Large‐area, high‐resolution image analysis of composite materials

Davidson, N.; Clarke, Ashley; Archenhold, G.

doi:10.1046/j.1365-2818.1997.1560712.x

Cited by 44 publications

(19 citation statements)

References 23 publications

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“…Thus, the residual matrix reorganization observed following sustained Rho kinase inhibition in the current study likely results from localized tractional forces generated during formation and extension of dendritic cell processes. [57]. To define the orientation of a fiber unambiguously in space, two angles are required; in-plane angle φ and out-of-plane angle θ.…”

Section: Discussionmentioning

confidence: 99%

Quantitative assessment of local collagen matrix remodeling in 3-D Culture: The role of Rho kinase

Kim

Lakshman

Petroll

2006

Experimental Cell Research

106

123

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The purpose of this study was to quantitatively assess the role of Rho kinase in modulating the pattern and amount of local cell-induced collagen matrix remodeling.Human corneal fibroblasts were plated inside 100 μm thick fibrillar collagen matrices and cultured for 24 hours in media with or without the Rho kinase inhibitor Y-27632. Cells were then fixed and stained with phalloidin. Fluorescent (for f-actin) and reflected light (for collagen fibrils) 3-D optical section images were acquired using laser confocal microscopy. Fourier transform analysis was used to assess collagen fibril alignment, and 3-D cell morphology and local collagen density were measured using MetaMorph.Culture in serum-containing media induced significant global matrix contraction, which was inhibited by blocking Rho kinase (p < 0.001). Fibroblasts generally had a bipolar morphology and intracellular stress fibers. Collagen fibrils were compacted and aligned parallel to stress fibers and pseudopodia. When Rho kinase was inhibited, cells had a more cortical f-actin distribution and dendritic morphology. Both local collagen fibril density and alignment were significantly reduced (p<0.01).Overall, the data suggests that Rho kinase dependent contractile force generation leads to coalignment of cells and collagen fibrils along the plane of greatest resistance, and that this process contributes to global matrix contraction.

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Section: Discussionmentioning

confidence: 99%

Quantitative assessment of local collagen matrix remodeling in 3-D Culture: The role of Rho kinase

Kim

Lakshman

Petroll

2006

Experimental Cell Research

106

123

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“…Stitching of overlapping single images enables large areas to be studied [8,9]. A stitching code was implemented based on Fast-Fourier correlations [10] programmed in Matlab ® .…”

Section: Materials and Data Acquisitionmentioning

confidence: 99%

Analysis of filament arrangements and generation of statistically equivalent composite micro-structures

Gommer

Endruweit

Long

2014

Composites Science and Technology

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An efficient method to describe and quantify the filament arrangement in fibre bundles based on the analysis of micrographs was developed. Quantitative measurement of relative filament positions indicated that the initially random arrangement of filaments shows increasingly strong characteristics of square and hexagonal configurations with increasing level of transverse compaction. An existing micro-structure generator was extended to incorporate the measured data allowing statistically equivalent filament arrangements to be generated at any fibre volume fraction. These can be used to determine micro-structural properties of actual fibre reinforced composites.

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“…The full characterization of glass and carbon fiber reinforced composites has also been addressed by means of optical microscopy [25] and digital image processing of micrographies has been employed by Joffe and Mattsson [26].…”

Section: Introductionmentioning

confidence: 99%

Quality control of CFRP by means of digital image processing and statistical point pattern analysis

Trias¹,

Garcia²,

Costa³

et al. 2007

Composites Science and Technology

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Although fiber-reinforced composite materials have often been considered as periodic materials in theoretical models, the distribution of fibers is random in real materials. This random distribution of fibers is closely related to their transverse failure behavior. This paper proposes the use of statistical functions which describe random point patterns as a quantification of the dispersion of the transverse failure properties of several carbon fibre reinforced polymers (CFRP). It is shown that the analysis of the K function is the most meaningful for this purpose.

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Large‐area, high‐resolution image analysis of composite materials

Cited by 44 publications

References 23 publications

Quantitative assessment of local collagen matrix remodeling in 3-D Culture: The role of Rho kinase

Quantitative assessment of local collagen matrix remodeling in 3-D Culture: The role of Rho kinase

Analysis of filament arrangements and generation of statistically equivalent composite micro-structures

Quality control of CFRP by means of digital image processing and statistical point pattern analysis

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