Palaeoenvironmental research in the Southern Levant presents a series of challenges, partly due to the unequal distribution of palaeoenvironmental records and potential archives throughout the region. Our knowledge of climatic evolution, during the last approximately 25 000 years, is of crucial importance to understand cultural developments. More local, well-dated, multi-proxy studies are much needed to obtain an accurate picture of environmental change in respect of the Late Pleistocene and the Holocene. This contribution reviews the current state of knowledge regarding Late Quaternary palaeoenvironmental changes in the Southern Levant, including some examples of more recent developments in palaeoenvironmental reconstruction in Israel and the Dead Sea area, and introduces the major challenges researchers face in the region. It also presents the first results of a new case study in Jordan, based on an analysis of peaty deposits located in the mountain slopes east of the Dead Sea. Such new studies help refine our knowledge of local environmental changes in the Southern Levant and especially the more arid areas, for which little information is presently available. More material suitable for palaeoenvironmental research, for example extensive tufa and travertine series, still awaits consideration in Jordan, opening up exciting perspectives for future research in the area.