Laparoscopic Appendectomy as an Alternative to Conventional Procedure – Results in our own Material

Merenda, Marcin; Litarski, Andrzej; Kabziński, Piotr; Janczak, Dariusz

doi:10.2478/pjs-2013-0048

Cited by 3 publications

(4 citation statements)

References 9 publications

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“…We believe that parts of the omental tissue can be harvested from patients in a relatively simple procedure using microsurgery techniques [42,43]. After a quick decellularization process, the obtained matrix would be able to serve as an autologous scaffold for growing tissues composed of cells isolated from the same patient.…”

Section: Omentum Matrix As a Platform For Engineering Autologous Tissuesmentioning

confidence: 99%

“…During many clinical procedures the omentum is removed from patients without health implications [40,41]. Therefore, we believe that parts of this tissue can be removed by relatively simple microsurgery techniques [42,43] and further processed to serve as autologous matrices. Such platform can be utilized for engineering personalized cardiac patches.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Fabrication of omentum-based matrix for engineering vascularized cardiac tissues

Shevach¹,

Soffer-Tsur²,

Fleischer³

et al. 2014

Biofabrication

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Fabricating three-dimensional, biocompatible microenvironments to support functional tissue assembly remains a key challenge in cardiac tissue engineering. We hypothesized that since the omentum can be removed from patients by minimally invasive procedures, the obtained underlying matrices can be manipulated to serve as autologous scaffolds for cardiac patches. Here we initially characterized the structural, biochemical and mechanical properties of the obtained matrix, and demonstrated that cardiac cells cultivated within assembled into elongated and aligned tissues, generating a strong contraction force. Co-culture with endothelial cells resulted in the formation of blood vessel networks in the patch without affecting its function. Finally, we have validated that omental scaffolds can support mesenchymal and induced pluripotent stem cells culture, thus may serve as a platform for engineering completely autologous tissues. We envision that this approach may be suitable for treating the infarcted heart and may open up new opportunities in the broader field of tissue engineering and personalized regenerative medicine.

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Section: Omentum Matrix As a Platform For Engineering Autologous Tissuesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Fabrication of omentum-based matrix for engineering vascularized cardiac tissues

Shevach¹,

Soffer-Tsur²,

Fleischer³

et al. 2014

Biofabrication

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“…Since the first open appendectomy in 1894 credited to McBurney, for more than a century, it has remained unchanged [ 2 ]. It was until 1983 that Semm described the first laparoscopic appendectomy, quickly becoming the gold standard in complicated and uncomplicated appendicitis [ [2] , [3] , [4] , [5] ]. An incidence of 100/100 000 cases per year is reported, with an 8% lifetime risk of developing appendicitis [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Zaragoza technique of laparoscopic appendicectomy

Flores

Ávila

Cruz

et al. 2021

Annals of Medicine &Amp; Surgery

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Introduction Appendectomy is one of the most frequent emergency surgical procedures, currently with a preference for laparoscopic management worldwide. Objective To report a new laparoscopic appendectomy technique and its results. Material and methods Cohort study of patients with a diagnosis of appendicitis who are managed laparoscopically. In a total 1063 patients, 148 were operated on with the Zaragoza technique during the period from January 2002 to December 2018. The technique consists of making a window in the appendicular base between the meso and the appendicular wall, two prolene or silk sutures are placed, and the cecal appendix is cut between the two sutures, finally the mesoappendix is sectioned with a harmonic scalpel or bipolar clamp. Results From our results, we had 1.4% residual abscesses, 1.4% umbilical surgical wound infection and 0% mortality. Discussion Various laparoscopic management methods for appendectomy are reported in the literature, with a wide range in the results. We have obtained good results in patients subjected to our technique. Conclusions The Zaragoza technique for laparoscopic appendectomy is an effective and safe option that prevents excessive manipulation of the inflamed appendix and is easily reproducible.

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“…Recently, we reported on the utilization of the omental matrix as a platform for engineering autologous functional cardiac patches [33,34]. The omentum, a double sheet of highly vascularized peritoneum, can be easily, quickly and safely extracted from patients without health implications [35][36][37] and the processed biomaterial can be implanted without provoking an inflammatory response. In this study, we further processed the decellularized omental matrix to serve as thermoresponsive hydrogel for cell delivery.…”

Section: Introductionmentioning

confidence: 99%

Omentum ECM-based hydrogel as a platform for cardiac cell delivery

Shevach¹,

Zax²,

Abrahamov³

et al. 2015

Biomed. Mater.

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Cardiovascular diseases remain the number one killer in Western countries. Despite recent advances and promising results in cardiac cell-based therapy, one of the remaining challenges is poor cell retention in the desired site. As a solution, cell delivery systems are developed to ensure that a sufficient number of viable cells reach the infarct area. These delivery systems are based on biomaterials that provide a surrogate microenvironment for the encapsulated cells, retaining them in the desired location post-delivery. Injectable thermoresponsive ECM-based hydrogels have been developed to achieve this goal. Unfortunately, the use of allogeneic or xenogeneic ECM may hamper the treatment due to an immune response to residual cellular content from the host. In this work, we have developed an omentum-based hydrogel capable of self-assembly under physiological conditions. Although in this study the omentum was obtained from porcine sources, it can be easily and safely extracted from the patient, serving as an autologous protective vehicle for the transported cells. We have characterized the biochemical composition, mechanical properties, and gelation and degradation kinetics of the processed biomaterial. Furthermore, the ability of the hydrogel to encapsulate cardiac cells and support their culture was evaluated. We envision that the newly developed platform may open new opportunities for personalized cell delivery to the heart and other tissues.

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Laparoscopic Appendectomy as an Alternative to Conventional Procedure – Results in our own Material

Cited by 3 publications

References 9 publications

Fabrication of omentum-based matrix for engineering vascularized cardiac tissues

Fabrication of omentum-based matrix for engineering vascularized cardiac tissues

Zaragoza technique of laparoscopic appendicectomy

Omentum ECM-based hydrogel as a platform for cardiac cell delivery

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