Laminin Functionalized Biomimetic Nanofibers for Nerve Tissue Engineering

Junka, Radoslaw; Valmikinathan, Chandra M.; Kalyon, Dilhan M.; Yu, Xiaojun

doi:10.1166/jbt.2013.1110

Cited by 49 publications

(28 citation statements)

References 48 publications

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“…In this respect, bioscaffolds yielded by cryogelation of gelatin or dextran linked to laminin [351] or PCL-chitosan scaffolds with surfaces modified via the crosslinking of laminin [352] could serve as versatile substrates with excellent mechanical and surface properties for in vivo cell delivery, resulting in highly neuroregenerative properties for nerve tissue engineering applications. Furthermore, the application of laminin-modified linear ordered collagen biomaterials loaded with laminin-binding ciliary neurotrophic factor (CNTF) was beneficial for sciatic nerve regeneration and functional recovery when tested in a rat sciatic-nerve transection model [353].…”

Section: Human Tissue Ecm-based Biomaterialsmentioning

confidence: 99%

Advancing biomaterials of human origin for tissue engineering

Chen

Liu

2016

Progress in Polymer Science

959

513

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Biomaterials have played an increasingly prominent role in the success of biomedical devices and in the development of tissue engineering, which seeks to unlock the regenerative potential innate to human tissues/organs in a state of deterioration and to restore or reestablish normal bodily function. Advances in our understanding of regenerative biomaterials and their roles in new tissue formation can potentially open a new frontier in the fast-growing field of regenerative medicine. Taking inspiration from the role and multi-component construction of native extracellular matrices (ECMs) for cell accommodation, the synthetic biomaterials produced today routinely incorporate biologically active components to define an artificial in vivo milieu with complex and dynamic interactions that foster and regulate stem cells, similar to the events occurring in a natural cellular microenvironment. The range and degree of biomaterial sophistication have also dramatically increased as more knowledge has accumulated through materials science, matrix biology and tissue engineering. However, achieving clinical translation and commercial success requires regenerative biomaterials to be not only efficacious and safe but also cost-effective and convenient for use and production. Utilizing biomaterials of human origin as building blocks for therapeutic purposes has provided a facilitated approach that closely mimics the critical aspects of natural tissue with regard to its physical and chemical properties for the orchestration of wound healing and tissue regeneration. In addition to directly using tissue transfers and transplants for repair, new applications of human-derived biomaterials are now focusing on the use of naturally occurring biomacromolecules, decellularized ECM scaffolds and autologous preparations rich in growth factors/non-expanded stem cells to either target acceleration/magnification of the body's own repair capacity or use nature's paradigms to create new tissues for restoration. In particular, there is increasing interest in separating ECMs into simplified functional domains and/or biopolymeric assemblies so that these components/constituents can be discretely exploited and manipulated for the production of bioscaffolds and new biomimetic biomaterials. Here, following an overview of tissue auto-/allo-transplantation, we discuss the recent trends and advances as well as the challenges and future directions in the evolution and application of human-derived biomaterials for reconstructive surgery and tissue engineering. In particular, we focus on an exploration of the structural, mechanical, biochemical and biological information present in native human tissue for bioengineering applications and to provide inspiration for the design of future biomaterials.

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Section: Human Tissue Ecm-based Biomaterialsmentioning

confidence: 99%

Advancing biomaterials of human origin for tissue engineering

Chen

Liu

2016

Progress in Polymer Science

959

513

View full text Add to dashboard Cite

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“…Table 1. [190], [175], [185], [186], [191], [192], [187], [193], [188], [189], [194] Although growth factors show potential for use in tissue regeneration and repair, they have various drawbacks. In general, protein instability, high manufacturing cost, and immunogenicity are the common problems associated with protein-based therapeutic strategies.…”

Section: Growth Factor Deliverymentioning

confidence: 99%

“…Peripheral nerve In vivo: rat tibial nerve defect repair [191] Laminin PCL-chitosan nanofibers Peripheral nerve In vitro: Schwann cells [192] NGF Silk fibroin/P(LLA-CL)-blended nanofiber Peripheral nerve [194] …”

Section: Musculoskeletal Tissuementioning

confidence: 99%

Electrospinning of polymer nanofibers for tissue regeneration

Jiang

Carbone

et al. 2015

Progress in Polymer Science

437

242

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Repair and regeneration of human tissues and organs using biomaterials, cells, and/or growth factors is a great challenge for tissue engineers and surgeons. The convergence of advanced materials science, nanotechnology, stem cell science, and developmental biology, which we define as Regenerative Engineering, represents the next multidisciplinary paradigm to engineer complex tissues. One of the grand challenges in this field is to mimic closely the hierarchical architecture and properties of the extracellular matrices (ECM) of the native tissues.A bio-inspired approach to creating biomaterials with nanoscale topographical features, microand macroscale gradient structures, and biological domains to interact with target growth factors and cells is key to overcoming this challenge for successful tissue regeneration. Furthermore, the healing and repair of diseased musculoskeletal tissues rely on many signaling pathways, involving numerous growth factors and their receptors. Thus, pharmacological manipulation of the signaling pathways with bioactive molecules is an important component of tissue regeneration. This review summarizes current strategies to develop advanced nanofibrous polymer-based scaffolds via electrospinning, their applications in regenerating human musculoskeletal tissues, and the use of polymer nanofibers to deliver growth factors or small molecules for regenerative medicine.

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“…3). Although laminin has been used in numerous experiments to enhance cell attachment and proliferation [31, 37–39], cells grown on scaffolds with covalently bound laminin through EDC/NHS mechanism did not show higher proliferation rates than cells grown on decellularized cell sheets. The experiment indicates the need for protein coating to enhance cell attachment and proliferation.…”

Section: Discussionmentioning

confidence: 99%

Novel Acellular Scaffold Made from Decellularized Schwann Cell Sheets for Peripheral Nerve Regeneration

Junka

2015

Regen. Eng. Transl. Med.

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Extracellular matrix surrounding Schwann cells and neurons provides critical determinants of cellular phenotype during development as well as essential cues in stimulating and guiding regrowth. Using cell sheet technology, we developed a novel scaffold enriched with native extracellular matrix from Schwann cells. Schwann cells were grown into sheets and layered onto polycaprolactone fibers for support. Upon decellularization of these constructs, extracellular matrix remained with few traces of nucleic acids. This method of deposition of extracellular matrix provided more protein than traditional seeding method after decellularization. Additionally, the isolated matrix supported proliferation of Schwann cells better than covalently bound laminin. The proliferation and differentiation of Schwann cells grown on decellularized sheets were complemented by upregulation of Erbb2 and myelin protein zero. Laminin expression of β1 and γ1 chains was also elevated. PC12 cells grown on decellularized sheets produced longer neurite extensions than aligned polycaprolactone fibers alone, proving potential of these scaffolds to be used in future peripheral nerve regenerative studies. Lay Summary Peripheral nerve injuries present a serious clinical need with approximately 50 % of surgical cases achieving only some restoration of function. In order to better guide regenerating nerves, supporting cells of the nerve tissue were grown into sheets and subsequently decellularized, leaving a myriad of surrounding protein as a scaffold. Constructs have been shown to support cell growth and neurite extension in vitro. Future projects will combine various cell types present in the nerve tissue as well as stem cells to fully support and reconstruct architecture of the peripheral nerves.

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Laminin Functionalized Biomimetic Nanofibers for Nerve Tissue Engineering

Cited by 49 publications

References 48 publications

Advancing biomaterials of human origin for tissue engineering

Advancing biomaterials of human origin for tissue engineering

Electrospinning of polymer nanofibers for tissue regeneration

Novel Acellular Scaffold Made from Decellularized Schwann Cell Sheets for Peripheral Nerve Regeneration

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