Langerhans Cells and Epithelial Cell Modifications in Cervical Intraepithelial Neoplasia: Correlation with Human Papillomavirus Infection

Viac, J.; Guérin-Reverchoni, I.; Chardonnet, Y; Brémond, A.

doi:10.1016/s0171-2985(11)80296-2

Cited by 65 publications

(36 citation statements)

References 39 publications

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“…As most pre-neoplastic lesions of the cervix are associated with a significant depletion of intra-epithelial Langerhans cells, when compared with normal cervix epithelium [8,9,11], we also investigated the migration of in vitro generated DC in the presence of keratinocytes derived from normal cervix and HPV-transformed cell lines in organotypic cultures. We and other workers have observed that HPV + keratinocytes cell lines produce less GM-CSF than normal keratinocytes [29].…”

Section: Discussionmentioning

confidence: 99%

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The organotypic culture of HPV-transformed keratinocytes: an effective in vitro model for the development of new immunotherapeutic approaches for mucosal (pre)neoplastic lesions

Delvenne

Hubert

Jacobs

et al. 2001

Vaccine

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The purpose of this study is to develop a reliable in vitro human model to test new immunotherapeutic approaches for squamous cell carcinoma that develop on mucosal surfaces. The organotypic (raft) culture permits cells to proliferate and differentiate at an air-liquid interface on a dermal equivalent support. Normal keratinocytes stratify and fully differentiate in a manner similar to the normal squamous epithelial tissues, while human papillomavirus-immortalized and established squamous carcinoma cell lines exhibit dysplastic morphologies similar to (pre)neoplastic lesions seen in vivo. We have demonstrated the ability of these organotypic cultures to be manipulated by altering the epithelial stratification with cytokines (interferon-g and tumor necrosis factor-a) and by integrating activated lymphocytes or dendritic cells into the in vitro formed epithelial sheet. This model may provide a useful tool to investigate the factors contributing to the presence and function of immunocompetent cells within a neoplastic epithelium that develops on a mucosal surface.

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Section: Discussionmentioning

confidence: 99%

“…Specific CTL against HPV have been detected in T lymphocytes infiltrating cervical lesions, but only in a minority of patients [7]. Professional antigen-presenting cells, such as Langerhans cells, also exhibit quantitative and qualitative alterations in SILs [8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

The organotypic culture of HPV-transformed keratinocytes: an effective in vitro model for the development of new immunotherapeutic approaches for mucosal (pre)neoplastic lesions

Delvenne

Hubert

Jacobs

et al. 2001

Vaccine

View full text Add to dashboard Cite

show abstract

“…Investigations of cervical biopsies revealed that the number of LCs was significantly reduced in intraepithelial neoplasia and low-grade and high-grade lesions compared to normal tissue results (2,11,29,70). Thus, areas of HPV persistence exhibiting decreases in LC density suggest decreased immune surveillance.…”

mentioning

confidence: 99%

Decreased Migration of Langerhans Precursor-Like Cells in Response to Human Keratinocytes Expressing Human Papillomavirus Type 16 E6/E7 Is Related to Reduced Macrophage Inflammatory Protein-3α Production

Guess¹,

McCance

2005

J Virol

132

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Human papillomaviruses (HPVs) are small DNA viruses which exhibit tropism for cutaneous or mucosal epithelium. Commonly categorized as either low-or high-risk HPV types on the basis of their oncogenic potential, infections with the high-risk HPV types such as HPV-16 and HPV-18 have been implicated in cervical-anogenital cancer and oral squamous cell carcinomas (41). The mucosal lesions caused by HPVs often resolve over time, and a high percentage of young women exposed to HPV clear the infection within a short period following initial contact with the virus (62). Empirical evidence for the critical role the immune response plays in controlling an HPV infection comes from the high prevalence and persistence of HPV infections in immunosuppressed individuals (62). In general, the mucosal immune response to an infection begins with the cells of the epidermis secreting alarm signals which act on immune cells as well as the infected cells.

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“…Investigations of cervical biopsies revealed that the number of LCs was significantly reduced in intraepithelial neoplasia and low-grade/high-grade lesions when compared to normal tissue (2,11,70). Thus, areas of HPV persistence exhibiting decreases in LC density suggest decreased immune surveillance.…”

mentioning

confidence: 99%

Decreased Migration of Langerhans Precursor-Like Cells in Response to Human Keratinocytes Expressing HPV-16 E6/E7 is Related to Reduced Macrophage Inflammatory Protein-3Alpha Production

Guess¹,

McCance²

2005

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Langerhans Cells and Epithelial Cell Modifications in Cervical Intraepithelial Neoplasia: Correlation with Human Papillomavirus Infection

Cited by 65 publications

References 39 publications

The organotypic culture of HPV-transformed keratinocytes: an effective in vitro model for the development of new immunotherapeutic approaches for mucosal (pre)neoplastic lesions

The organotypic culture of HPV-transformed keratinocytes: an effective in vitro model for the development of new immunotherapeutic approaches for mucosal (pre)neoplastic lesions

Decreased Migration of Langerhans Precursor-Like Cells in Response to Human Keratinocytes Expressing Human Papillomavirus Type 16 E6/E7 Is Related to Reduced Macrophage Inflammatory Protein-3α Production

Decreased Migration of Langerhans Precursor-Like Cells in Response to Human Keratinocytes Expressing HPV-16 E6/E7 is Related to Reduced Macrophage Inflammatory Protein-3Alpha Production

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