LAPS-based monitoring of metabolic responses of bacterial cultures in a paper fermentation broth

Dantism, Shahriar; Röhlen, Désirée; Dahmen, M.; Wagner, Torsten; Wagner, Patrick; Schöning, Michael J.

doi:10.1016/j.snb.2020.128232

Cited by 9 publications

(5 citation statements)

References 50 publications

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“…As a type of field-effect device, compared to a field-effect transistor (FET), LAPS devices with electrolyte-insulator-semiconductor (EIS) structures have advantages including a simple device structure, the ease of modifying sensitive materials, and flexibility in defining detection areas. The insulator material (usually SiO 2 , Si 3 N 4 , and Al 3 O 4 ) of LAPS chips has good biocompatibility and a small sample size requirement and has been successfully applied in the metabolic detection of cells [16,17,[21][22][23][24][25] and bacteria [26][27][28]. However, the applications of LAPS biosensors only include metabolic pH monitoring in a solution environment.…”

Section: Introductionmentioning

confidence: 99%

A Silicon-Based Field-Effect Biosensor for Drug-Induced Cardiac Extracellular Calcium Ion Change Detection

Qiu,

Ma,

Jiang

et al. 2023

Biosensors

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Calcium ions participate in the regulation of almost all biological functions of the body, especially in cardiac excitation–contraction coupling, acting as vital signaling through ion channels. Various cardiovascular drugs exert their effects via affecting the ion channels on the cell membrane. The current strategies for calcium ion monitoring are mainly based on fluorescent probes, which are commonly used for intracellular calcium ion detection (calcium imaging) and cannot achieve long-term monitoring. In this work, an all-solid-state silicone–rubber ion-sensitive membrane was fabricated on light-addressable potentiometric sensors to establish a program-controlled field-effect-based ion-sensitive light-addressable potentiometric sensor (LAPS) platform for extracellular calcium ion detection. L-type calcium channels blocker verapamil and calcium channel agonist BayK8644 were chosen to explore the effect of ion channel drugs on extracellular calcium ion concentration in HL-1 cell lines. Simultaneously, microelectrode array (MEA) chips were employed to probe the HL-1 extracellular field potential (EFP) signals. The Ca2+ concentration and EFP parameters were studied to comprehensively evaluate the efficacy of cardiovascular drugs. This platform provides more dimensional information on cardiovascular drug efficacy that can be utilized for accurate drug screening.

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Section: Introductionmentioning

confidence: 99%

A Silicon-Based Field-Effect Biosensor for Drug-Induced Cardiac Extracellular Calcium Ion Change Detection

Qiu,

Ma,

Jiang

et al. 2023

Biosensors

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“…LAPS offers (depending on its transducer layer) the spatially resolved monitoring of concentration-dependent surface-potential changes, e.g., induced by (bio)chemical/biological molecules or living cells in the analyte solution. LAPS can be designed as multiwell-and multianalyte-sensor devices and can serve for chemical imaging, where the distribution of the analyte concentration is visualized on its chip surface [10][11][12][13][14]. Moreover, LAPS provides a broad range of possible applications and has been utilized for various (bio)chemical and biotechnological approaches, such as monitoring the metabolic activity of bacteria in fermentation broth [14], for on-sensor cryopreservation of cells [15], multi-ion and penicillin detection [16,17], and DNA sensing [18].…”

Section: Introductionmentioning

confidence: 99%

Light-Addressable Actuator-Sensor Platform for Monitoring and Manipulation of pH Gradients in Microfluidics: A Case Study with the Enzyme Penicillinase

et al. 2021

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The feasibility of light-addressed detection and manipulation of pH gradients inside an electrochemical microfluidic cell was studied. Local pH changes, induced by a light-addressable electrode (LAE), were detected using a light-addressable potentiometric sensor (LAPS) with different measurement modes representing an actuator-sensor system. Biosensor functionality was examined depending on locally induced pH gradients with the help of the model enzyme penicillinase, which had been immobilized in the microfluidic channel. The surface morphology of the LAE and enzyme-functionalized LAPS was studied by scanning electron microscopy. Furthermore, the penicillin sensitivity of the LAPS inside the microfluidic channel was determined with regard to the analyte’s pH influence on the enzymatic reaction rate. In a final experiment, the LAE-controlled pH inhibition of the enzyme activity was monitored by the LAPS.

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“…Next to this, sensors also have been developed to monitor the metabolic activity of bacteria so that bacteria, if present, can be identified based on their metabolic products. [ 4,5 ] It is evident that practically all developed biosensors offer a clear advantage over the standard methods in term of measurement time, but there are still shortcomings that need to be tackled. [ 6–8 ] Most of these platforms are only validated in a laboratory environment and often not with “real‐life” samples, obtained from the food industry.…”

Section: Introductionmentioning

confidence: 99%

Low Cost, Sensitive Impedance Detection of E. coli Bacteria in Food‐Matrix Samples Using Surface‐Imprinted Polymers as Whole‐Cell Receptors

Stilman

Lenzi

Wackers

et al. 2021

Physica Status Solidi (a)

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Microbial contaminations are a persistent threat for food safety and it remains difficult to screen food quality onsite in an easy and rapid way. In this work, we report on a low-cost, sensitive, impedance-based biomimetic sensor for the detection of Escherichia coli (E. coli) bacteria in both buffer fluids and, more importantly, in rinse water of an industrial food-processing machine. We have chosen E. coli because this bacterium is considered as a general indicator for hygiene standards and contaminations with other pathogens often go along with the presence of E. coli. [1,2] The identification of the underlying pathogen is time-consuming since cell culturing is always required, often in combination with costly DNA profiling, limiting the use of these techniques for routine screening of food samples. On top of the concerns regarding consumer health, food spoilage by bacteria and the resulting food loss are serious economic issues. [3] Therefore, there is a recent trend to develop low-cost and fast (bio)sensor systems to detect bacterial contaminations timely and directly on-site in the food production chain. Next to this, sensors also have been developed to monitor the metabolic activity of bacteria so that bacteria, if present, can be

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LAPS-based monitoring of metabolic responses of bacterial cultures in a paper fermentation broth

Cited by 9 publications

References 50 publications

A Silicon-Based Field-Effect Biosensor for Drug-Induced Cardiac Extracellular Calcium Ion Change Detection

A Silicon-Based Field-Effect Biosensor for Drug-Induced Cardiac Extracellular Calcium Ion Change Detection

Light-Addressable Actuator-Sensor Platform for Monitoring and Manipulation of pH Gradients in Microfluidics: A Case Study with the Enzyme Penicillinase

Low Cost, Sensitive Impedance Detection of E. coli Bacteria in Food‐Matrix Samples Using Surface‐Imprinted Polymers as Whole‐Cell Receptors

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