Landscape of meiotic crossovers in Hericium erinaceus

Gǒng, Wànkuí; Song, Xiaoya; Xie, Conghua; Zhou, Yingjun; Zhu, Zuohua; Xu, Chao; Peng, Yu

doi:10.1016/j.micres.2020.126692

Cited by 3 publications

(10 citation statements)

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“…Here, in L. edodes, there was a sizeable proportion of progenies that did not show crossover, which was in line with Zymoseptoria tritici [28]. The occurrence of achiasmatic chromosomes was also observed in mushroom-forming basidiomycetes, such as Hericium erinaceus [19] and Pleurotus ostreatus [29]. Overall, the low number of crossovers may limit the efficiency of the breeding process [4].…”

Section: Distribution Of Crossovers In Haploid Progeniesmentioning

confidence: 53%

“…In most eukaryotes, crossovers are non-uniformly distributed along chromosomes and typically cluster within hotspots [32]. It has been widely reported in fungi that crossovers preferentially located near telomeres and sub-telomeric regions [12,19,28,29]. Here, in L. edodes, we recorded crossovers in non-overlapping 10 kb segments and identified hotspot regions with large numbers of crossovers (Figure 1).…”

Section: Crossover Hotspots and Mating Typementioning

confidence: 56%

“…The number of crossovers per SSI ranged from 4 to 16, showing continuous distribution, and suggesting their nature of quantitative traits (Supplementary Figure S1). Comparatively, the number of crossovers per individual in L. edodes (average 8.04) was on the same order of magnitude as other heterothallic basidiomycetes, such as 10.4 in H. erinaceus [19] and 4.2 in S. commune [11], but larger than the homothallic basidiomycete A. bisporus, in which only an average of 0.5 crossovers per individual was observed [12]. Similar to H. erinaceus [19], the number of crossovers was related to the chromosome length, and long chromosomes tended to have a higher number of average crossovers (Supplementary Table S2).…”

Section: Distribution Of Crossovers In Haploid Progeniesmentioning

confidence: 79%

“…A python script "findCrossover.py" (Supplementary File S1) was written to count the number of crossover events per chromosome for each individual. To reduce the influence of mis-genotyped and misplaced markers on identification of crossovers, one crossover event was recorded in a case where at least five consecutive markers on both sides of the recombination breakpoint were from different parents [19]. Meiotic crossovers do not occur uniformly along the chromosomes.…”

Section: Identification Of Crossovers and Hotspotsmentioning

confidence: 99%

“…Moreover, to avoid false crossover detection, a rigorous criterion for haplotype switches was employed. We define a crossover as a change in parental genotype phase for at least five consecutive markers [19].…”

Section: Distribution Of Crossovers In Haploid Progeniesmentioning

confidence: 99%

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Chromosome-Wide Characterization of Intragenic Crossover in Shiitake Mushroom, Lentinula edodes

Gǒng

Shen

Zhang

et al. 2021

JoF

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Meiotic crossover plays a critical role in generating genetic variations and is a central component of breeding. However, our understanding of crossover in mushroom-forming fungi is limited. Here, in Lentinula edodes, we characterized the chromosome-wide intragenic crossovers, by utilizing the single-nucleotide polymorphisms (SNPs) datasets of an F1 haploid progeny. A total of 884 intragenic crossovers were identified in 110 single-spore isolates, the majority of which were closer to transcript start sites. About 71.5% of the intragenic crossovers were clustered into 65 crossover hotspots. A 10 bp motif (GCTCTCGAAA) was significantly enriched in the hotspot regions. Crossover frequencies around mating-type A (MAT-A) loci were enhanced and formed a hotspot in L. edodes. Genome-wide quantitative trait loci (QTLs) mapping identified sixteen crossover-QTLs, contributing 8.5–29.1% of variations. Most of the detected crossover-QTLs were co-located with crossover hotspots. Both cis- and trans-QTLs contributed to the nonuniformity of crossover along chromosomes. On chr2, we identified a QTL hotspot that regulated local, global crossover variation and crossover hotspot in L. edodes. These findings and observations provide a comprehensive view of the crossover landscape in L. edodes, and advance our understandings of conservation and diversity of meiotic recombination in mushroom-forming fungi.

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