Large-Scale Arrays of Bowtie Nanoaperture Antennas for Nanoscale Dynamics in Living Cell Membranes

Flauraud, Valentin; Zanten, Thomas S. van; Mivelle, Mathieu; Manzo, Carlo; Parajo, M.F. Garcia; Brugger, Jürgen

doi:10.1021/acs.nanolett.5b01335

Cited by 46 publications

(70 citation statements)

References 41 publications

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“…[23][24][25][26] Moreover, when combined with FCS, single molecule detection at ultrahigh sample concentrations with microsecond time resolution can be obtained, both in solution and living cell membranes. [27][28][29][30][31] However, in most antenna designs the region of maximum field localization and enhancement (i.e., hotspot) is buried into the nanostructure, and thus difficult to access. Recently, we overcame this drawback by fabricating in-plane dimer antenna arrays where the gap region is located at the sample surface.…”

Section: Abstract: Optical Nano-antennas; Fluorescence Correlation Spmentioning

confidence: 99%

Transient Nanoscopic Phase Separation in Biological Lipid Membranes Resolved by Planar Plasmonic Antennas

et al. 2017

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Nanoscale membrane assemblies of sphingolipids, cholesterol, and certain proteins, also known as lipid rafts, play a crucial role in facilitating a broad range of important cell functions. Whereas on living cell membranes lipid rafts have been postulated to have nanoscopic dimensions and to be highly transient, the existence of a similar type of dynamic nanodomains in multicomponent lipid bilayers has been questioned. Here, we perform fluorescence correlation spectroscopy on planar plasmonic antenna arrays with different nanogap sizes to assess the dynamic nanoscale organization of mimetic biological membranes. Our approach takes advantage of the highly enhanced and confined excitation light provided by the nanoantennas together with their outstanding planarity to investigate membrane regions as small as 10 nm in size with microsecond time resolution. Our diffusion data are consistent with the coexistence of transient nanoscopic domains in both the liquid-ordered and the liquid-disordered microscopic phases of multicomponent lipid bilayers. These nanodomains have characteristic residence times between 30 and 150 μs and sizes around 10 nm, as inferred from the diffusion data. Thus, although microscale phase separation occurs on mimetic membranes, nanoscopic domains also coexist, suggesting that these transient assemblies might be similar to those occurring in living cells, which in the absence of raft-stabilizing proteins are poised to be short-lived. Importantly, our work underscores the high potential of photonic nanoantennas to interrogate the nanoscale heterogeneity of native biological membranes with ultrahigh spatiotemporal resolution. KEYWORDS: optical nanoantennas, fluorescence correlation spectroscopy, FCS diffusion laws, biological membranes, lipid rafts T he spatiotemporal lateral organization and the biological function of the eukaryotic plasma membrane are intricately interlaced at the nanoscale. It is well accepted that the landscape of the cell membrane is highly heterogeneous and shaped by a variety of lipids and proteins that differ in their physicochemical properties. In the plane of the membrane, lateral heterogeneities resulting from the formation of specialized regions enriched in sphingolipids, cholesterol, and specific proteins are commonly known as lipid rafts.1−4 These lipid assemblies are thought to constitute a tightly packed, short-range, liquid-ordered (Lo) phase coexisting with a more liquid-disordered (Ld) phase within the surrounding fluid membrane.5−7 While the existence of phase separation in the plasma membrane has been debated for many years, a large number of recent experimental data convincingly demonstrates that lipid rafts in living cell membranes have nanoscopic dimensions and are highly dynamic.8−13 Importantly, lipid rafts play a crucial role in many cellular processes that include signal transduction, protein and lipid sorting, and immune response among others. 2,5,10,14,15 Understanding the formation mechanism and properties (e.g., size, composition) of lipid...

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Section: Abstract: Optical Nano-antennas; Fluorescence Correlation Spmentioning

confidence: 99%

Transient Nanoscopic Phase Separation in Biological Lipid Membranes Resolved by Planar Plasmonic Antennas

et al. 2017

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“…Not only should nanogaps with sub 20 nm dimensions be reproducibly fabricated, but also the gap region (plasmonic hotspot) must remain accessible to probe the target molecules. Despite impressive recent progress using electron beam, 22 focused ion beam 23 or stencil lithographies, 24,25,26 or alternatively with bottom-up self-assembly techniques, 6,7,9,13,16,[27][28][29][30] the challenges of reliable narrow gap fabrication and hotspot accessibility remain major hurdles limiting the impact and performance of optical nanoantennas. For instance, when aiming for the fabrication of aperture antennas, electron beam lithography (EBL) using a positive-tone resist requires metal dry etching, which produces high line-edge roughness that are not suited for the definition of reliable and high aspect ratio nanogaps.…”

mentioning

confidence: 99%

Effect of Thermal Fluctuations on the Radiative Rate in Core/Shell Quantum Dots

Balan

Eshet²,

Olshansky

et al. 2017

Nano Lett.

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The effect of lattice fluctuations and electronic excitations on the radiative rate is demonstrated in CdSe/CdS core/shell spherical quantum dots (QDs). Using a combination of time-resolved photoluminescence spectroscopy and atomistic simulations, we show that lattice fluctuations can change the radiative rate over the temperature range from 78 to 300 K. We posit that the presence of the core/shell interface plays a significant role in dictating this behavior. We show that the other major factor that underpins the change in radiative rate with temperature is the presence of higher energy states corresponding to electron excitation into the shell. These effects should be present in other core/shell samples and should also affect other excited state rates, such as the rate of Auger recombination or the rate of charge transfer.

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“…As a result, the power throughput was improved ~3 orders of magnitude, the signal intensity of single molecule was enhanced ~6 folds [4]. With the bowtie nanoaperture probe, the optical confinement of 20~80 nm was obtained [8,9], which opened the possibility to image the molecules smaller than ~50 nm.…”

Section: Reviewmentioning

confidence: 99%

Antennas based near-field scanning optical microscopy (NSOM) for protein imaging beyond diffraction limit

Zhang¹,

Yong²

2018

Ann Biotechnol

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In recent years, super resolution microscopy has been rapidly applied in biological study. Near-field Scanning Optical Microscopy (NSOM) is one of the most powerful optical super resolution microscopy that can be used for protein nanoimaging in living cells. Moreover, optical antennas have also been adapted to NSOM and greatly improved the performance of NSOM. In this mini review, the application of different optical antennas for NSOM has been described, and the use of NSOM for protein nanoimaging has also been highlighted.

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Large-Scale Arrays of Bowtie Nanoaperture Antennas for Nanoscale Dynamics in Living Cell Membranes

Cited by 46 publications

References 41 publications

Transient Nanoscopic Phase Separation in Biological Lipid Membranes Resolved by Planar Plasmonic Antennas

Transient Nanoscopic Phase Separation in Biological Lipid Membranes Resolved by Planar Plasmonic Antennas

Effect of Thermal Fluctuations on the Radiative Rate in Core/Shell Quantum Dots

Antennas based near-field scanning optical microscopy (NSOM) for protein imaging beyond diffraction limit

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