Langmuir-blodgett films of immunoglobulins as sensing elements

Erokhin, Victor; Kayushina, R. L.; Lvov, Y.; Feĭgin, L. A.

doi:10.1007/bf02450391

Cited by 26 publications

(13 citation statements)

References 6 publications

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“…The two curves display a similar trend, increasing their value with pressure. It is worth noting that the maximum thickness value, reached at 45 mN/m (solid line), corresponds well to the protein size in transmembrane direction …”

Section: Resultsmentioning

confidence: 52%

“…Among the available techniques allowing the formation of protein thin films, the Langmuir−Blodgett (LB) technique seems to be the only which can provide densely packed films with molecular resolution in their thickness and, if necessary, organized superstructures, i.e., alternating layers of functional molecules . This technique has been successfully used for depositing films of different kinds of proteins such as antibodies, − enzymes, − cytochromes, − bacterio- 18,19 and bovine 20 rhodopsins, and photosynthetic reaction centers (RCs) from different bacteria. − In most cases protein functional activity is retained in the films …”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Surface Pressure Induced Structural Effects in Photosynthetic Reaction Center Langmuir-Blodgett Films

Facci¹,

Erokhin²,

Paddeu³

et al. 1998

Langmuir

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The structural modifications of photosynthetic reaction centers from Rhodobacter sphaeroides arranged in Langmuir films and after deposition onto solid substrate have been investigated as well as the variations in protein orientation as a function of surface pressure. While at low surface pressure (15−25 mN/m) protein integrity appears to be affected by the action of the surface tension, as results from the film behavior at the air−water interface and from optical spectroscopy data. When the surface pressure (30−45 mN/m) is increased, protein structure is much better preserved. Moreover, at those deposition pressures, the film is characterized not only by a higher surface density but also by an increased anisotropy, as revealed by polarized absorption spectroscopy and confirmed by optical ellipsometry. Measurements of water content desorption in deposited films by means of a gravimetric transducer have consistently confirmed the preservation of protein structure at higher surface pressures.

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Section: Resultsmentioning

confidence: 52%

Section: Introductionmentioning

confidence: 99%

Surface Pressure Induced Structural Effects in Photosynthetic Reaction Center Langmuir-Blodgett Films

Facci¹,

Erokhin²,

Paddeu³

et al. 1998

Langmuir

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“…Langmuir monolayers, on one hand, mimic biomembrane surfaces and therefore facilitate studies of the interactions with adjacent proteins; on the other hand, they can be used to build up films made of proteins. 118 However, this is difficult to achieve with reasonable precision, because most proteins are not amphiphilic like lipids. In that respect, the LbL technique is more suitable, because proteins can be considered to be charged particles.…”

Section: ■ Multicomponent Filmsmentioning

confidence: 99%

From Langmuir Monolayers to Multilayer Films

Moehwald

Brezesinski

2016

Langmuir

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This feature article is intended to describe a route from Langmuir monolayers as the most suitable and well-defined models to polyelectrolyte multilayers. The latter are structurally controlled not with angstrom but with nanometer precision; however, they are very modular with regard to building blocks and function and are robust, therefore promising many diverse applications. There have been many methods developed to structurally characterize Langmuir monolayers; therefore, they serve as models in membrane biophysics and materials science as well as in general physics as two-dimensional model systems. Many of these methods as well as ideas to control interfaces could be taken over to study polyelectrolyte multilayers with their extended internal interfaces. Finally, as an outlook we try to sketch various aspects to transit toward systems with higher structural hierarchy, enabling the coupling of different functions and arriving at responsive three-dimensional systems.

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“…This is relevant for understanding the laser ablation process (Sokolowski-Tinten et al, 1998), and more generally for understanding the characteristics of motion in correlated plasmas. We reported recently (Rischel et al, 1997) a study of the response of an organic Langmuir±Blodgett (LB) ®lm (Erokhin et al, 1996;Matsuda et al, 1977;Nitz et al, 1996;Prakash et al, 1986;Schwartz, 1997) to ultrashort visible laser excitation. The atomic disorder induced in a lattice within the ®rst picosecond following laser energy deposition was, for the ®rst time, directly shown using X-rays.…”

Section: Introductionmentioning

confidence: 99%

Subpicosecond X-ray diffraction study of laser-induced disorder dynamics above the damage threshold of organic solids

Rousse

Rischel²,

Uschmann

et al. 1999

J Appl Cryst

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Ultrafast disordering in Langmuir±Blodgett (LB) ®lms and TlAP (thallium acid phthalate) crystals has been investigated by optical-pump±X-ray-probe experiments using intense femtosecond laser pulses. A laserproduced plasma X-ray source at 7.12 A Ê wavelength is used to study atomic dynamics by subpicosecond timeresolved X-ray diffraction. It is found that a drop of the X-ray diffracted intensity appears with time constants from below 600 fs up to a few tens of picoseconds in LB ®lms optically excited at laser¯uxes from 1.8 to 27 J cm À2 . This loss in the diffracted intensity is understood as a displacement of the diffracting atoms by $8 A Ê from their equilibrium position. The response of the TlAP crystal was signi®cantly slower. The relation to the atomic structure of the materials is discussed.

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Langmuir-blodgett films of immunoglobulins as sensing elements

Cited by 26 publications

References 6 publications

Surface Pressure Induced Structural Effects in Photosynthetic Reaction Center Langmuir-Blodgett Films

Surface Pressure Induced Structural Effects in Photosynthetic Reaction Center Langmuir-Blodgett Films

From Langmuir Monolayers to Multilayer Films

Subpicosecond X-ray diffraction study of laser-induced disorder dynamics above the damage threshold of organic solids

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