Lanthanide-based luminescent assays for ligand-receptor interactions

Handl, Heather L.; Gillies, Robert J.

doi:10.1016/j.lfs.2005.01.009

Cited by 101 publications

(81 citation statements)

References 44 publications

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“…The majority of the studies involving lanthanide(III) complexes as luminescent bio-markers have dealt with thermodynamically stabilized complexes of anionic polychelating ligands such as b-diketones, polycarboxylates (EDTA, DTPA), aromatic amine derivates (pyridine, bipyridine, terpyridine, phenanthroline) or macrocycles. [126][127][128] A general structure of typical fluorescent lanthanide chelates for bioanalytical assays is shown in Fig. 8b, that consists on a terpyridine group and complexing polycarboxylates.…”

Section: Lanthanide-doped Hybrid Materials As Sensors In Biological Mmentioning

confidence: 99%

Photonic and nanobiophotonic properties of luminescent lanthanide-doped hybrid organic–inorganic materials

et al. 2008

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Research into lanthanide-doped organic-inorganic hybrid materials emerged in the 1990s with the development of interesting materials for optics: high efficiency and stable solid-state lasers, new fiber amplifiers and sensors, devices with upconversion, fast photochromic and non-linear responses, etc. Their interest relies on the possibility of combining properties of sol-gel host materials (shaping, tunable refractive index and mechanical properties, corrosion protection, specific adhesion, etc.) and the well-known luminescence of lanthanide ions (Ln). The fast development of photonic hybrids allowed the commercial exploitation of products with new or enhanced characteristics (megajoule pulsed Nd-YAG laser, protective coatings of glasses, screens or glasswares). However, recently, Ln-hybrid nanocomposites have found new applications in bio-sensors, bio-analytics and even clinical imaging diagnostics. These applications make use of the fluorescence properties of lanthanides that make luminescent hybrids ideal candidates for time-resolved fluoroimmunoassays, DNA hybridation assays, fluorescence imaging microscopy, or in vivo imaging. As a consequence, the goal of this review is twofold: (i) as a reminder of some general considerations that must be taken into account to design new optically active Ln-doped nanocomposites whatever the application field, and (ii) to show the most important advances achieved in the past years in different areas, paying special attention to bio-medical applications.

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Section: Lanthanide-doped Hybrid Materials As Sensors In Biological Mmentioning

confidence: 99%

Photonic and nanobiophotonic properties of luminescent lanthanide-doped hybrid organic–inorganic materials

et al. 2008

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“…4 Specifically, their sharp 'atom-like' emission spectra, large Stokes shift, and long lived luminescence makes trivalent lanthanide cations such as Eu(III) very attractive reporters for these assays. Using both spectral and temporal discrimination of the luminescent signal from background autofluorescence enables highly sensitive assays, 5,6 such as the Dissociation Enhanced Lanthanide Fluoro Immuno Assay (DELFIA ® ) techniques first commercialized by Wallac ™ , which use a polyaminocarboxylate chelate of Eu(III). 7 However, the DELFIA ® format is heterogeneous and suffers from the limitation that the fluorescent signal cannot be traced until the end of the assay, when the non-fluorescent Ln(III) chelate is converted to a highly fluorescent form by the addition of a sensitizing -diketonate enhancement solution.…”

Section: Introductionmentioning

confidence: 99%

Highly Luminescent Lanthanide Complexes of 1-Hydroxy-2-pyridinones

et al. 2008

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The synthesis, X-ray structure, stability, and photophysical properties of several trivalent lanthanide complexes formed from two differing bis-bidentate ligands incorporating either alkyl or alkyl ether linkages and featuring the 1-hydroxy-2-pyridinone (1,2-HOPO) chelate group in complex with Eu(III), Sm(III) and Gd(III) are reported. The Eu(III) complexes are among some of the best examples, pairing highly efficient emission ( Eu tot Φ ~ 21.5 %,) with high stability (pEu ~ 18.6) in aqueous solution, and are excellent candidates for use in biological assays. A comparison of the observed behavior of the complexes with differing backbone linkages shows remarkable similarities, both in stability and photophysical properties. Low temperature photophysical measurements for a Gd(III) complex were also used to gain insight into the electronic structure, and were found to agree with corresponding TD-DFT calculations for a model complex. A comparison of the high resolution Eu(III) emission spectra in solution and from single crystals also revealed a more symmetric coordination geometry about the metal ion in solution due to dynamic rotation of the observed solid state structure.

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“…However, when the lanthanide ions form chelates with organic ligands, the complex exhibits unique fluorescent properties, such as a sharp emission band (typically 10-20 nm), large Stokes shift, long emission lifetimes compared to the nanosecond range for traditional organic reagents. The long-lived luminescence offers a signal-to-noise ratio advantage and improved signal sensitivity, which enables the use of time-resolved fluorescent (TRF) spectroscopy (Diamandis 1988;Handl and Gillies 2005;Josan et al 2011). Taking advantage of the physical properties of lanthanide chelates, in combination with a dissociation-based enhancement assay called DELFIA (dissociation enhanced lanthanide fluoroimmunoassay), we have developed a robust binding assay for the detection of ligands binding to RXFP3, using time-resolved fluorescence measurement.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and pharmacological characterization of a europium-labelled single-chain antagonist for binding studies of the relaxin-3 receptor RXFP3

et al. 2015

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Relaxin-3 and its endogenous receptor RXFP3 are involved in fundamental neurological signalling pathways, such as learning and memory, stress, feeding and addictive behaviour. Consequently, this signalling system has emerged as an attractive drug target. Development of leads targeting RXFP3 relies on assays for screening and ligand optimization. Here, we present the synthesis and in vitro characterization of a fluorescent europium-labelled antagonist of RXFP3. This ligand represents a cheap and safe but powerful tool for future mechanistic and cell-based receptor-ligand interaction studies of the RXFP3 receptor.

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Lanthanide-based luminescent assays for ligand-receptor interactions

Cited by 101 publications

References 44 publications

Photonic and nanobiophotonic properties of luminescent lanthanide-doped hybrid organic–inorganic materials

Photonic and nanobiophotonic properties of luminescent lanthanide-doped hybrid organic–inorganic materials

Highly Luminescent Lanthanide Complexes of 1-Hydroxy-2-pyridinones

Synthesis and pharmacological characterization of a europium-labelled single-chain antagonist for binding studies of the relaxin-3 receptor RXFP3

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