Laminar organization of the early developing anterior hypothalamus

Caqueret, Aurore; Boucher, Francine; Michaud, Jacques L.

doi:10.1016/j.ydbio.2006.06.019

Cited by 55 publications

(65 citation statements)

References 29 publications

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“…Another possible explanation for this selective compensatory activity of Ldb2 may relate to its levels of expression (Caqueret et al, 2006), which may not be sufficient to fully compensate for Ldb1 loss at all developmental stages and in all cell types. Our experiments indicate that, in the RPCs, Ldb2 is sufficient to mediate Lhx2 activity, whereas studies in the limb bud show that Ldb2 is necessary for Isl1 activity (Tzchori et al, 2009;Narkis et al, 2012).…”

Section: The Unequal Role Of Ldb1 and Ldb2 In Retinogenesismentioning

confidence: 99%

The stage-dependent roles of Ldb1 and functional redundancy with Ldb2 in mammalian retinogenesis

et al. 2016

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The Lim domain-binding proteins are key co-factor proteins that assemble with LIM domains of the LMO/LIM-HD family to form functional complexes that regulate cell proliferation and differentiation. Using conditional mutagenesis and comparative phenotypic analysis, we analyze the function of Ldb1 and Ldb2 in mouse retinal development, and demonstrate overlapping and specific functions of both proteins. Ldb1 interacts with Lhx2 in the embryonic retina and both Ldb1 and Ldb2 play a key role in maintaining the pool of retinal progenitor cells. This is accomplished by controlling the expression of the Vsx2 and Rax, and components of the Notch and Hedgehog signaling pathways. Furthermore, the Ldb1/Ldb2-mediated complex is essential for generation of earlyborn photoreceptors through the regulation of Rax and Crx. Finally, we demonstrate functional redundancy between Ldb1 and Ldb2. Ldb1 can fully compensate the loss of Ldb2 during all phases of retinal development, whereas Ldb2 alone is sufficient to sustain activity of Lhx2 in both early-and late-stage RPCs and in Müller glia. By contrast, loss of Ldb1 disrupts activity of the LIM domain factors in neuronal precursors. An intricate regulatory network exists that is mediated by Ldb1 and Ldb2, and promotes RPC proliferation and multipotency; it also controls specification of mammalian retina cells.

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Section: The Unequal Role Of Ldb1 and Ldb2 In Retinogenesismentioning

confidence: 99%

The stage-dependent roles of Ldb1 and functional redundancy with Ldb2 in mammalian retinogenesis

et al. 2016

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“…Hypothalamic neurogenesis occurs between embryonic day 10 (E10) and E16, with individual hypothalamic nuclei typically being generated in an outsideinside sequence (Ifft, 1972;Altman and Bayer, 1986). Apparently, a lateromedial gradient exists along the whole hypothalamus, and possibly a dorsoventral gradient (Ifft, 1972;Wyss and Sripanidkulchai, 1985;Caqueret et al, 2005Caqueret et al, , 2006McClellan et al, 2006McClellan et al, , 2008, and this neurogenetic activity is still observed in the adult (Kokoeva et al, 2005). In the chick, two phases in the neurogenesis of the hypothalamus have been defined.…”

Section: Hypothalamic Subdivisions: Comparative Aspectsmentioning

confidence: 99%

“…The transcription factors Sim1, Arnt2, and Otp are coexpressed in this anterior hypothalamic portion and are essential for terminal differentiation of the neurosecretory neurons (Michaud et al, 1998(Michaud et al, , 2000Acampora et al;1999;Wang and Lufkin, 2000;Hosoya et al, 2001). One of their common downstream genes, Brn2, is necessary for the differentiation of the cells producing oxytocin, vasopressin, and CRH, whereas Sim2, a paralog of Sim1, contributes to the expression of TRH and somatostatin genes (Caqueret et al, 2006). Inactivation of any of these genes results in the elimination of the paraventricular and supraoptic structures and neuroendocrine hormone expression (reviewed in Markakis, 2002).…”

Section: Supraoptoparaventricular Areamentioning

confidence: 99%

Subdivisions of the turtle Pseudemys scripta hypothalamus based on the expression of regulatory genes and neuronal markers

Moreno

Domı́nguez

Morona

et al. 2011

J of Comparative Neurology

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The patterns of distribution of a set of conserved brain developmental regulatory transcription factors and neuronal markers were analyzed in the hypothalamus of the juvenile turtle, Pseudemys scripta. Combined immunohistochemical techniques were used for the identification of the main boundaries and subdivisions in the optic, paraventricular, tuberal, and mammillary hypothalamic regions. The combination of Tbr1 and Pax6 with Nkx2.1 allowed identification of the boundary between the telencephalic preoptic area, rich in Nkx2.1 expression, and the prethalamic eminence, rich in Tbr1 expression. In addition, at this level Nkx2.2 expression defined the boundary between the telencephalon and the hypothalamus. The dorsalmost hypothalamic domain was the supraoptoparaventricular region that was defined by the expression of Otp/Pax6 and the lack of Nkx2.1/Isl1. It is subdivided into rostral, rich in Otp and Nkx2.2, and caudal, only Otp-positive, portions. Ventrally, the suprachiasmatic area was identified by its catecholaminergic groups and the lack of Otp, and could be further divided into a rostral portion, rich in Nkx2.1 and Nkx2.2, and a caudal portion, rich in Isl1 and devoid of Nkx2.1 expression. The expressions of Nkx2.1 and Isl1 defined the tuberal hypothalamus, whereas only the rostral portion expressed Otp. Its caudal boundary was evident by the lack of Isl1 in the adjacent mammillary area, which expressed Nkx2.1 and Otp. All these results provide an important set of data on the interpretation of the hypothalamic organization in a reptile, and hence make a useful contribution to the understanding of hypothalamic evolution.

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“…In contrast to the restricted influence of SF-1 on VMH development, loss of nkx2.1 (Titf1) results in severe disruption of the entire hypothalamus except for the most ventral aspect as evidenced by an altered or absent expression pattern of SF-1, Sim-1, and Pax-6 (Marin et al, 2002). Other transcription factors on our list include ldb2, which has been linked to hypothalamic development (Caqueret et al, 2006) and Fezf1 (Hirata et al, 2006), COUP-TFII (Tripodi et al, 2004), and Satb2 (Britanova et al, 2005), which are all implicated broadly in neural development. Although the functional relevance of these other transcription factors in the VMH remains to be determined, it is likely that they will participate in specifying VMH patterning or cell fate.…”

Section: The Neonatal Mouse Vmh Transcriptomementioning

confidence: 99%

The Neonatal Ventromedial Hypothalamus Transcriptome Reveals Novel Markers with Spatially Distinct Patterning

Kurrasch¹,

Cheung²,

Lee³

et al. 2007

J. Neurosci.

155

151

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The ventromedial hypothalamus (VMH) is a distinct morphological nucleus involved in feeding, fear, thermoregulation, and sexual activity. It is essentially unknown how VMH circuits underlying these innate responses develop, in part because the VMH remains poorly defined at a cellular and molecular level. Specifically, there is a paucity of cell-type-specific genetic markers with which to identify neuronal subgroups and manipulate development and signaling in vivo. Using gene profiling, we now identify ϳ200 genes highly enriched in neonatal (postnatal day 0) mouse VMH tissue. Analyses of these VMH markers by real or virtual (Allen Brain Atlas; http:// www.brain-map.org) experiments revealed distinct regional patterning within the newly formed VMH. Top neonatal markers include transcriptional regulators such as Vgll2, SF-1, Sox14, Satb2, Fezf1, Dax1, Nkx2-2, and COUP-TFII, but interestingly, the highest expressed VMH transcript, the transcriptional coregulator Vgll2, is completely absent in older animals. Collective results from zebrafish knockdown experiments and from cellular studies suggest that a subset of these VMH markers will be important for hypothalamic development and will be downstream of SF-1, a critical factor for normal VMH differentiation. We show that at least one VMH marker, the AT-rich binding protein Satb2, was responsive to the loss of leptin signaling (Lep ob/ob ) at postnatal day 0 but not in the adult, suggesting that some VMH transcriptional programs might be influenced by fetal or early postnatal environments. Our study describing this comprehensive "VMH transcriptome" provides a novel molecular toolkit to probe further the genetic basis of innate neuroendocrine behavioral responses.

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Laminar organization of the early developing anterior hypothalamus

Cited by 55 publications

References 29 publications

The stage-dependent roles of Ldb1 and functional redundancy with Ldb2 in mammalian retinogenesis

The stage-dependent roles of Ldb1 and functional redundancy with Ldb2 in mammalian retinogenesis

Subdivisions of the turtle Pseudemys scripta hypothalamus based on the expression of regulatory genes and neuronal markers

The Neonatal Ventromedial Hypothalamus Transcriptome Reveals Novel Markers with Spatially Distinct Patterning

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