Large genomic differences between Moraxella bovoculi isolates acquired from the eyes of cattle with infectious bovine keratoconjunctivitis versus the deep nasopharynx of asymptomatic cattle

Abstract

Moraxella bovoculi is a recently described bacterium that is associated with infectious bovine keratoconjunctivitis (IBK) or “pinkeye” in cattle. In this study, closed circularized genomes were generated for seven M. bovoculi isolates: three that originated from the eyes of clinical IBK bovine cases and four from the deep nasopharynx of asymptomatic cattle. Isolates that originated from the eyes of IBK cases profoundly differed from those that originated from the nasopharynx of asymptomatic cattle in genome structure, gene content and polymorphism diversity and consequently placed into two distinct phylogenetic groups. These results suggest that there are genetically distinct strains of M. bovoculi that may not associate with IBK.Electronic supplementary materialThe online version of this article (doi:10.1186/s13567-016-0316-2) contains supplementary material, which is available to authorized users.

Infectious keratoconjunctivitis in semi-domesticated Eurasian tundra reindeer (Rangifer tarandus tarandus): microbiological study of clinically affected and unaffected animals with special reference to cervid herpesvirus 2

“…Dickey et al [ 50 ] showed that there are large genomic differences between Moraxella bovoculi isolates from IBK affected cattle and asymptomatic animals. These results open the possibility to the presence of different strains of Moraxella bovoculi with different pathogenic potential.…”

Antimicrobial usage and resistance in beef production

“…isolated from cattle and sheep, Maboni et al [119] found that 40% of isolates were penicillin-resistant and 20% were tetracycline-resistant, but most were susceptible to other antimicrobials. Dickey et al [120] published the genome sequence for an AMR isolate of Moraxella bovoculi , Mb58069. It was found to be resistant to florfenicol, oxytetracycline, sulfonamides, and displayed intermediate resistance to macrolides.…”

“…It was found to be resistant to florfenicol, oxytetracycline, sulfonamides, and displayed intermediate resistance to macrolides. Ten AMR determinants were co-located on a >27 kb genomic island [120]. The biofilm-forming capabilities of Moraxella bovis may also enhance antimicrobial resistance.…”

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry identification of Moraxella bovoculi and Moraxella bovis isolates from cattle

“…These strains may be similar to the genetically atypical, non-IBK strains of M. bovoculi described previously. 7 The M. bovis isolates that matched to database isolate M. bovis 2017003602-1 in the UNLVDC library were also those that demonstrated interspecies recombination at the ribosomal loci ( Supplementary Fig. 1), indicating the potential for MALDI-TOF MS to detect these recombinants.…”

“…7,16 All 268 isolates were subjected to whole genome paired-end sequencing (MiSeq instrument, Illumina, San Diego, CA) at the U.S. Meat Animal Research Center with either MiSeq reagent kit v.2 (2 × 250 bp, 500 cycles) or v.3 (2 × 300 bp, 600 cycles). Resulting sequences were mapped to a M. bovoculi reference genome, strain CP011374, 7 in Geneious v.10. 8 The mapped sequences of each isolate were visually checked for evidence of "mixed" isolate contamination, which was not detected, indicating that the sequences represented a single haploid isolate genome.…”