Laparotomy Causes a Transient Induction of Rat Liver Serine Dehydratase mRNA

Ogawa, Hirofumi; Kawamata, Seiichi; Gomi, Tomoharu; Ansai, Yutaka; Karaki, Yoshiaki

doi:10.1006/abbi.1995.1113

Cited by 9 publications

(5 citation statements)

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“…Recently, we found that SD mRNA was transiently induced by laparotomy. The induction by laparotomy was hindered by adrenalectomy or pretreatment with o~-adrenergic blockers, suggesting that glucocorticoids and neural noradrenaline play important roles in the induction process (Ogawa et al, 1995). In rat, sympathetic nerves reach only the outer periportal cells, whereas almost all hepatocytes in guinea pig, rabbit, cat, dog and human have nerve contacts (Jungermann & Katz, 1989).…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, when normal rats were fed ad libitum under a 12-h light (0700-1900)/dark cycle the SD mRNA level in their liver was found to exhibit a diurnal variation with a zenith at the onset of darkness and at the end of the fasting period, and a nadir at the onset of light and at the end of the feeding period (Ogawa et al, 1994). Recently, we found that SD mRNA was transiently induced upon laparotomy, suggesting that the stress releases glucocorticoids and neural noradrenaline that stimulates ~-adrenergic receptors, thereby causing the SD gene expression (Ogawa et al, 1995).…”

Section: Introductionmentioning

confidence: 97%

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Periportal expression of the serine dehydratase gene in rat liver

Ogawa

Kawamata

1995

Histochem J

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The mRNA for rat liver serine dehydratase, a gluconeogenic enzyme, exhibits a circadian rhythm with a maximum at the onset of darkness marking the end of the fasting period and a minimum at the onset of light that marks the end of the feeding period, when rats have free access to food and water. In situ hybridization with an antisense cRNA probe revealed that serine dehydratase mRNA was localized in the periportal area of rat liver parenchyma in the evening, whereas it was scarce in the liver in the morning. The predominant localization of serine dehydratase mRNA in the periportal area also occurred in livers of rats that underwent laparotomy, glucagon and dexamethasone administration, and streptozotocin-induced diabetes mellitus, all of which are known to induce serine dehydratase mRNA levels remarkably. Immunostaining revealed that the localization of serine dehydratase protein agreed with that of succinate dehydrogenase, another enzyme known to be predominant in the periportal zone. Thus, the periportal serine dehydratase gene expression strongly supports the idea of metabolic zonation that gluconeogenesis from amino acids occurs preferentially in the periportal parenchyma of rat liver.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Periportal expression of the serine dehydratase gene in rat liver

Ogawa

Kawamata

1995

Histochem J

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“…Serine-threonine dehydratase was assayed by the method of Ogawa [9]. Briefly, a portion of tissue was homogenised in 2 volumes of buffer (0.15M KCl, 0.1M Tris (pH 7.4) and 1 mM EDTA) and centrifuged at 100 000 × g for 15 min.…”

Section: Serine-threonine Dehydratase Assaymentioning

confidence: 99%

The effect of dietary protein on the amino acid supply and threonine metabolism in the pregnant rat

Rees¹,

Hay²,

Antipatis³

2006

Reprod. Nutr. Dev.

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-To characterise the effects of dietary protein content on threonine metabolism during pregnancy, rats were fed diets containing 18% or 9% protein and then killed at different stages of gestation. Serum threonine concentrations fell significantly faster in the animals fed the diet containing 9% protein when compared to those fed the diet containing 18% protein. On day 4 of gestation the rate of threonine oxidation was higher in maternal liver homogenates prepared from the animals fed the diet containing 18% protein. The rate of threonine oxidation by liver homogenates fell as gestation proceeded in both diet groups. The activity of threonine dehydrogenase in the maternal liver was unaffected by dietary protein content at all stages of gestation. Serine-threonine dehydratase activity in homogenates of the maternal liver was transiently increased during the early stages of gestation in the animals fed high protein diets but was unchanged in the low protein groups. There was an increase in serine-threonine dehydratase activity in the kidney during the later stages of gestation but this was unaffected by the protein content of the maternal diet. These data show that the changes in free threonine concentrations cannot be accounted for through changes in the oxidation rate and suggest that some other factor influences the unusual metabolism of this amino acid during gestation. threonine dehydratase / threonine dehydrogenase / protein metabolism / pregnancy / foetus / metabolic programming Abbreviations: SDH, serine/threonine dehydratase; TDH, threonine dehydrogenase; PCA, perchloric acid; TCA, trichloroacetic acid.

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“…These hormones also regulate the expression of the SDH gene, since the promoter contains a glucocorticoid response element (Su & Pitot, 1992). Indeed, even relatively mild stresses such as laparotomy are able to induce the expression of the enzyme (Ogawa et al 1995). Once changes in SDH activity have been initiated, it is not clear whether they can be restored if the animal is returned to a high-protein diet.…”

Section: Diet Composition and Fetal Growthmentioning

confidence: 99%