Large doses of zinc oxide increases the activity of hydrolases in rats

Szabó, József; Hegedus, M.; Bruckner, Géza; Kósa, E.; Andrásofszky, Emese; Berta, Erzsébet

doi:10.1016/j.jnutbio.2003.09.005

Cited by 21 publications

(14 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This may be associated with the growth-promoting effect of TBZC. The lack of effect of TBZC on the activity of trypsin is contrary to the results obtained in rats where feeding large doses of ZnO resulted in increasing the activity of trypsin in pancreatic tissue as well as intestinal contents (Szabó et al, 2004). However, Hedemann et al (2006) reported that the activity of trypsin in pancreatic tissue homogenates but not small intestinal contents was elevated after feeding a high dietary concentration of ZnO.…”

Section: Resultsmentioning

confidence: 58%

Influence of tetrabasic zinc chloride and copper sulphate on growth performance and some physiological parameters in the digestive tract of weanling piglets

Zhang¹,

Guo²

2009

J. Anim. Feed Sci.

View full text Add to dashboard Cite

One hundred and sixty weanling piglets with an initial average body weight (BW) of 7.3 kg were used in a 2×2 factorial experiment to investigate interactions between tetrabasic zinc chloride (TBZC) and copper sulphate (CuSO 4 ) on growth performance. In five piglets per treatment activity of enzymes in pancreas and digesta and short-chain fatty acids (SCFA) concentration in digesta were determined. The piglets were offered the following diets for 28 days: 1. no supplementation of TBZC and CuSO 4 ; 2. supplementation of 250 mg/kg Cu from CuSO 4 ; 3. supplementation of 1500 mg/kg Zn from TBZC; 4. supplementation of 1500 mg/kg Zn from TBZC and 250 mg/kg Cu from CuSO 4 . Each treatment had five replications (pens) of eight piglets (half castrated males and half females). The results showed that average daily gain (ADG) was improved (P<0.05) by the addition of TBZC and (or) CuSO 4 . The addition of TBZC increased (P<0.05) the activity of chymotrypsin in the duodenum contents (3.145 vs 2.095, respectively). TBZC and CuSO 4 synergistically reduced (P<0.05) pH of the jejunum contents. The addition of TBZC or CuSO 4 increased (P<0.05) total SCFA concentrations, and the proportion of acetic acid and propionic acid in the colon. TBZC reduced (P<0.05) the proportions of isobutyric acid (1.470 vs 1.830, respectively) and isovaleric acid (0.171 vs 0.203, respectively), but CuSO 4 increased (P<0.01) the proportions of isobutyric acid (1.904 vs 1.396, respectively) in the colon. There was a significant interaction between TBZC and CuSO 4 for ADG (P<0.01), for trypsin activities in both pancreatic tissue homogenates (P<0.01) and duodenal contents (P<0.05) and for chymotrypsin activities in both pancreatic tissue homogenate 466 ZN AND CU -GROWTH AND DIGESTIVE TRACT OF PIGLETS (P<0.05) and jejunum contents (P<0.01), and for total SCFA concentrations in the colon. These data indicated that the growth-promotional mechanisms of TBZC and CuSO 4 might be different.

show abstract

Section: Resultsmentioning

confidence: 58%

Influence of tetrabasic zinc chloride and copper sulphate on growth performance and some physiological parameters in the digestive tract of weanling piglets

Zhang¹,

Guo²

2009

J. Anim. Feed Sci.

View full text Add to dashboard Cite

show abstract

“…Studies showed that the activities of protease, amylase and lipase were improved by high levels of Zn and were depressed with zinc deficiency (Szabo et al 2004;Jing et al 2008). Studies showed that the activities of protease, amylase and lipase were improved by high levels of Zn and were depressed with zinc deficiency (Szabo et al 2004;Jing et al 2008).…”

Section: Discussionmentioning

confidence: 99%

“…The digestion of nutrients is closely correlated with activities of digestive enzymes. Studies showed that the activities of protease, amylase and lipase were improved by high levels of Zn and were depressed with zinc deficiency (Szabo et al 2004;Jing et al 2008). In the present study, dietary zinc supplementation improved protease activities in the pancreas.…”

Section: Discussionmentioning

confidence: 99%

Changes in diarrhea, nutrients apparent digestibility, digestive enzyme activities of weaned piglets in response to chitosan‐zinc chelate

Qian

Yue

et al. 2015

Animal Science Journal

View full text Add to dashboard Cite

A total of 120 weanling barrows weighing 6.11 ± 0.20 kg were randomly allotted to four treatments with three replications (i.e. pen) of ten piglets per replicate. Pigs were received corn-soybean basal diet (control) or the same basal diet supplemented with the following sources of zinc: (i) 100 mg/kg of Zn as ZnSO4 ; (ii) 100 mg/kg of Zn as chitosan-Zn chelate (CS-Zn); and (iii) 100 mg/kg of Zn as ZnSO4 mixed with chitosan (CS + ZnSO4 ). The results showed that CS-Zn could highly improve average daily gain and average daily feed intake than those of ZnSO4 or CS+ ZnSO4 (P < 0.05). The pigs fed dietary CS-Zn had lower diarrhea incidence and higher apparent digestibility of crude protein than those of the pigs fed dietary ZnSO4 (P < 0.05). The protease activities in duodenal content of the pigs receiving CS-Zn diets was higher than that of the pigs fed dietary ZnSO4 or CS + ZnSO4 (P < 0.05). The amylase activity in duodenal content of the pigs fed dietary CS-Zn was higher than that of the pigs receiving ZnSO4 diets or basal diets (P < 0.05). These results indicated that dietary CS-Zn showed different bioactivities from ZnSO4 or CS + ZnSO4 in reducing the incidence of diarrhea, improving activities of digestive enzymes and growth performance of weaned pigs.

show abstract

“…In contrast to the present results, Carlson (2003) found that the activity of CPA, trypsin, and lipase in pancreatic tissue was lower in pigs fed 2,500 ppm of Zn compared with pigs fed 100 ppm of Zn during d 5 to 6 postweaning, whereas CPB and amylase were unaffected by Zn concentration. In rats, supplementation of the diet with high concentrations of Zn resulted in an increased enzyme activity in pancreatic tissue (Szabo et al, 2004). In chicks, however, feeding 2,000 ppm of Zn resulted in a marked decrease in the activity of amylase, lipase, trypsin, and chymotrypsin in pancreatic tissue, and the pancreas was suggested to be a target organ of Zn toxicity in chicks (Lü and Combs, 1988).…”

Section: Discussionmentioning

confidence: 99%

“…In chicks, however, feeding 2,000 ppm of Zn resulted in a marked decrease in the activity of amylase, lipase, trypsin, and chymotrypsin in pancreatic tissue, and the pancreas was suggested to be a target organ of Zn toxicity in chicks (Lü and Combs, 1988). In pigs, the tolerable level of Zn is dependent on the source of the element (Szabo et al, 2004), and toxicity of high concentrations of ZnO used for growth promotion has never been reported. The lack of effect of Cu on the enzyme activities in pancreatic homogenate is in agreement with Luo and Dove (1996).…”

Section: Discussionmentioning

confidence: 99%

Influence of dietary zinc and copper on digestive enzyme activity and intestinal morphology in weaned pigs1

Hedemann¹,

Jensen²,

Poulsen³

2006

Journal of Animal Science

147

View full text Add to dashboard Cite

The current study was conducted to investigate the effects of high dietary concentrations of Zn as zinc oxide and Cu as copper sulfate on the activity of digestive enzymes in the pancreas and the intestinal mucosa, intestinal morphology, and mucin histochemistry in pigs after weaning. Thirty-two pigs were weaned at 4 wk of age. The pigs were fed standard weaning diets supplemented with Zn (100 or 2,500 ppm) and Cu (0 or 175 ppm) in a 2 x 2 factorial arrangement of treatments for a 14-d period. In pancreatic tissue, the activity of amylase, carboxypeptidase A, chymotrypsin, trypsin, and lipase increased (P < 0.01) in pigs fed 2,500 ppm of Zn, whereas the activity of carboxypeptidase B and carboxylester hydrolase was unaffected. Copper had no effect on the activity of pancreatic enzymes. In small intestinal contents, the total activity of amylase and carboxypeptidase A was greater in pigs fed 100 ppm of Zn (P < 0.05), whereas feeding 2,500 ppm of Zn increased the chymotrypsin activity (P < 0.001). The remaining enzymes were unaffected by dietary Zn concentration. The villi were longer in the cranial small intestine (P < 0.001) in pigs fed 100 ppm of Zn than in pigs fed 2,500 ppm of Zn, but otherwise there were no clear effects of Zn and Cu supplementation on intestinal morphology. In the cranial small intestine, the activity of maltase (P < 0.001), sucrase (P < 0.001), and lactase was greater in pigs fed 100 ppm of Zn, even though there was a Zn x Cu interaction (P < 0.05) in lactase activity. In the middle and caudal small intestine, no clear differences between dietary treatments were observed. The activity of gamma-glutamyl transpeptidase in the intestinal mucosa was not affected by dietary Zn or Cu. In pigs fed 100 ppm of Zn, the activity of aminopeptidase N was greater in the caudal small intestine, but dietary Zn or Cu had no effect on aminopeptidase N in the cranial and middle small intestine. No effect of dietary Zn or Cu supplementation was found on carbohydrate histochemistry in the caudal small intestine, whereas high dietary Zn increased the area of neutral, acidic, and sulfomucins in the cecum (P < 0.01) and in the colon (P < 0.001). In summary, high dietary Zn increased the activity of several enzymes in the pancreatic tissue and increased the mucin staining area in the large intestine, whereas Cu had no clear effect on these variables. However, no definite answers were found as to how the growth promoting and diarrhea reducing effects of excess dietary Zn are exerted.

show abstract

Large doses of zinc oxide increases the activity of hydrolases in rats

Cited by 21 publications

References 14 publications

Influence of tetrabasic zinc chloride and copper sulphate on growth performance and some physiological parameters in the digestive tract of weanling piglets

Influence of tetrabasic zinc chloride and copper sulphate on growth performance and some physiological parameters in the digestive tract of weanling piglets

Changes in diarrhea, nutrients apparent digestibility, digestive enzyme activities of weaned piglets in response to chitosan‐zinc chelate

Influence of dietary zinc and copper on digestive enzyme activity and intestinal morphology in weaned pigs1

Contact Info

Product

Resources

About