LAMP-on-a-chip: Revising microfluidic platforms for loop-mediated DNA amplification

Zhang, Haoqing; Xu, Ying; Fohlerová, Zdenka; Chang, Honglong; Iliescu, Ciprian; Neužil, Pavel

doi:10.1016/j.trac.2019.01.015

Cited by 186 publications

(134 citation statements)

References 91 publications

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“…SENSR can be implemented in any diagnostic laboratory with commonly available instruments such as a water bath and a spectrophotometer. In addition, the full potential of SENSR would be realized through the development of field-deployable devices for isothermal incubation and fluorescence measurement 37,[40][41][42][43] . Due to the simplicity of the operating mechanism, SENSR can be further developed into a portable, easy-to-use test of paper-based or lateral-flow type, as exemplified by recent developments in nucleic acid diagnostics 9,[44][45][46][47] .…”

Section: Discussionmentioning

confidence: 99%

Sensitive fluorescence detection of SARS-CoV-2 RNA in clinical samples via one-pot isothermal ligation and transcription

et al. 2020

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The control of viral outbreaks requires nucleic acid diagnostic tests that are sensitive, simple and fast. Here, we report a highly sensitive and specific one-pot assay for the fluorescence-based detection of RNA from pathogens. The assay, which can be performed within 30-50 min of incubation time and can reach a limit of detection of 0.1-attomolar RNA concentration, relies on a sustained isothermal reaction cascade producing an RNA aptamer that binds to a fluorogenic dye. The RNA aptamer is transcribed by the T7 RNA polymerase from the ligation product of a promoter DNA probe and a reporter DNA probe that hybridize with the target single-stranded RNA sequence via the SplintR ligase (a Chlorella virus DNA ligase). In 40 nasopharyngeal SARS-CoV-2 samples, the assay reached positive and negative predictive values of 95 and 100%, respectively. We also show that the assay can rapidly detect a range of viral and bacterial RNAs.

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Section: Discussionmentioning

confidence: 99%

Sensitive fluorescence detection of SARS-CoV-2 RNA in clinical samples via one-pot isothermal ligation and transcription

et al. 2020

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“…Isothermal NA amplification methods (Zanoli and Spoto, 2013;Zhang et al, 2019) are carried out at a constant temperature, typically between �37 � C and �65 � C, making the isothermal amplification system hardware simpler than the one used for PCR. Many isothermal amplification methods have been reported and can be grouped based on the reaction principle.…”

Section: Isothermal Amplificationmentioning

confidence: 99%

Recent advances in lab-on-a-chip technologies for viral diagnosis

Zhu

Fohlerová

Pekárek

et al. 2020

Biosensors and Bioelectronics

195

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A B S T R A C TThe global risk of viral disease outbreaks emphasizes the need for rapid, accurate, and sensitive detection techniques to speed up diagnostics allowing early intervention. An emerging field of microfluidics also known as the lab-on-a-chip (LOC) or micro total analysis system includes a wide range of diagnostic devices. This review briefly covers both conventional and microfluidics-based techniques for rapid viral detection. We first describe conventional detection methods such as cell culturing, immunofluorescence or enzyme-linked immunosorbent assay (ELISA), or reverse transcription polymerase chain reaction (RT-PCR). These methods often have limited speed, sensitivity, or specificity and are performed with typically bulky equipment. Here, we discuss some of the LOC technologies that can overcome these demerits, highlighting the latest advances in LOC devices for viral disease diagnosis. We also discuss the fabrication of LOC systems to produce devices for performing either individual steps or virus detection in samples with the sample to answer method. The complete system consists of sample preparation, and ELISA and RT-PCR for viral-antibody and nucleic acid detection, respectively. Finally, we formulate our opinions on these areas for the future development of LOC systems for viral diagnostics.

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“…Loop-mediated isothermal amplification (LAMP) is another promising technique to quickly preconcentrate nucleic acids developed by Notomi et al in 2000 [156]. The advantage of LAMP over traditional PCR methods is the completion at a single set temperature (approximately 65 • C) within a single tube [157,158]. It involves a three-step process starting with sample material priming, amplification, and then elongation and further exponential amplification.…”

Section: How To Detect On-sitementioning

confidence: 99%

Integrated Electrochemical Biosensors for Detection of Waterborne Pathogens in Low-Resource Settings

et al. 2020

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More than 783 million people worldwide are currently without access to clean and safe water. Approximately 1 in 5 cases of mortality due to waterborne diseases involve children, and over 1.5 million cases of waterborne disease occur every year. In the developing world, this makes waterborne diseases the second highest cause of mortality. Such cases of waterborne disease are thought to be caused by poor sanitation, water infrastructure, public knowledge, and lack of suitable water monitoring systems. Conventional laboratory-based techniques are inadequate for effective on-site water quality monitoring purposes. This is due to their need for excessive equipment, operational complexity, lack of affordability, and long sample collection to data analysis times. In this review, we discuss the conventional techniques used in modern-day water quality testing. We discuss the future challenges of water quality testing in the developing world and how conventional techniques fall short of these challenges. Finally, we discuss the development of electrochemical biosensors and current research on the integration of these devices with microfluidic components to develop truly integrated, portable, simple to use and cost-effective devices for use by local environmental agencies, NGOs, and local communities in low-resource settings.

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LAMP-on-a-chip: Revising microfluidic platforms for loop-mediated DNA amplification

Cited by 186 publications

References 91 publications

Sensitive fluorescence detection of SARS-CoV-2 RNA in clinical samples via one-pot isothermal ligation and transcription

Sensitive fluorescence detection of SARS-CoV-2 RNA in clinical samples via one-pot isothermal ligation and transcription

Recent advances in lab-on-a-chip technologies for viral diagnosis

Integrated Electrochemical Biosensors for Detection of Waterborne Pathogens in Low-Resource Settings

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