Large-scale and high-confidence proteomic analysis of human seminal plasma

Pilch, Bartosz; Mann, Matthias

doi:10.1186/gb-2006-7-5-r40

Cited by 326 publications

(135 citation statements)

References 35 publications

Supporting

Mentioning

129

Contrasting

Unclassified

Order By: Relevance

“…These are secreted from different sources including seminal vesicle, prostate, vas deference, and epididymus. The present study shows the 2-D map of human seminal plasma describing 917 spots, which is in agreement with previously reported data [1,[25][26]. The purpose of performing a 2-D experiment was to identify the proteins, which were overexpressed in prostate carcinoma.…”

Section: Discussionsupporting

confidence: 78%

See 1 more Smart Citation

Proteomic approach for purification of seminal plasma proteins involved in tumor proliferation

Hassan

Kumar

Kashav

et al. 2007

J of Separation Science

View full text Add to dashboard Cite

Human seminal plasma contains a large array of proteins required for the normal physiology and metabolism of spermatozoa. These are mainly secreted from prostate epithelium, testes, and seminal vesicles. Fortunately, many of these are found to be present at elevated concentration in seminal plasma and act as a biomarker of different carcinomas as their levels are also enhanced in serum and are found to be involved in tumor progression and metastasis apart from fertility. The proteins which were overexpressed in the seminal plasma of prostate carcinoma patients were identified by 2-DE and MALDI-TOF/MS. We have designed a strategy to purify these four proteins prostate specific antigen (PSA), prostatic acid phosphatase (PAP), Zinc alpha2-glycoprotein (ZAG), and progastricsin (PG), together in homogeneity by using simple chromatographic techniques. Acidic and basic fractions of human seminal plasma were separated by ion exchange chromatography and further purified by gel permeation chromatography. Our results form a new and valuable resource for those attempting structure-based drug designing for prostate and other cancers where the amount of proteins is required in plenty and in native form.

show abstract

Section: Discussionsupporting

confidence: 78%

“…The characterization of seminal plasma protein composition provides their potential roles in fertilization. When combined with quantitative proteomics methodologies, it may be useful for studies of fertilization, male fertility, and prostatic and testicular cancers [1].…”

Section: Introductionmentioning

confidence: 99%

Proteomic approach for purification of seminal plasma proteins involved in tumor proliferation

Hassan

Kumar

Kashav

et al. 2007

J of Separation Science

View full text Add to dashboard Cite

show abstract

“…Based on recent proteomic analyses, this activity is likely fucosyltransferase III (65). The activity of this fucosyltransferase declines significantly after anti-androgen therapy (66), suggesting that its expression is regulated by sexlinked hormones.…”

Section: Discussionmentioning

confidence: 99%

Expression of Bisecting Type and Lewisx/Lewisy Terminated N-Glycans on Human Sperm

Pang

Tissot

Drobnis

et al. 2007

Journal of Biological Chemistry

View full text Add to dashboard Cite

Human sperm lack major histocompatibility class I molecules, making them susceptible to lysis by natural killer (NK) cells. Major histocompatibility class I negative tumor cells block NK cell lysis by expressing sufficient amounts of bisecting type N-glycans on their surfaces. Therefore, sperm could employ the same strategy to evade NK cell lysis. The total N-glycans derived from sperm were sequenced using ultrasensitive mass spectrometric and conventional approaches. Three major classes of N-glycans were detected, (i) high mannose, (ii) biantennary bisecting type, and (iii) biantennary, triantennary, and tetraantennary oligosaccharides terminated with Lewis x and Lewis y sequences. Immunostaining of normal sperm showed that glycoproteins bearing Lewis y sequences are localized to the acrosome and not the plasma membrane. In contrast, defective sperm showed distinct surface labeling with antiLewis y antibody. The substantial expression of high mannose and complex type N-glycans terminated with Lewis x and Lewis y sequences suggests that sperm glycoproteins are highly decorated with ligands for DC-SIGN. Based on previous studies, the addition of such carbohydrate signals should inhibit antigenspecific responses directed against sperm glycoproteins in both the male and female reproductive systems. Thus, the major N-glycans of human sperm are associated with the inhibition of both innate and adaptive immune responses. These results provide more support for the eutherian fetoembryonic defense system hypothesis that links the expression of carbohydrate functional groups to the protection of gametes and the developing human in utero. This study also highlights the usefulness of glycomic profiling for revealing potential physiological functions of glycans expressed in specific cell types.Studies performed over the past four decades indicate that conditions are not optimal for human sperm in the female reproductive system (1). Women release neutrophils, monocytes, and lymphocytes from the specialized mucosal surface of their cervix during the leukocyte reaction after coitus (2, 3). This sequestration occurs specifically in response to contact with sperm but not seminal plasma (2, 3). Increased immunological awareness related to histocompatibility and its implications for reproduction in eutherians initially raised concerns about how foreign human sperm are tolerated in the female reproductive system (4). Early studies suggested that human sperm express MHC 3 class I molecules that define self, suggesting the possibility that histocompatibility based responses must be suppressed in the female reproductive tract (5). However, more recent studies confirm that sperm precursors down-modulate their MHC class I molecules, yielding mature sperm that are MHC class I negative cells (5). This absence enables these gametes to evade histocompatibility based responses mediated by MHC class I-restricted cytotoxic T lymphocytes (6).This lack of MHC class I molecules does not make sperm completely invulnerable to cell mediated responses...

show abstract

“…These tissues produce, modify, and store secreted proteins that are then transferred to females during mating, along with sperm. For some species there are comprehensive data on SFPs from large-scale transcriptomic and/or proteomic analyses (e.g., Collins et al 2006; Pilch and Mann 2006; Dottorini et al 2007; Findlay et al 2008, 2009; Sirot et al 2008, 2011; Walters and Harrsion 2008, 2010; Baer et al 2009; Dean et al 2009; Ramm et al 2009; Rogers et al 2009; Claw 2013). In others, only a partial complement of SFPs has been identified so far (e.g., Andrés et al 2006, 2008; Davies and Chapman 2006; South et al 2011; Simmons et al 2013).…”

Section: The Battlegroundmentioning

confidence: 99%

Sexual Conflict and Seminal Fluid Proteins: A Dynamic Landscape of Sexual Interactions

Sirot

Wong

Chapman

et al. 2014

Cold Spring Harb Perspect Biol

156

221

View full text Add to dashboard Cite

Sexual reproduction requires coordinated contributions from both sexes to proceed efficiently. However, the reproductive strategies that the sexes adopt often have the potential to give rise to sexual conflict because they can result in divergent, sex-specific costs and benefits. These conflicts can occur at many levels, from molecular to behavioral. Here, we consider sexual conflict mediated through the actions of seminal fluid proteins. These proteins provide many excellent examples in which to trace the operation of sexual conflict from molecules through to behavior. Seminal fluid proteins are made by males and provided to females during mating. As agents that can modulate egg production at several steps, as well as reproductive behavior, sperm “management,” and female feeding, activity, and longevity, the actions of seminal proteins are prime targets for sexual conflict. We review these actions in the context of sexual conflict. We discuss genomic signatures in seminal protein (and related) genes that are consistent with current or previous sexual conflict. Finally, we note promising areas for future study and highlight real-world practical situations that will benefit from understanding the nature of sexual conflicts mediated by seminal proteins.

show abstract

Large-scale and high-confidence proteomic analysis of human seminal plasma

Cited by 326 publications

References 35 publications

Proteomic approach for purification of seminal plasma proteins involved in tumor proliferation

Proteomic approach for purification of seminal plasma proteins involved in tumor proliferation

Expression of Bisecting Type and Lewisx/Lewisy Terminated N-Glycans on Human Sperm

Sexual Conflict and Seminal Fluid Proteins: A Dynamic Landscape of Sexual Interactions

Contact Info

Product

Resources

About