Laminins in osteogenic differentiation and pluripotency maintenance

Chao-zhen, Chen; Jiang, Zhiwei; Yang, Guoli

doi:10.1016/j.diff.2020.05.002

Cited by 17 publications

(10 citation statements)

References 46 publications

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“…N-cadherin is a key factor in directing cell–cell interaction during the mesenchymal condensation and it is also considered as a mimetic peptide that enhanced the osteogenic differentiation of the MSCs, leading to bone matrix deposition on the titanium implants [ 51 ]. Laminins are glycoproteins and major structural components in the basal lamina and showed a critical role to cell adhesion, differentiation and migration [ 52 ].…”

Section: Discussionmentioning

confidence: 99%

Human Periodontal Ligament Stem Cells Response to Titanium Implant Surface: Extracellular Matrix Deposition

Marconi

Fonticoli

Rocca

et al. 2021

Biology

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The major challenge for dentistry is to provide the patient an oral rehabilitation to maintain healthy bone conditions in order to reduce the time for loading protocols. Advancement in implant surface design is necessary to favour and promote the osseointegration process. The surface features of titanium dental implant can promote a relevant influence on the morphology and differentiation ability of mesenchymal stem cells, induction of the osteoblastic genes expression and the release of extracellular matrix (ECM) components. The present study aimed at evaluating the in vitro effects of two different dental implants with titanium surfaces, TEST and CTRL, to culture the human periodontal ligament stem cells (hPDLSCs). Expression of ECM components such as Vimentin, Fibronectin, N-cadherin, Laminin, Focal Adhesion Kinase (FAK) and Integrin beta-1 (ITGB1), and the osteogenic related markers, as runt related transcription factor 2 (RUNX2) and alkaline phosphatase (ALP), were investigated. Human PDLSCs cultured on the TEST implant surface demonstrated a better cell adhesion capability as observed by Scanning Electron Microscopy (SEM) and immunofluorescence analysis. Moreover, immunofluorescence and Western blot experiments showed an over expression of Fibronectin, Laminin, N-cadherin and RUNX2 in hPDLSCs seeded on TEST implant surface. The gene expression study by RT-PCR validated the results obtained in protein assays and exhibited the expression of RUNX2, ALP, Vimentin (VIM), Fibronectin (FN1), N-cadherin (CDH2), Laminin (LAMB1), FAK and ITGB1 in hPDLSCs seeded on TEST surface compared to the CTRL dental implant surface. Understanding the mechanisms of ECM components release and its regulation are essential for developing novel strategies in tissue engineering and regenerative medicine. Our results demonstrated that the impact of treated surfaces of titanium dental implants might increase and accelerate the ECM apposition and provide the starting point to initiate the osseointegration process.

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Section: Discussionmentioning

confidence: 99%

Human Periodontal Ligament Stem Cells Response to Titanium Implant Surface: Extracellular Matrix Deposition

Marconi

Fonticoli

Rocca

et al. 2021

Biology

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“…The FAK/PI3K/AKT signaling pathways could play a crucial role in promoting osteogenic differentiation. [34][35][36] For example, Liu et al reported that platelet-rich plasma (PRP) could improve the healing effect of mandibular alveolar bone defects by activating the FAK/PI3K/ AKT signaling pathways. 37 Schreiber et al reported that disorders of FAK/PI3K/AKT signaling would inhibit the differentiation of MSCs to osteoblasts.…”

Section: Discussionmentioning

confidence: 99%

A regulatory mechanism of a stepwise osteogenesis-mimicking decellularized extracellular matrix on the osteogenic differentiation of bone marrow-derived mesenchymal stem cells

Zheng

Yao

et al. 2022

J. Mater. Chem. B

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“…In addition, integrin-ECM interaction was identified to be capable of triggering the signaling mechanism of osteoblast specific gene expression. For instance, integrin α5 in the ECM bound to intracellular protein kinases and phosphatases such as focal adhesion kinase (FAK) and PI3K, and participated in signal transduction through PI3K-Akt pathway, which could activate the phosphorylation levels of Runx2 and promoted osteogenic gene levels. , Moreover, the ECM can also activate the FAK-ERK signaling pathway through integrin α2, upregulate the expression of Runx2 and ALP, and induce osteoblastic differentiation. ,, By comparing the osteogenic differentiation ability of MSCs on ECM with different stiffness, the important regulation of the classical Wnt/β-catenin pathway in ECM-induced osteogenic differentiation is noted …”

Section: Discussionmentioning

confidence: 99%

“…41,42 Moreover, the ECM can also activate the FAK-ERK signaling pathway through integrin α2, upregulate the expression of Runx2 and ALP, and induce osteoblastic differentiation. 41,43,44 By comparing the osteogenic differentiation ability of MSCs on ECM with different stiffness, the important regulation of the classical Wnt/β-catenin pathway in ECM-induced osteogenic differentiation is noted. 41 Overall, it would be confidently stated that ECM/PLGA scaffolds contained a variety of active ECM components and could upregulate the expressions of Runx2, BSP, OPN, OCN, and endogenous growth factors by activating one or more signal pathways such as the MAPK pathway, integrin-dependent pathway, Wnt/catenin pathway, and BMP/Smad pathway, thereby enhancing the osteogenic differentiation of MSCs (the possible regulatory mechanism of ECM on osteoblastogenesis is shown in Figure 11).…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Coordination of Osteoblastogenesis and Osteoclastogenesis by the Bone Marrow Mesenchymal Stem Cell-Derived Extracellular Matrix To Promote Bone Regeneration

Yin

et al. 2022

ACS Appl. Bio Mater.

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Extracellular matrix (ECM)-based therapies have been developed to improve bone repair because of their abundance of bioactive components. Besides the osteogenic promotion and the immune response, the potential effect of the ECM on the coordination between osteoblastogenesis and osteoclastogenesis in vivo should also deserve great attention because both osteoblasts and osteoclasts get involved in bone regeneration and are critical for the final repair outcome. Herein, based on our previous study on decellularization, antigen removal, and growth factor retention, porous poly (lactic-co-glycolic acid) (PLGA) scaffolds decorated with the bone marrow mesenchymal stem cell (BMSC)-derived ECM were prepared, and the functions of the ECM on BMSC osteogenic differentiation and osteoclastogenesis in vitro were preferentially investigated. Afterward, bone regeneration and osteoclast formation in vivo induced by ECM-decorated PLGA scaffolds were further studied. The in vitro tests revealed that ECM-decorated PLGA scaffolds obviously facilitated BMSC proliferation and osteogenic differentiation. However, when osteoclast precursors were cultured on the BMSC-derived ECM, the number and size of osteoclasts, expression of cathepsin K and matrix metalloproteinase 9, and tartrate-resistant acid phosphatase activity were notably decreased, accompanied by the reduction in the reactive oxygen species (ROS) level. Interestingly, the addition of exogenous hydrogen peroxide elevated the osteoclast amount on the ECM and up-regulated the resorption-related enzyme levels, implying that the repressive effect of the BMSC-derived ECM on osteoclasts may be related to the intracellular ROS. After implantation into calvarial defects, the ECM-decorated PLGA scaffolds significantly increased bone volume and bone mineral density compared with bare PLGA scaffolds and did not stimulate the overmuch formation of osteoclasts in vivo. This study evidenced that the BMSC-derived ECM may coordinate osteoblastogenesis and osteoclastogenesis and promote favorable bone formation without stimulating bone resorption.

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Laminins in osteogenic differentiation and pluripotency maintenance

Cited by 17 publications

References 46 publications

Human Periodontal Ligament Stem Cells Response to Titanium Implant Surface: Extracellular Matrix Deposition

Human Periodontal Ligament Stem Cells Response to Titanium Implant Surface: Extracellular Matrix Deposition

A regulatory mechanism of a stepwise osteogenesis-mimicking decellularized extracellular matrix on the osteogenic differentiation of bone marrow-derived mesenchymal stem cells

Coordination of Osteoblastogenesis and Osteoclastogenesis by the Bone Marrow Mesenchymal Stem Cell-Derived Extracellular Matrix To Promote Bone Regeneration

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