Langmuir-Blodgett Nanotemplate Crystallization Combined to Laser-Microfragmentation Uniquely Characterize Proteins Crystals by Synchrotron Microdiffraction

Abstract

Laser-induced microfragmentation of LB nanotemplate-induced protein crystals in glycerol solution results in distinct, coherently diffracting domains. Only crystals produced according to the Langmuir-Blodgett (LB) nanotemplate technique reveal in all four proteins being tested (lysozyme, insulin, thaumatin and ribonuclease) domains highly radiation resistant, while the crystals produced by the standard hanging drop crystallization method do not. Actually the very same laser exposure causes the disappearance of these "classical" protein crystals during the same time frame of 40 min needed for the laser cutting in all four proteins being tested. The microdiffraction of microcrystals prepered by the combination of LangmuirBlodgett and Laser technologies proves that not only the Lysozyme survives the process, as shown recently by nanodifraction, but also all three other model proteins appear to behave similarly well, namely insulin, thaumatin and ribonuclease. The result confirms the emerging of a new biophysical technique uniquely usefull for synchrotron radiation studies based on small protein microcrystals uniquely radiation resistant when prepered by LB nanotemplate and subsequently fragmented by Laser.

Science and Technology for A Sustainable Human Development

“…As a results of the above experimentations at the nanobioscale (Figures 1-6) previously reported [1][2][3][4][5][6][7][8][9][10][11], we achieved a great progress in the area of X-ray protein crystallography for Cancer Research. The Langmuir-Blodgett (LB) nanotemplate method, which became applicable in recent times to any protein, including membrane proteins as large protein as Penniciline G Acylase [11], allows highly ordered 2D LB thin protein films formation on the air-water interface and their deposition on the any solid supports, including nano-patterned chip surfaces.…”

“…Invited by the President Segeev of the Russian Academy Sciences, in official partnership with the Russian Peace Foundation and UNESCO in Moscow for the 10 th Anniversary International Forum on "Science for a sustainable human development" held on 26 and 27, November 2018 at the Ritz-Carlton Hotel. With the new generation of Synchrotrons Diffraction of Multilayered Langmuir-Blodgett Penniciline G Acylase film after Heating and Cooling, based on Langmuir Blodgett technology, in combination to laser microfragmentation, of In situ MicroGISAXS along with CryoEM and Atomic Force Microscopy, all shown in figures 1-6 [1][2][3][4][5][6][7][8][9][10][11].…”