LAMP-1-chimeric DNA vaccines enhance the antibody response in Japanese flounder, Paralichthys olivaceus

Characterization of three lamp genes from largemouth bass (Micropterus salmoides): molecular cloning, expression patterns, and their transcriptional levels in response to fast and refeeding strategy

“…The tissue expression patterns of the three genes were analyzed by qRT-PCR. The results showed that the lamp1 gene was widely distributed in all tissues examined, and the highly transcript level of lamp1 was found in the brain (Figure 7A), which is in line with previous studies in Japanese flounder (Rondón- Barragán et al, 2017), indicating that the lamp1 gene may be involved in neural activity. For instance, Barrachina et al (2006) revealed that the transcript level of lamp1 was increased in neurons and in glial cells surrounding senile plaques in patients with Alzheimer's diseases.…”

“…In the current study, three of the lamp family genes (lamp1, lamp2 and lamp3) of largemouth bass (M. salmoides) were successfully identified and characterized. The full-length coding sequences of lamp1, lamp2 and lamp3 were 1,251, 1,221 and 771bp, and predicted to encode a protein of 416, 406 and 256 amino acids, respectively, which is consistent with previous findings, such as in japanese flounder (Bandyopadhyay et al, 2014;Rondón-Barragán et al, 2017), zebrafish (GenBank no. NM_ 001013533) and rainbow trout (Johansson et al, 2012), indicating that the family of lamp1-3 genes may be conserved in fish.…”

“…Furthermore, all MsLAMP family proteins have a conserved tyrosine-based motif (TyrX-X-hydrophobic residue or YXXØ sorting signal) (412-416 aa for LAMP1 (Figure 2A), 402-406 aa for LAMP2 (Figure 2B) and 252-256 aa for LAMP3 (Figure 2C)) in the corresponding of short C-terminal cytoplasmic domain, which is required for lysosomal targeting (Guarnieri et al, 1993;Wu et al, 2010). Sequence alignment analysis further revealed that both of MsLAMP1 and MsLAMP2 possess eight conserved cysteine residues, but the MsLAMP3 has only four conserved cysteine residues with the potential to form four and two disulfide bridges, respectively (Rondón-Barragán et al, 2017). In addition, similar to other LAMP family members, a proline/serine-rich or proline/ threonine region was also found between the luminal and proximal domains in LAMP1-3 protein sequences.…”

“…Lysosomes, which may originate from pre-existing endolysosomes or autolysosomes (Mahapatra et al, 2021), are spherical bodies with amorphous of 50-500 nm diameter, single membrane, terminal degradation vesicular structures organelles, and are found in almost all eukaryotic cells (Debjyoti et al, 2014;Rondón-Barragán et al, 2017). Lysosomes are responsible for the degradation of cellular macromolecules (such as carbohydrates, proteins, lipids, and nucleic acids) into their constituent building blocks, which are generally obtained via phagocytosis or endocytosis, or autophagy (Alessandrini et al, 2017;Baba et al, 2020).…”

“…In fish, however, the lamp genes have received far too little attention in the literature. Only four orthologs of mammalian lamp genes, including lamp1, lamp2, lamp3 and lamp4 have been characterized in zebrafish (Danio rerio) (Meireles et al, 2018;Iyer et al, 2022), lamp1 was identified in japanese flounder (Paralichthys olivaceus) (Rondón- Barragán et al, 2017), lamp3 was isolated from rainbow trout (Oncorhynchus mykiss) (Johansson et al, 2012) and lamp4 was cloned from bluntnose black bream (Megalobrama amblycephala) (Cui et al, 2022). And the above results showed that lamp family genes play a vital immune role in teleosts.…”