Landscape and Dynamics of Single Immune Cells in Hepatocellular Carcinoma

A Single‐Cell Perspective of the Mammalian Liver in Health and Disease

“…More recently, Zhang et al used a combination of full-length and 3′ scRNA-seq technologies to generate a high-resolution map of the immune landscape of HCC. (28) By surveying multiple immune-relevant sites (tumor, adjacent liver, hepatic lymph node, blood, and ascites), the authors identified LAMP3 + dendritic cells as a key cell type that originated from tumor, migrated to hepatic lymph nodes, and shaped lymphocyte function through ligand-receptor signaling. These studies provided unprecedented insights into cellular heterogeneity, molecular nature, and functional implications of the immune landscape associated with human HCC.…”

Heterogeneity and targeted therapy-induced adaptations in lung cancer revealed by longitudinal single-cell RNA sequencing

“…Comparison of single-cell transcriptional profiles of individual cells at different treatment timepoints could lead to deeper insight into the evolution of cell states of both cancer cells and TME cells occurring during treatment. There is currently a paucity of single-cell transcriptome studies that sample metastatic solid malignancies and prior scR-NAseq studies of metastatic disease largely focused on individual treatment timepoints (Chung et al, 2017a;Darmanis et al, 2017;Lambrechts et al, 2018a;Patel et al, 2014a;Tirosh et al, 2016a;Wang et al, 2019;Zhang et al, 2019a). This is due, in part, to challenges associated with obtaining high-quality biopsies from metastatic tumors, instead of the larger tumor specimens that can be obtained from surgically resected earlier-stage disease.…”

ROGUE: an entropy-based universal metric for assessing the purity of single cell population

“…B cells are key components in tumor microenvironment but have unclear functions in antitumor humoral response 29 . Here we investigated previously reported liver and lung tumor infiltrating B cells 26,30 and found that they received relatively low ROGUE values ( Fig. 4a).…”

“…Pathway activity analysis also revealed a strong antigen processing and presentation signal in this subset (Supplementary Table 4). The fourth cluster, B_C3_FCER2, characterized by high expression of HVCN1 and genes involved in B cell receptor signaling pathway ( Supplementary Table 4), was largely composed of pre-activated B cells 30 . The fifth cluster, B_C4_MX1, predominantly composed of interferon induced B cells 32 , expressed high levels of MX1, IFI6 and IFI44L.…”