Large generation of megakaryocytes from serum-free expanded human CD34+ cells

Chen, Te-Wei; Yao, Chao‐Ling; Chu, I‐Ming; Chuang, Tzu-Lin; Hsieh, Tzu‐Bou; Hwang, Shiaw-Min

doi:10.1016/j.bbrc.2008.11.019

Cited by 26 publications

(18 citation statements)

References 20 publications

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“…Inclusion of the PDGFR antagonist MK1 counteracts these effects thereby promoting megakaryocyte differentiation. The observation that PDGF-CC is expressed by CD34 + cells may explain why previous efforts supplementing cultures with exogenous PDGF failed to identify a role for PDGF in megakaryopoiesis (27). The observation that the action of MK1 happens at the expense of GM differentiation, but not at the expense of erythrocyte differentiation, further supports the notion that MK1 acts at the CMP stage.…”

Section: Resultssupporting

confidence: 66%

An image-based screen identifies a small molecule regulator of megakaryopoiesis

Boitano

Lichtervelde

Snead

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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Molecules that control the lineage commitment of hematopoietic stem cells (HSCs) may allow the expansion of enriched progenitor populations for both research and therapeutic uses. In an effort to better understand and control the differentiation of HSCs to megakaryocytes, we carried out an image-based screen of a library of 50,000 heterocycles using primary human CD34 + cells. A class of naphthyridinone derivatives was identified that induces the differentiation of common myeloid progenitors (CMP) to megakaryocytes. Kinase profiling and subsequent functional assays revealed that these compounds act through inhibition of platelet-derived growth factor receptor (PDGFR) signaling in CMPs. Such molecules may ultimately have clinical utility in the treatment of thrombocytopenia.

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Section: Resultssupporting

confidence: 66%

An image-based screen identifies a small molecule regulator of megakaryopoiesis

Boitano

Lichtervelde

Snead

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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“…The platelets produced were partially preactivated, which is consistent with other studies assessing the properties of culture-derived platelets. 72,73 However, exposure to PMA and thrombin increased activationdependent surface marker expression on all CD41…”

Section: Discussionmentioning

confidence: 99%

Three-StageEx VivoExpansion of High-Ploidy Megakaryocytic Cells: Toward Large-Scale Platelet Production

Panuganti

Schlinker

Lindholm

et al. 2013

Tissue Engineering Part A

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“…Regardless of the abundant advantages of using UCB for transplantation compared to other cell sources, the low number of HSPCs contained in a single UCB unit, and the consequently delayed platelet recovery, has limited successful transplantation (Laughlin 2001, Koh et al 2007). Platelet recovery in cord blood (CB) transplantation takes a longer period compared to that in bone marrow (BM) or mobilized peripheral blood (MPB) cell transplantation (Chen et al 2009), as the average of number of days for platelet engraftment in UCB and MPB is nearly 70 and 20 days, respectively (Ignatz et al 2007). Th us, ex vivo expansion of megakaryocytes (Mk) derived from UCB along with HSCs might accelerate Mk diff erentiation and platelet recovery after transplantation.…”

Section: Introductionmentioning

confidence: 98%