Langmuir and Langmuir−Blodgett Films of Organophosphorus Acid Anhydrolase

Mello, Sarita V.; Mabrouki, Mustapha; Cao, Xin; Leblanc, Roger M.; Cheng, Thomas K.; DeFrank, Joseph

doi:10.1021/bm025775+

Cited by 18 publications

(20 citation statements)

References 29 publications

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“…The molecularscale LB technique has been proposed for the preparation of biosensors using bioactive molecules (e.g., antibodies and enzymes) as the biorecognition elements (Table 5). 203 OPAA rearranged during the enzymic hydrolysis of DFP, leading to self-quenching of FITC fluorescence in the FITC-OPAA film. Enzymes can be adequately immobilized in an organized ultra-thin monolayer while their biological activities were retained.…”

Section: Luminescent Lb Film Biosensorsmentioning

confidence: 99%

“…The LB monolayer of FITC-labeled enzyme organophosphorus acid anhydrolase (OPAA) was prepared for the detection of diisopropylfluorophosphate (DFP). 203 OPAA rearranged during the enzymic hydrolysis of DFP, leading to self-quenching of FITC fluorescence in the FITC-OPAA film. A linear relationship was observed between the emission intensity of the film and the concentration of DFP in the range of 1.0-10 nM.…”

Section: Luminescent Lb Film Biosensorsmentioning

confidence: 99%

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Luminescent films for chemo- and biosensing

et al. 2015

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Luminescent films have received great interest for chemo-/bio-sensing applications due to their distinct advantages over solution-based probes, such as good stability and portability, tunable shape and size, non-invasion, real-time detection, extensive suitability in gas/vapor sensing, and recycling. On the other hand, they can achieve selective and sensitive detection of chemical/biological species using special luminophores with a recognition moiety or the assembly of common luminophores and functional materials. Nowadays, the extensively used assembly techniques include drop-casting/spin-coating, Langmuir-Blodgett (LB), self-assembled monolayers (SAMs), layer-by-layer (LBL), and electrospinning. Therefore, this review summarizes the recent advances in luminescent films with these assembly techniques and their applications in chemo-/bio-sensing. We mainly focused on the discussion of the relationship between the sensing properties of the films and their architecture. Furthermore, we discussed some critical challenges existing in this field and possible solutions that have been or are being developed to overcome these challenges.

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Section: Luminescent Lb Film Biosensorsmentioning

confidence: 99%

Section: Luminescent Lb Film Biosensorsmentioning

confidence: 99%

Luminescent films for chemo- and biosensing

et al. 2015

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“…It has the capability, similarly to OPH, to hydrolyze a wide range of organophosphates (OPs) with P-F, P-O, P-CN, and P-S bonds. 181,217 It has preferential activity toward P-F bonds and minimal activity towards P-O or P-S bonds, and as such is the enzyme of choice for destroying fluorine-containing organophosphates, such as Sarin and Soman. Diisopropylfluorophosphate (DFP) is a phosphonofluoridate neurotoxin similar in structure to Sarin and Soman with a significantly reduced toxicity, which makes it an ideal analogue for chemical warfare agents.…”

Section: Organophosphate Hydrolasementioning

confidence: 99%

Biosensor technology: recent advances in threat agent detection and medicine

et al. 2013

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Biosensors are of great significance because of their capability to resolve a potentially large number of analytical problems and challenges in very diverse areas such as defense, homeland security, agriculture and food safety, environmental monitoring, medicine, pharmacology, industry, etc. The expanding role of biosensing in society and a real-world environment has led to an exponential growth of the R&D efforts around the world. The world market for biosensor devices, according to Global Industry Analysts, Inc., is expected to reach $12 billion by 2015. Such expedient growth is driven by several factors including medical and health problems, such as a growing population with a high risk of diabetes and obesity, and the rising incidence of chronic diseases such as heart disease, stroke, cancer, chronic respiratory diseases, tuberculosis, etc.; significant problems with environmental monitoring; and of course serious challenges in security and military applications and agriculture/food safety. A review paper in the biosensor technology area may be structured based on (i) the principles of detection, such as the type of transducer platform, bioanalytical principles (affinity or kinetic), and biorecognition elements origin/properties (i.e. antibodies, enzymes, cells, aptamers, etc.), and (ii) the application area. This review follows the latter strategy and focuses on the applications. This allows discussion on how different sensing strategies are brought to bear on the same problem and highlights advantages/disadvantages of these sensing strategies. Given the broad range of biosensor related applications, several particularly relevant areas of application were selected for review: biological threat agents, chemical threat agents, and medicine.

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“…To realize this aim, many analytical methods have been developed to detect the presence of OP compounds, e.g., gas chromatography, , high-performance liquid chromatography (HPLC), , and electrical methods of detection . A fast, efficient, and cheap method for the detection of organophosphorus compounds is the use of enzymes such as organophosphorus hydrolase (OPH) and acetylcholinesterase (AChE) as sensors. − In the case of AChE, a nucleophilic attack of the phosphorus atom from the paraoxon at the serine site which is part of the binding site of AChE and activated by the imidazole group of a histidine explained the affinity of the detection. , Because of the high activity of serine at the binding site and the microenvironment in the pocket of AChE, this reaction is highly efficient.…”

Section: Introductionmentioning

confidence: 99%

Peptidolipid as Binding Site of Acetylcholinesterase: Molecular Recognition of Paraoxon in Langmuir Films

Wang

et al. 2006

Langmuir

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Peptidolipid C18H35O (stearoyl)-Phe-Trp-Ser-His-Glu (peptidolipid A) was synthesized and spread at the air-water interface to study the interaction with an organophosphorus compound. Paraoxon, sodium dihydrogen phosphate, or 4-nitrophenyl phosphate disodium was added to the subphase, but only paraoxon changed the surface pressure-area (pi-A) isotherm of peptidolipid A. This indicated a specific interaction between paraoxon and peptidolipid A. To clarify which amino acid residue of peptidolipid A was responsible for the interaction, peptidolipid B, namely, C18H35O-Gly-His-Ser-Glu-Glu, was synthesized and studied as a Langmuir film. The difference between the pi-A isotherms of peptidolipid B in the absence and presence of paraoxon in the subphase was minimal; consequently, the presence of amino acids phenylalanine (Phe) and tryptophan (Trp) in peptidolipid A may explain the interaction between peptidolipid A and paraoxon. The compression-decompression cycles and kinetic studies of peptidolipid A showed that the Langmuir film was stable. The in situ optical properties of the peptidolipid A Langmuir film such as UV-vis and fluorescence spectroscopies were examined to elucidate the interaction between peptidolipid A and paraoxon. UV-vis absorption of peptidolipid A was investigated in the presence and absence of paraoxon in the subphase. The emission maximum of fluorescence of Trp in peptidolipid A was observed at 351 nm on pure water, and the band intensity decreased when the concentration of paraoxon increased in the subphase. This suggested that the Trp was involved in the molecular recognition process. Epifluorescence micrographs showed domains of peptidolipid A on the pure water subphase. In the presence of paraoxon in the subphase, the Langmuir film of peptidolipid A showed a homogeneity, which was another indication of the recognition between paraoxon and peptidolipid A.

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Langmuir and Langmuir−Blodgett Films of Organophosphorus Acid Anhydrolase

Cited by 18 publications

References 29 publications

Luminescent films for chemo- and biosensing

Luminescent films for chemo- and biosensing

Biosensor technology: recent advances in threat agent detection and medicine

Peptidolipid as Binding Site of Acetylcholinesterase: Molecular Recognition of Paraoxon in Langmuir Films

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