Large depth-of-field fluorescence microscopy based on deep learning supported by Fresnel incoherent correlation holography

Wu, Peng; De-jie, Zhang; Yuan, Jing; Zeng, Shaoqun; Gong, Hui; Luo, Qingming; Yang, Xiaoquan

doi:10.1364/oe.451409

Cited by 20 publications

(6 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The diameter of the FL images increased from 12.3 to 29.2 μm as the Z increases from 8 to 30 μm (Table S6). These phenomena should be ascribed to the effect of the depth of the field on FL imaging . During the process of imaging of Ru1-PS@MB, Ru1-PS@MB is the object.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Imaging and Simulation of Ruthenium Derivative Coating Microbeads at the Opaque Electrode with Electrogenerated Chemiluminescence

Feng,

Zhou,

Wang

et al. 2023

Chemical & Biomedical Imaging

View full text Add to dashboard Cite

Electrogenerated chemiluminescence (ECL) imaging is gaining increasing attention in various fields because of its high sensitivity, low background, and good temporal and spatial resolution. However, ECL imaging of microsized objects at the opaque electrode via top-view configuration is challenged with the reactants’ diffusion and light propagation. Here, we imaged and numerically simulated ruthenium derivative coating polystyrene microbeads (Ru1-PS@MB) at the glassy carbon electrode (GCE) via top-view configuration by ECL imaging. The ruthenium derivative (bis(2,2′-bipyridine)-4′-methyl-4-carboxybipyridine-ruthenium N-succinimidyl ester-bis (hexafluorophosphate), Ru1), a typical ECL reagent, was covalently linked onto the surface of aminated PS@MBs via the amide reaction. “Strong emission in edge and weak emission in center” phenomena for fluorescence (FL) and ECL emissions were obtained from Ru1-PS@MB on GCE. Z-Stack imaging of the microsized Ru1-PS@MB luminescence was performed on GCE in the presence of tri-n-propylamine (TPA). It is found that the clear luminescence range of Ru1-PS@MB perpendicular to the electrode surface in ECL image is slightly smaller than that in the FL image. The bigger was the diameter of the microbeads (from 5 to 18 μm), the larger was the ECL luminescence range of Ru1-PS@MB perpendicular to the electrode surface (from 5 to 7 μm). Our findings, which are also supported by numerical simulation, provide insights into the ECL imaging of microsized objects at the electrode surface, which will raise promising ECL applications in bioassays and cell imaging at the microscale level.

show abstract

Section: Resultsmentioning

confidence: 99%

“…These phenomena should be ascribed to the effect of the depth of the field on FL imaging. 37 During the process of imaging of Ru1-PS@MB, Ru1-PS@MB is the object. The Ru1-PS@MB can be clearly recorded only if Ru1-PS@MB is located in the depth of field of the system (d s ).…”

Section: Fl Imagementioning

confidence: 99%

Imaging and Simulation of Ruthenium Derivative Coating Microbeads at the Opaque Electrode with Electrogenerated Chemiluminescence

Feng,

Zhou,

Wang

et al. 2023

Chemical & Biomedical Imaging

View full text Add to dashboard Cite

show abstract

“…After many years of investigations into it, this technology has gained considerable attention, especially in fields like polarization optical imaging, [15][16][17] information encryption, [18,19] 3D displays, [20,21] low coherence imaging, [22,23] and fluorescence microscopic imaging. [24,25] In recent years, OAM holography has been proposed as a vital method for optical encryption in 2019. [26,27] By applying incident OAM beams and spatially sampling a target image, the reconstructed OAMpixelated holographic image can be generated to preserve the OAM property and realize OAM selectivity.…”

Section: Introductionmentioning

confidence: 99%

Superposed Laguerre‐Gaussian Beams‐Based Orbital Angular Momentum Holography

Zeng,

Zhao

2024

Laser & Photonics Reviews

View full text Add to dashboard Cite

Orbital angular momentum (OAM) holography implements OAM of light as an information carrier to reconstruct holographic images, due to a physically unbounded set of orthogonal OAM modes. Here, superposed Laguerre‐Gaussian (LG) beams are employed as incident beams to achieve OAM holography. Notably, this scheme utilizes exact complex‐amplitude modulation, as opposed to mere phase‐only modulation, ensuring the maintenance of amplitude information. In the absence of a matching field, the output images retain the properties of incident beams with a varying number of bright petals in each sampling pixel. On the other hand, when specific incident beams are illuminated, the output images can be reconstructed with multiple matching fields that lead to different intensities. It is essential to recognize that the bright spot in each sampling pixel of the output image achieves its peak intensity only when the matching field is the conjugate of the incident light field. This work offers a novel perspective on reconstructing holographic images through various matching fields and modulating the brightness of distinct holographic images in OAM‐multiplexing holography. Additionally, a theoretically infinite number of bright petals in each sampling pixel can also serve as information carriers in holography.

show abstract

“…FINCH requires the simplest and compact optical configuration of the above three methods with a high field of view. Due to the above reasons, FINCH became an attractive imaging system [11][12][13][14][15] and moved successfully to industry as powerful 3D fluorescence microscopy products: FINCH TM and CINCH TM [16].…”

Section: Introductionmentioning

confidence: 99%

Compact Incoherent Multidimensional Imaging Systems Using Static Diffractive Coded Apertures

Anand¹,

Ng²,

Katkus³

et al. 2023

Holography - Recent Advances and Applications

View full text Add to dashboard Cite

Incoherent holographic imaging technologies, in general, involve multiple optical components for beam splitting—combining and shaping—and in most cases, require an active optical device such as a spatial light modulator (SLM) for generating multiple phase-shifted holograms in time. The above requirements made the realization of holography-based products expensive, heavy, large, and slow. To successfully transfer the holography capabilities discussed in research articles to products, it is necessary to find methods to simplify holography architectures. In this book chapter, two important incoherent holography techniques, namely interference-based Fresnel incoherent correlation holography (FINCH) and interferenceless coded aperture correlation holography (I-COACH), have been successfully simplified in space and time using advanced manufacturing methods and nonlinear reconstruction, respectively. Both techniques have been realized in compact optical architectures using a single static diffractive optical element manufactured using lithography technologies. Randomly multiplexed diffractive lenses were manufactured using electron beam lithography for FINCH. A quasi-random lens and a mask containing a quasi-random array of pinholes were manufactured using electron beam lithography and photolithography, respectively, for I-COACH. In both cases, the compactification has been achieved without sacrificing the performances. The design, fabrication, and experiments of FINCH and I-COACH with static diffractive optical elements are presented in details.

show abstract

Large depth-of-field fluorescence microscopy based on deep learning supported by Fresnel incoherent correlation holography

Cited by 20 publications

References 37 publications

Imaging and Simulation of Ruthenium Derivative Coating Microbeads at the Opaque Electrode with Electrogenerated Chemiluminescence

Imaging and Simulation of Ruthenium Derivative Coating Microbeads at the Opaque Electrode with Electrogenerated Chemiluminescence

Superposed Laguerre‐Gaussian Beams‐Based Orbital Angular Momentum Holography

Compact Incoherent Multidimensional Imaging Systems Using Static Diffractive Coded Apertures

Contact Info

Product

Resources

About