LampPort: a handheld digital microfluidic device for loop-mediated isothermal amplification (LAMP)

Wan, Liang; Gao, Jie; Chen, Tianlan; Dong, Cheng; Li, Haoran; Wen, Yan-Zi; Lun, Zhao‐Rong; Jia, Yanwei; Mak, Pui-In; Martins, Rui P.

doi:10.1007/s10544-018-0354-9

Cited by 52 publications

(41 citation statements)

References 32 publications

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“…Some salient examples thereof are circulating tumor cells (CTC) of various types of cancer [1,[36][37][38], rare cells (e.g., sickle-cell variants of red blood cells) [39,40], parasites, like Plasmodium falciparum [1,7,10,11,[13][14][15][21][22][23]36,[41][42][43][44][45][46][47][48][49][50] and Trypanosoma spp. [8,44,[51][52][53][54][55][56][57] and even plant pathogens [26,58], as well as-after cells have been lysed-subcellular infection markers (e.g., DNA, RNA fragments) [10,11,22,43,[59][60][61][62]. Given the vast adaptability of microfluidics to any kind of single or multi-cellular assay [63], the ability to combine it with various light microscopy techniques…”

Section: What We Can Detectmentioning

confidence: 99%

“…An alternative technique for diagnosing sub-cellular targets is loop-mediated isothermal amplification (LAMP), which can run at a single temperature of around 65 • C and inside a single tube where the cell lysate is mixed with DNA polymerase and a set of four (or more) specifically designed primers [214]. The fact that it needs less equipment to be run, and that it can even be more portable [55], or fully non-instrumented [48], makes LAMP and its derivatives very promising for use in the field for resource-scarce settings [11,22,47,48,55,61].…”

Section: Polymerase Chain Reaction (Pcr Nested Pcr Qpcr/ Rt-pcr)mentioning

confidence: 99%

“…Even more advanced and exciting are LAMP-derived techniques, specifically designed for the deployment in areas with very limited access to resources, e.g., field work in endemic areas of parasitic diseases like Malaria, LAMPport (portable) [55] and non-instrumented nucleic acid amplification LAMP (NINA-LAMP) [48]. With further tweaking, it is possible to attain field-applicable multi-parasite or multi-disease tests at low costs-if it can be combined with a proper separation technique, like DLD or paper microfluidics.…”

Section: Polymerase Chain Reaction (Pcr Nested Pcr Qpcr/ Rt-pcr)mentioning

confidence: 99%

“…Point-of-Care (POC) means that these diagnostic devices can be used at the patient level and deliver a diagnosis locally and quickly-without need for taking samples, sending them away for analysis and having to continue care while waiting on the outcome of the diagnostic test. This is often achieved with the LAMP test and its derivatives, LAMPport and NINA-LAMP [22,47,48,55,61]. However, many other, especially microfluidic, approaches have also been tested and discussed for their aptitude to be used for POC diagnostic tools [38,45,205,212,[221][222][223][224][225].…”

Section: Point-of-care Diagnostics (Poc)mentioning

confidence: 99%

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Lab-on-a-Chip Technologies for the Single Cell Level: Separation, Analysis, and Diagnostics

Hochstetter

2020

Micromachines

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In the last three decades, microfluidics and its applications have been on an exponential rise, including approaches to isolate rare cells and diagnose diseases on the single-cell level. The techniques mentioned herein have already had significant impacts in our lives, from in-the-field diagnosis of disease and parasitic infections, through home fertility tests, to uncovering the interactions between SARS-CoV-2 and their host cells. This review gives an overview of the field in general and the most notable developments of the last five years, in three parts: 1. What can we detect? 2. Which detection technologies are used in which setting? 3. How do these techniques work? Finally, this review discusses potentials, shortfalls, and an outlook on future developments, especially in respect to the funding landscape and the field-application of these chips.

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Section: What We Can Detectmentioning

confidence: 99%

Section: Polymerase Chain Reaction (Pcr Nested Pcr Qpcr/ Rt-pcr)mentioning

confidence: 99%

Section: Polymerase Chain Reaction (Pcr Nested Pcr Qpcr/ Rt-pcr)mentioning

confidence: 99%

Section: Point-of-care Diagnostics (Poc)mentioning

confidence: 99%

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Lab-on-a-Chip Technologies for the Single Cell Level: Separation, Analysis, and Diagnostics

Hochstetter

2020

Micromachines

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“…Because the HV507 is not compatible with AC signals, a complicated solution is presented to address the electrodes individually [46]. A similar approach was published by Wan et al [47]. Unfortunately, they did not present detailed information about the used hardand software.…”

Section: Plos Onementioning

confidence: 99%

PortaDrop: A portable digital microfluidic platform providing versatile opportunities for Lab-On-A-Chip applications

et al. 2020

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Electrowetting-on-dielectric is a decent technique to manipulate discrete volumes of liquid in form of droplets. In the last decade, electrowetting-on-dielectric systems, also called digital microfluidic systems, became more frequently used for a variety of applications because of their high flexibility and reconfigurability. Thus, one design can be adapted to different assays by only reprogramming. However, this flexibility can only be useful if the entire system is portable and easy to use. This paper presents the development of a portable, standalone digital microfluidic system based on a Linux-based operating system running on a Raspberry Pi, which is unique. We present "PortaDrop" exhibiting the following key features: (1) an "all-in-one box" approach, (2) a user-friendly, self-explaining graphical user interface and easy handling, (3) the ability of integrated electrochemical measurements, (4) the ease to implement additional lab equipment via Universal Serial Bus and the General Purpose Interface Bus as well as (5) a standardized experiment documentation. We propose that PortaDrop can be used to carry out experiments in different applications, where small sample volumes in the nanoliter to picoliter range need to be handled an analyzed automatically. As a first application, we present a protocol, where a droplet is consequently exchanged by droplets of another medium using passive dispensing. The exchange is monitored by electrical impedance spectroscopy. It is the first time, the media exchange caused by passive dispensing is characterized by electrochemical impedance spectroscopy. Summarizing, PortaDrop allows easy combination of fluid handling by means of electrowetting and additional sensing.

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A low‐cost, portable, and practical LAMP device for point‐of‐diagnosis in the field

Kaymaz

Ergenç

Aytekin

et al. 2022

Biotech & Bioengineering

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Transition of rapid, ready‐to‐use, and low‐cost nucleic acid‐based detection technologies from laboratories to points of sample collection has drastically accelerated. However, most of these approaches are still incapable of diagnosis starting from sampling through nucleic acid isolation and detection in the field. Here we developed a simple, portable, low‐cost, colorimetric, and remotely controllable platform for reliable, high‐throughput, and rapid diagnosis using loop‐mediated isothermal amplification (LAMP) assays. It consists of a thermally isolated cup, low‐cost electronic components, a polydimethylsiloxane sample well, and a fast prototyped case that covers electronic components. The steady‐state temperature error of the system is <1%. We performed LAMP, Colony‐LAMP, and Colony polymerase chain reactions (PCRs) using the yaiO2 primer set for Escherichia coli and Pseudomonas aeruginosa samples at 65°C and 30 min. We detected the end‐point colorimetric readouts by the naked eye under day light. We confirmed the specificity and sensitivity of our approach using pure genomic DNA and crude bacterial colonies. We benchmarked our Colony‐LAMP detection against Colony PCR. The number of samples tested can easily be modified for higher throughput in our system. We strongly believe that our platform can greatly contribute rapid and reliable diagnosis in versatile operational environments.

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LampPort: a handheld digital microfluidic device for loop-mediated isothermal amplification (LAMP)

Cited by 52 publications

References 32 publications

Lab-on-a-Chip Technologies for the Single Cell Level: Separation, Analysis, and Diagnostics

Lab-on-a-Chip Technologies for the Single Cell Level: Separation, Analysis, and Diagnostics

PortaDrop: A portable digital microfluidic platform providing versatile opportunities for Lab-On-A-Chip applications

A low‐cost, portable, and practical LAMP device for point‐of‐diagnosis in the field

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