Large Fecal Reservoir of Escherichia coli Sequence Type 131-H30 Subclone Strains That Are Shared Within Households and Resemble Clinical ST131-H30 Isolates

Mohamed, Muhanad; Clabots, Connie; Porter, Stephen; Bender, Tricia; Thuras, Paul; Johnson, James R.

doi:10.1093/infdis/jiz669

Cited by 13 publications

(11 citation statements)

References 39 publications

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“…Pathogenic E. coli strains are usually assigned to the B2 phylogenetic group, 43 which accounted for the main proportion (33.1%) in our research, in which ST131 E. coli was the predominant clone. ST131 ESBL-EC is commonly found in community-acquired infections, and ST131 carriers can transmit it within the families, 44 , 45 highlighting the potential health threat from ST131 carriers. Even though in this research, it was found that the detected ST131 ESBL-EC carried several virulence genes ( kpsMT II, traT, iutA, fyuA, and PAI ), the carrier did not show infection symptoms.…”

Section: Discussionmentioning

confidence: 99%

Community Fecal Carriage and Molecular Epidemiology of Extended-Spectrum β-Lactamase- and Carbapenemase-Producing Escherichia coli from Healthy Children in the Central South China

Yang

et al. 2022

IDR

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Background Fecal carriage of extended-spectrum β-lactamase-producing Escherichia coli (ESBL-EC) and carbapenemase-producing E. coli (CP-EC) is well reported among hospitalized adults and children. However, there are few studies on the carriage prevalence and ESBL-EC and CP-EC genotypes among healthy children in China. Patients and Methods Stool samples were collected from 330 students in 2021 from three randomly selected primary schools in Changsha, China. ESBL-EC and CP-EC were screened using CHROMagar TM chromogenic plates. ESBL and carbapenemase production was confirmed using the double-disc synergy test and a modified carbapenem inactivation method, respectively. Antimicrobial susceptibility was tested using the broth microdilution method. Resistance determinants, virulence factors, and phylogenetic groups were determined by PCR and sequencing. Multi-locus sequence typing (MLST) was performed (seven housekeeping genes were amplified and sequenced) on the phylogenic group B2 E. coli to detect high-risk clonal strains such as ST131 E. coli . Then, ST131 E. coli were characterized based on ST131 clades, O-type, and fimH alleles. Results In total, 118 (35.8%) ESBL-EC and 3 (0.9%) CP-EC were isolated. bla CTX-M was the most common genotype (27.1%), identified in all ESBL-EC, except one, which carried bla SHV-12 . One isolate with mcr-1 was found amongst ESBL-EC, whereas all three CP-EC carried bla NDM-1 . The predominant sequence type (ST) clones in group B2 were ST131 and ST1193. The prevalence of ST131 E. coli was 9.9%, displaying serotypes O16 and O25b, fimH alleles 30, 41, and 89, and ST131 clades A and C1-M27. Conclusion In this study, high carriage rate of ESBL-EC was found among healthy children, and the dominant ESBL was CTX-M-14. In addition, high-risk clones (ST131 and ST1193) were also detected. This emphasizes the importance of monitoring ESBL-EC in community settings.

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Section: Discussionmentioning

confidence: 99%

Community Fecal Carriage and Molecular Epidemiology of Extended-Spectrum β-Lactamase- and Carbapenemase-Producing Escherichia coli from Healthy Children in the Central South China

Yang

et al. 2022

IDR

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“…The use of contemporary isolates in our sampling increased our ability of finding clonal isolates that overlap between hosts, households and sublocations. Previous work using whole genomes either found no overlap or isolates that were separated by more than ten core SNPs, which does not provide strong evidence for a recent sharing event (28,29). While challenging in practice, we have demonstrated the importance of large-scale structured sampling to understand strain sharing at the population level.…”

Section: Discussionmentioning

confidence: 59%

Landscape genomics of Escherichia coli in livestock-keeping households across a rapidly developing urban city

Muloi¹,

Wee

McClean

et al. 2021

Preprint

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The keeping of livestock has been posited as a risk factor for the emergence of zoonoses and the spread of antimicrobial resistance. However, quantitative evidence regarding the major sources of pathogenic and drug-resistant bacteria and transmission routes between hosts remains lacking. In the largest epidemiological study of this nature to date, we sampled Escherichia coli from humans, livestock, food, wildlife and the environment of 99 households across Nairobi, Kenya to gain a deeper understanding of sharing of bacteria among hosts and potential reservoirs. By analysing whole genome sequencing data from 1,338 E. coli isolates, we reconstruct sharing patterns for the sampled E. coli and its antimicrobial resistance determinants. We find that the diversity and sharing patterns of E. coli is heavily structured by household, which is the primary epidemiological interface for bacterial strain sharing. Strain sharing within households was strongly shaped by host type. We also find evidence for inter-household and inter-host sharing, and importantly, between humans and animals, although this occurs much less frequently. We find similar strain sharing patterns for the E. coli accessory genome, suggesting that it is shaped by recent evolutionary history and is strongly associated with the core genome. Resistome similarity, however, were quite differently distributed across host and household, consistent with their being driven by shared exposure to antimicrobials. Our results indicate that there is potential for the exchange of bacteria between humans, livestock and wildlife in the same household in a tropical urban setting, with wider mixing occurring over a period of months or years, but this does not drive the distribution of antimicrobial resistance.

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“…Morphologically consistent, indole-positive, citrate-negative colonies were regarded as E. coli . Up to 10 E. coli colonies per plate (as available) underwent random amplified polymorphic DNA (RAPD) analysis using primer 1254 according to an established procedure adapted from Berg et al and optimized by the authors [ 22 , 23 ]. In validation studies, when applied to replicate colonies from a given fecal sample, this approach yielded 85%–90% concordance with Xbal pulsed-field gel electrophoresis (PFGE) (unpublished, J.R.J.).…”

Section: Methodsmentioning

confidence: 99%

Bacterial “Virulence” Traits and Host Demographics Predict Escherichia coli Colonization Behaviors Within Households

Fox

Clabots

Porter

et al. 2020

Open Forum Infectious Diseases

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Background Although intestinal colonization precedes most extraintestinal Escherichia coli infections, colonization-promoting factors are incompletely understood. We compared within-household E. coli colonization patterns with host and bacterial traits. Methods Twenty-two veterans with a clinical E. coli isolate and their 46 human and animal household members underwent longitudinal fecal sampling. Distinct E. coli strains were characterized for phylogenetic background, virulence genes, antibiotic resistance, and colonization behaviors. Host and bacterial traits were assessed statistically as predictors of colonization behaviors. Results Among the 139 unique-by-household fecal E. coli strains, univariable predictors of colonization behavior included (i) host demographics, (ii) matching the index clinical isolate, and (iii) bacterial characteristics (2 phylogroups, 5 clonal lineages, 18 virulence genes, and molecular extraintestinal pathogenic E. coli status). Multivariable predictors of colonization behavior included: veteran host, spouse host, matching the index clinical isolate, phylogroup F, ST73, hlyD (alpha hemolysin), hlyF (variant hemolysin), H7 fliC (flagellar variant), vat (vacuolating toxin), and iha (adhesin-siderophore). Conclusions Host demographics, multiple bacterial "virulence" traits, and matching the index clinical isolate predicted E. coli fecal colonization behaviors. Thus, certain bacterial characteristics may promote both colonization and pathogenicity. Future interventions directed toward such traits might prevent E. coli infections both directly and by disrupting antecedent colonization.

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Large Fecal Reservoir of Escherichia coli Sequence Type 131-H30 Subclone Strains That Are Shared Within Households and Resemble Clinical ST131-H30 Isolates

Cited by 13 publications

References 39 publications

Community Fecal Carriage and Molecular Epidemiology of Extended-Spectrum β-Lactamase- and Carbapenemase-Producing Escherichia coli from Healthy Children in the Central South China

Community Fecal Carriage and Molecular Epidemiology of Extended-Spectrum β-Lactamase- and Carbapenemase-Producing Escherichia coli from Healthy Children in the Central South China

Landscape genomics of Escherichia coli in livestock-keeping households across a rapidly developing urban city

Bacterial “Virulence” Traits and Host Demographics Predict Escherichia coli Colonization Behaviors Within Households

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