Laparoscopic inguinal herniorrhaphy facilitated by the use of self-expanding mesh

Steinberg, Scott D.

doi:10.1016/s0002-9270(01)03654-1

Cited by 1 publication

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“…Tests for low abundance biomarkers such as human prostate specific antigen (PSA) and human pancreatic and gastrointestinal cancer marker (CA 19-9) are routinely used for initial tumor screening, to monitor patient response during cancer therapy, and to help detect recurrence following treatment2, 3. Therefore, there is significant clinical relevance to increase the sensitivity of these tests to facilitate early detection of the onset and recurrence of cancer, since these biomarkers get diluted significantly in blood, especially in early stages of the disease progression.…”

Section: Introductionmentioning

confidence: 99%

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Increasing the Sensitivity of Enzyme-Linked Immunosorbent Assay Using Multiplexed Electrokinetic Concentrator

Cheow

Kim

et al. 2010

Anal. Chem.

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We developed a novel method to increase the sensitivity of standard Enzyme-Linked Immunosorbent Assay (ELISA) using a multiplexed electrokinetic concentration chip. The poly(dimethylsiloxane) (PDMS) molecular concentrator 1 was used to trap and collect charged fluorescent product of targetbound enzyme turnover reaction of ELISA that occurred in a standard 96 well plate. Detection sensitivities of both Prostate Specific Antigen (PSA) and CA 19-9 (a human pancreatic and gastrointestinal cancer marker) ELISAs in serum are enhanced ~100 fold with a low CV of <17%. We also integrated this method with an on-chip bead-based ELISA that lends itself toward a fullyautomated on-chip diagnostic device. Detection sensitivity of microfluidic bead-based CA 19-9 ELISA in serum is enhanced ~65 fold compared to the results without electrokinetic accumulation step. This chip can be directly applied to enhance the readout sensitivity of a wide range of existing ELISA kits at concentrations below the current detection limit.

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Section: Introductionmentioning

confidence: 99%

“…Since the same substrate can be used for a whole range of ELISA kits, the same device can be directly applied to immunoassay of different analytes. As a proof of concept, we performed our experiment with two cancer markers, PSA and CA 19-9, which are two important biomarkers in cancer diagnostic and post treatment monitoring2, 3.…”

Section: Introductionmentioning

confidence: 99%

Increasing the Sensitivity of Enzyme-Linked Immunosorbent Assay Using Multiplexed Electrokinetic Concentrator

Cheow

Kim

et al. 2010

Anal. Chem.

View full text Add to dashboard Cite

show abstract

Laparoscopic inguinal herniorrhaphy facilitated by the use of self-expanding mesh

Cited by 1 publication

References 0 publications

Increasing the Sensitivity of Enzyme-Linked Immunosorbent Assay Using Multiplexed Electrokinetic Concentrator

Increasing the Sensitivity of Enzyme-Linked Immunosorbent Assay Using Multiplexed Electrokinetic Concentrator

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