Lambda replacement vectors carrying polylinker sequences

Frischauf, Anna‐Maria; Lehrach, Hans; Poustka, Annemarie; Murray, Noreen E.

doi:10.1016/s0022-2836(83)80190-9

Cited by 1,710 publications

(616 citation statements)

References 26 publications

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“…Fragments of 14-24 kb were enriched by centrifugation through a sucrose density gradient and used to construct a genomic library in EMBL3 h cloning vector as described [12].…”

Section: Methodsmentioning

confidence: 99%

The domain structure of the inhibitor subunit of human inter‐α‐trypsin inhibitor reflects the exon structure of its gene

Vetr¹,

Kögler²,

Gebhard³

1989

FEBS Letters

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The gene coding for the inhibitor subunit of the human plasma protein complex inter-u-trypsin inhibitor has been cloned. The exon structure of the gene corresponds with the organization of the protein in two distinct inhibitor domains. The exons coding for the inhibitor domains each comprise the complete information for a typical Kunitz-type proteinase inhibitor structure. In contrast to bovine aprotinin, the mature inhibitor protein contains amino acid residues flanking the inhibitor domains on both sides. These amino acid residues are encoded by additional exons.

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“…Fragments of 14-24 kb were enriched by centrifugation through a sucrose density gradient and used to construct a genomic library in EMBL3 h cloning vector as described [12].…”

Section: Methodsmentioning

confidence: 99%

The domain structure of the inhibitor subunit of human inter‐α‐trypsin inhibitor reflects the exon structure of its gene

Vetr¹,

Kögler²,

Gebhard³

1989

FEBS Letters

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“…Two genomie libraries from human kidney and brain tissue in lambda EMBL-4 phages were constructed by the method of Frischauf [6]. 2,3 x 106 phages were screened by hybridization with the eDNA of CSAP [5] which was labeled before with ~2p by random priming using a kit from Pharmacia (Freiburg, Germany).…”

Section: Isolation Of Genomic Clonesmentioning

confidence: 99%

The organization of the gene for the human cerebroside sulfate activator protein

et al. 1991

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The organization of 14 axons cov~rlnlft 97% or the eDNA sequence of human c~rebrosid¢, sulfate activator protein precursor has been determined from two overlapping EMBL,4 human genomic clones extending over 17 kb, All axons ~md exonlintron splic~ junctions and five introns were sequenced, Exon 8 consists of only 9 bp and is involved in rdternative spliclnlt which ~enerates three different mRNAs ofcercbrosidc sulfate activator precursor.Cerebroside sulfate activator protein; Genomic structure; Alternative splicinlI

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“…Escherichia coli strains DH5a [6] and XL1-Blue (Stratagene) were the host strains for the plasraids pUC9 [7] and pBluescript (Stratagene), respectively. E. coil strains NM 538 and NM 539 were host strains for propagation of phage A EMBL3 and its derivatives [8]. Bacterial cells were grown in LB media under the appropriate selective conditions [9].…”

Section: Strains and Culture Conditionsmentioning

confidence: 99%

“…Then about 80 ng Aspergillus DNA fragments were ligated to 500 ng vector arms. After in vitro packaging, recombinant phages were selected according to their ability to grow in cells of E. coli NM 539 carrying prophage P2 [8].…”

Section: Isolation Of Chromosomal Dna and Construction Of A Genomic Lmentioning

confidence: 99%

Cloning and DNA sequence analysis of the glucose oxidase gene from Aspergillus niger NRRL‐3

et al. 1989

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A cDNA library from Aspergillus niger strain NRRL‐3 enriched in sequences encoding the glucose oxidase was constructed. An 800 bp cDNA clone isolated from this library was used to screen 12 000 recombinant phages from an EMBL3 genomic library. A 15 kbp DNA segment isolated from this library contained the 1815 bp structural gene for glucose oxidase as well as a short 5′‐ and a longer 3′‐noncoding region. The deduced protein sequence was verified by partial peptide sequencing.

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Lambda replacement vectors carrying polylinker sequences

Cited by 1,710 publications

References 26 publications

The domain structure of the inhibitor subunit of human inter‐α‐trypsin inhibitor reflects the exon structure of its gene

The domain structure of the inhibitor subunit of human inter‐α‐trypsin inhibitor reflects the exon structure of its gene

The organization of the gene for the human cerebroside sulfate activator protein

Cloning and DNA sequence analysis of the glucose oxidase gene from Aspergillus niger NRRL‐3

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