Laminin<i>α</i>2-secreting fibroblasts enhance the therapeutic effect of skeletal myoblast sheets

Uchinaka, Ayako; Tasaka, K; Mizuno, Yuji; Maeno, Yoshitaka; Ban, Tsuyoshi; Mori, Satoshi; Hamada, Y.; Miyagawa, Shigeru; Saito, Atsuhiro; Sawa, Yoshiki; Matsuura, Nariaki; Nagata, Kazuya; Yamamoto, Hirofumi; Kawaguchi, Naomasa

doi:10.1093/ejcts/ezw296

Cited by 8 publications

(9 citation statements)

References 18 publications

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“…After confirming the adherence of the first sheet, subsequent sheets were applied using the same technique, as described previously. [21][22][23]…”

Section: Western Blot Analysismentioning

confidence: 99%

“…The cardiac function of the treated rats was monitored by echocardiography both before surgery and at 2, 4, 6, and 8 weeks after gel implantation or at 2 and 4 weeks after cell sheet implantation using a system equipped with a 12-MHz transducer and a SONOS 5500 device (Agilent Technologies, Santa Clara, Calif) under isoflurane inhalation (2%), as described previously. 19,22,23…”

Section: Measurement Of Cardiac Function By Echocardiographymentioning

confidence: 99%

“…At 4 or 8 weeks after treatment, the excised and fixed LV myocardium specimens were processed for histology as described previously 22,23 (also see Supplemental Methods).…”

Section: Histological Analysismentioning

confidence: 99%

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Overexpression of collagen type III in injured myocardium prevents cardiac systolic dysfunction by changing the balance of collagen distribution

Uchinaka

Yoshida

Tanaka

et al. 2018

The Journal of Thoracic and Cardiovascular Surgery

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“…After confirming the adherence of the first sheet, subsequent sheets were applied using the same technique, as described previously. [21][22][23]…”

Section: Western Blot Analysismentioning

confidence: 99%

Section: Measurement Of Cardiac Function By Echocardiographymentioning

confidence: 99%

See 1 more Smart Citation

Overexpression of collagen type III in injured myocardium prevents cardiac systolic dysfunction by changing the balance of collagen distribution

Uchinaka

Yoshida

Tanaka

et al. 2018

The Journal of Thoracic and Cardiovascular Surgery

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“…Thus the ECM is the “theatre” where most cues or signals from diverse sources are integrated into a “specific” message that is relayed to cells. The ECM is synthesised mostly by fibroblasts with the contribution of other cells such as mesenchymal stem cells, macrophages and endothelial cells [ 31 , 32 , 33 , 34 , 35 , 36 ]. Fibroblasts are the main cellular components of tumor stroma [ 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 ].…”

Section: Introductionmentioning

confidence: 99%

The Role of Tumor Microenvironment in Chemoresistance: 3D Extracellular Matrices as Accomplices

Senthebane

Jonker

Rowe

et al. 2018

IJMS

113

176

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Background: The functional interplay between tumor cells and their adjacent stroma has been suggested to play crucial roles in the initiation and progression of tumors and the effectiveness of chemotherapy. The extracellular matrix (ECM), a complex network of extracellular proteins, provides both physical and chemicals cues necessary for cell proliferation, survival, and migration. Understanding how ECM composition and biomechanical properties affect cancer progression and response to chemotherapeutic drugs is vital to the development of targeted treatments. Methods: 3D cell-derived-ECMs and esophageal cancer cell lines were used as a model to investigate the effect of ECM proteins on esophageal cancer cell lines response to chemotherapeutics. Immunohistochemical and qRT-PCR evaluation of ECM proteins and integrin gene expression was done on clinical esophageal squamous cell carcinoma biopsies. Esophageal cancer cell lines (WHCO1, WHCO5, WHCO6, KYSE180, KYSE 450 and KYSE 520) were cultured on decellularised ECMs (fibroblasts-derived ECM; cancer cell-derived ECM; combinatorial-ECM) and treated with 0.1% Dimethyl sulfoxide (DMSO), 4.2 µM cisplatin, 3.5 µM 5-fluorouracil and 2.5 µM epirubicin for 24 h. Cell proliferation, cell cycle progression, colony formation, apoptosis, migration and activation of signaling pathways were used as our study endpoints. Results: The expression of collagens, fibronectin and laminins was significantly increased in esophageal squamous cell carcinomas (ESCC) tumor samples compared to the corresponding normal tissue. Decellularised ECMs abrogated the effect of drugs on cancer cell cycling, proliferation and reduced drug induced apoptosis by 20–60% that of those plated on plastic. The mitogen-activated protein kinase-extracellular signal-regulated kinase (MEK-ERK) and phosphoinositide 3-kinase-protein kinase B (PI3K/Akt) signaling pathways were upregulated in the presence of the ECMs. Furthermore, our data show that concomitant addition of chemotherapeutic drugs and the use of collagen- and fibronectin-deficient ECMs through siRNA inhibition synergistically increased cancer cell sensitivity to drugs by 30–50%, and reduced colony formation and cancer cell migration. Conclusion: Our study shows that ECM proteins play a key role in the response of cancer cells to chemotherapy and suggest that targeting ECM proteins can be an effective therapeutic strategy against chemoresistant tumors.

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“…Therefore, in future research, we should focus more on the combination of multiple cell sources in the fabrication of cell sheet. For example, some papers showed that the combination of fibroblasts expressing laminin G-module 4-5 of α2 and SMs in multilayered sheet markedly inhibited the detachment of implanted myoblasts from sheet, resulting in more permanent and stable efficacy on single myoblast sheet because fibroblasts could abundantly embed in the myoblast layer to fasten and support the structure via extracellular matrix production [58]. Another report showed that the local release of VEGF enhanced the transplantation efficiency of layered cardiomyocyte sheet, which could be related to vascularization [70].…”

Section: Mesenchymal Stem Cell-derived Cell Sheetmentioning

confidence: 99%

Stem cell-derived cell sheet transplantation for heart tissue repair in myocardial infarction

et al. 2020

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Stem cell-derived sheet engineering has been developed as the next-generation treatment for myocardial infarction (MI) and offers attractive advantages in comparison with direct stem cell transplantation and scaffold tissue engineering. Furthermore, induced pluripotent stem cell-derived cell sheets have been indicated to possess higher potential for MI therapy than other stem cell-derived sheets because of their capacity to form vascularized networks for fabricating thickened human cardiac tissue and their long-term therapeutic effects after transplantation in MI. To date, stem cell sheet transplantation has exhibited a dramatic role in attenuating cardiac dysfunction and improving clinical manifestations of heart failure in MI. In this review, we retrospectively summarized the current applications and strategy of stem cell-derived cell sheet technology for heart tissue repair in MI.

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Lamininα2-secreting fibroblasts enhance the therapeutic effect of skeletal myoblast sheets

Abstract: Laminin secreted by implanted fibroblasts inhibited the detachment of implanted myoblasts from grafted myocardia, resulting in more permanent therapeutic effects upon myoblast sheet transplantation.

Cited by 8 publications

References 18 publications

Overexpression of collagen type III in injured myocardium prevents cardiac systolic dysfunction by changing the balance of collagen distribution

Overexpression of collagen type III in injured myocardium prevents cardiac systolic dysfunction by changing the balance of collagen distribution

The Role of Tumor Microenvironment in Chemoresistance: 3D Extracellular Matrices as Accomplices

Stem cell-derived cell sheet transplantation for heart tissue repair in myocardial infarction

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