Langmuir-Blodgett Technique for Synthesis of Biomimetic Lipid Membranes

Girard-Egrot, Agnès; Blum, Loı̈c J.

doi:10.1007/0-387-37740-9_2

Cited by 61 publications

(62 citation statements)

References 199 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As the monolayer is compressed, a surface pressure (π) versus surface area (A) isotherm is recorded and the phase (or aggregation state) of the lipids at different surface pressures can be determined. The derivative of this curve can be used to find the monolayer elasticity or fluidity [100]. The composition of the monolayer can be tuned, and the packing of the monolayer can be adjusted by varying the surface pressure, allowing the effect of different nanoparticles on the monolayers of different designated properties to be studied.…”

Section: Interactions Between Pamam Dendrimers and Langmuir Monolayersmentioning

confidence: 99%

PAMAM dendrimer - cell membrane interactions

Fox

Richardson

Briscoe

2018

Advances in Colloid and Interface Science

191

135

View full text Add to dashboard Cite

General rightsThis document is made available in accordance with publisher policies. Please cite only the published version using the reference above. 2018 PAMAM dendrimers have been conjectured for a wide range of biomedical applications due to their tuneable physicochemical properties. However, their application has been hindered by uncertainties in their cytotoxicity, which is influenced by dendrimer generation (i.e. size and surface group density), surface chemistry, and dosage, as well as cell specificity. In this review, biomedical applications of polyamidoamine (PAMAM) dendrimers and some related cytotoxicity studies are first outlined. Alongside these in vitro experiments, lipid membranes such as supported lipid bilayers (SLBs), liposomes, and Langmuir monolayers have been used as cell membrane models to study PAMAM dendrimer-membrane interactions. Related experimental and theoretical studies are summarized, and the physical insights from these studies are discussed to shed light on the fundamental understanding of PAMAM dendrimer-cell membrane interactions. We conclude with a summary of some questions that call for further investigations.a b s t r a c t a r t i c l e i n f o Available online 27 June

show abstract

Section: Interactions Between Pamam Dendrimers and Langmuir Monolayersmentioning

confidence: 99%

PAMAM dendrimer - cell membrane interactions

Fox

Richardson

Briscoe

2018

Advances in Colloid and Interface Science

191

135

View full text Add to dashboard Cite

show abstract

“…The initially formed ternary complexes between DPPC headgroups, metal cations and DNA are not in a thermodynamic equilibrium (Fig. 1) phases co-exist in these time intervals (Girard-Ergot and Blum 2007). Thermodynamic equilibrium is reached in much longer times (Fig.…”

Section: Langmuir Monolayersmentioning

confidence: 98%

“…The rectangular trough and the barrier were made of Teflon. The surface pressure (p) was measured by the Wilhelmy method using a filter paper plate (Stine and Moore 2002;Girard-Ergot and Blum 2007). The measurement is based on the determination of force due to surface tension on a plate suspended partially in the subphase.…”

Section: Lipid Monolayer Formation and Surface Pressure Measurementsmentioning

confidence: 99%

Supramolecular Langmuir monolayers and multilayered vesicles of self-assembling DNA–lipid surface structures and their further implications in polyelectrolyte-based cell transfections

2015

View full text Add to dashboard Cite

The basic interfacial characteristics of DNA-lipid recognitions have been studied. The complex structures of individual unbound DNA molecules and their binary and ternary complexes with zwitterionic lipids and divalent cations were followed by employing lipid monolayers at the air-liquid interfaces, as well as by performing various microscopic, spectroscopic, and thermodynamic measurements with multilayered vesicles. The pressure-area isotherms depicted that Mg 2? -ions increase the surface pressure of lipid films and thus give rise to electrostatic and hydrophobic lipid-DNA interactions in terms of DNA adsorption, adhesion, and compaction. These features were further approached by using multilamellar vesicles with a mean diameter of 850 nm, where a metal ion-directed nucleic acid compaction and condensation effects were shown. The data obtained show the effectiveness of Langmuir monolayers and lipid multilayers in studying nucleic acid-lipid recognitions. The data provide with further details and support previous reports on mainly structural features of these recognitions. Biomolecular surface recognition events were presented in direct link with spectral and thermodynamic features of lipid vesicle-polynucleotide complex formations. The results serve to build a theoretical model considering the use of neutral lipids in lipoplex designs as a polyelectrolyte alternatives to the currently employed cytotoxic cationic liposomes. The supramolecular structures formed and their possible roles in interfacial electrostatic and hydrophobic mechanisms of endosomal escape in relevant cell transfection assays are particularly emphasized.

show abstract

“…Phase states of monolayers depend on Van der Waals forces and intermolecular interactions between the polar groups of molecules. [16,17] Isotherms of compression of amphiphilic polybetaines monolayers on the water-air surface are shown on Figure 7. The curves were recorded after 30 min from the moment of spreading of solution onto subphase.…”

Section: Tem Measurementsmentioning

confidence: 99%

Physicochemical Properties of Hydrophobically Modified Polymeric Betaines and of Their Langmuir‐Blodgett Films

Seliverstova

Ibrayev

Shakhvorostov

et al. 2016

Macromolecular Symposia

View full text Add to dashboard Cite

H 37 ) were synthesized by Michael addition reaction of alkylaminocrotonates and methacrylic acid. They were characterized by FTIR, 13 C NMR, DLS, TEM, viscometry and zeta-potential measurements. In aqueous KOH and DMSO solutions the hydrophobically modified polymeric betaines behave polyelectrolyte character. The average hydrodynamic size and zeta potential of diluted aqueous solutions of hydrophobic polybetaines containing tetradecyl-and octadecyl groups were studied as a function of pH. Anomalous low values of the isoelectric point (IEP) of amphoteric macromolecules were found to be in the range of pH 2.7-3.4. The TEM results revealed that in both aqueous KOH and DMSO media the nano-and microsized micelles and vesicles are existed. The structural organization of micelles and vesicles in water and water À DMSO mixture is discussed. Langmuir-Blodgett (LB) monolayers on the water-air interface were obtained by spreading of hydrophobic polybetaines containing tetradecyl-and octadecyl groups dissolved in THF on the subphase surface. The LB films on the subphase surface are characterized by the specific areas of 0.6 AE 0.05 nm 2 Á monomeric unit À1 at collapsed state. In the spectra of LangmuirBlodgett film the absorption band with the maximum at 255 and 270 nm was observed.

show abstract

Langmuir-Blodgett Technique for Synthesis of Biomimetic Lipid Membranes

Cited by 61 publications

References 199 publications

PAMAM dendrimer - cell membrane interactions

PAMAM dendrimer - cell membrane interactions

Supramolecular Langmuir monolayers and multilayered vesicles of self-assembling DNA–lipid surface structures and their further implications in polyelectrolyte-based cell transfections

Physicochemical Properties of Hydrophobically Modified Polymeric Betaines and of Their Langmuir‐Blodgett Films

Contact Info

Product

Resources

About