoxygenic phototrophic bacteria utilize ancient metabolic pathways to link sulfur and iron metabolism to the reduction of CO . In meromictic Lake Cadagno, Switzerland, both purple sulfur (PSB) and green sulfur anoxygenic phototrophic bacteria (GSB) dominate the chemocline community and drive the sulfur cycle. PSB and GSB fix carbon utilizing different enzymatic pathways and these fractionate C-isotopes to different extents. Here, these differences in C-isotope fractionation are used to constrain the relative input of various anoxygenic phototrophs to the bulk community C-isotope signal in the chemocline. We sought to determine whether a distinct isotopic signature of GSB and PSB in the chemocline persists in the settling fraction and in the sediment. To answer these questions, we also sought investigated C-isotope fractionation in the water column, settling material, and sediment of Lake Cadagno, compared these values to C-isotope fractionation of isolated anoxygenic phototroph cultures, and took a mass balance approach to investigate relative contributions to the bulk fractionation signature. We found a large C-isotope fractionation between dissolved inorganic carbon (DIC) and particulate organic carbon (POC) in the Lake Cadagno chemocline. This large fractionation between the DIC and POC was also found in culture experiments carried out with anoxygenic phototrophic bacteria isolated from the lake. In the Lake Cadagno chemocline, anoxygenic phototrophic bacteria controlled the bulk C-isotope fractionation, but the influence of GSB and PSB differed with season. Furthermore, the contribution of PSB and GSB to bulk C-isotope fractionation in the chemocline could be traced in the settling fraction and in the sediment. Taken together with other studies, such as lipid biomarker analyzes and investigations of other stratified lakes, these results offer a firmer understanding of diagenetic influences on bacterial biomass.

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Section: Carbon Isotope Fractionation Due To C-fixation In the Chemsupporting

confidence: 73%

Section: Carbon Isotope Fractionation Due To C-fixation In the Chemmentioning

confidence: 99%

“…Purpureus, and Tdc. syntrophicum (Peduzzi et al, 2012;Tonolla, Peduzzi, Demarta, Peduzzi, & Hahn, 2004;Tonolla et al, 1999Tonolla et al, , 2017. The PSB Chr.…”

Section: Carbon Isotope Fractionation Due To C-fixation In the Chemmentioning

confidence: 99%

“…Chr. okenii is a large cell, up to 7 μm in length and 5 μm in width (Tonolla et al, 2017). Indeed, when C-uptake was normalized to cell volume, Chr.…”

Section: Carbon Isotope Fractionation Due To C-fixation In the Chemmentioning

confidence: 99%

mentioning

confidence: 97%

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Carbon isotope fractionation by anoxygenic phototrophic bacteria in euxinic Lake Cadagno

et al. 2017

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Vertical niche occupation and potential metabolic interplay of microbial consortia in a deeply stratified meromictic model lake

Cabello‐Yeves,

Picazo,

Roda‐Garcia

et al. 2023

Limnology & Oceanography

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The microbial ecology of meromictic lakes assessed with “omics” is still poorly studied compared to other aquatic systems. Here, a combination of metagenomics, high resolution sampling and detailed physical–chemical data gathering allowed to study the planktonic prokaryotic assemblages and metabolic capabilities in the crenogenic meromictic Lake El Tobar (Spain), a model lake for such purposes. This system presents a specific stratification comprising a freshwater layer and a halocline linked to the oxycline, driving to the euxinic hypersaline waters of the deep monimolimnion. The different strata showed a highly diverse and vertically distributed microbiome with their metabolic capacities fitting/influencing the physical–chemical environment. Overall, up to 338 novel genomes were found from metagenome assembled genomes. Picocyanobacteria and methanotrophs were abundant in the upper part of the oxycline. Anoxygenic phototrophs (Chlorobium, Thiohalocapsa, Chromatiaceae, Rhodospirillum, and Rhodobacteraceae spp.) dominated the 12.5–14 m anoxic waters with dim light availability. Sulfate reducers (Desulfobacterota and Firmicutes) inhabited low redox horizons from 13.5 to the bottom (18 m). The potential microbial synergistic performance increases toward the monimolimnion. Among these, a microbial assemblage mostly composed of Spirochaetota, Cloacimonadota, Omitrophota, Firmicutes, Marinisomatota, Nanoarchaeota, and Patescibacteria in hypersaline waters of 14–18 m (conductivities of 118–213 mS cm−1), is potentially capable of performing mixed‐acid fermentations, even including hydrogen and butanol biosynthesis of biotechnological interest. This metagenomics study shows how microbial lifestyles may be determinant in the interplay of environmental gradients, and exemplifies the potential interactions between the microbial guilds thereby.

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Vertical structure of the bacterial diversity in meromictic Fayetteville Green Lake

et al. 2021

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The permanently stratified water columns in euxinic meromictic lakes produce niche environments for phototrophic sulfur oxidizers and diverse sulfur metabolisms. While Green Lake (Fayetteville, New York, NY) is known to host a diverse community of ecologically important sulfur bacteria, analyses of its microbial communities, to date, have been largely based on pigment analysis and smaller datasets from Sanger sequencing techniques. Here, we present the results of next‐generation sequencing of the eubacterial community in the context of the water column geochemistry. We observed abundant purple and green sulfur bacteria, as well as anoxygenic photosynthesis‐capable cyanobacteria within the upper monimolimnion. Amidst the phototrophs, we found other sulfur‐cycling bacteria including sulfur disproportionators and chemotrophic sulfur oxidizers, further detailing our understanding of the sulfur cycle and microbial ecology of euxinic, meromictic lakes.

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Lake Cadagno: Microbial Life in Crenogenic Meromixis

Cited by 23 publications

References 91 publications

Carbon isotope fractionation by anoxygenic phototrophic bacteria in euxinic Lake Cadagno

Carbon isotope fractionation by anoxygenic phototrophic bacteria in euxinic Lake Cadagno

Vertical niche occupation and potential metabolic interplay of microbial consortia in a deeply stratified meromictic model lake

Vertical structure of the bacterial diversity in meromictic Fayetteville Green Lake

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