Large scale bioaugmentation of soil contaminated with petroleum hydrocarbons using a mixed microbial consortium

Poi, Gregory; Aburto‐Medina, Arturo; Mok, Puah Chum; Ball, Andrew S.; Shahsavari, Esmaeil

doi:10.1016/j.ecoleng.2017.01.048

Cited by 57 publications

(25 citation statements)

References 75 publications

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“…In bioaugmentation, the use of both consortia and pure cultures has been tested; bacteria are usually considered more effective than fungi in the biodegradation of hydrocarbons . Some of the most active bacterial hydrocarbon degraders are the Pseudomonas , Rhodococcus , Acinetobacter and Alcaligenes genera …”

Section: Introductionmentioning

confidence: 99%

“…21 Some of the most active bacterial hydrocarbon degraders are the Pseudomonas, Rhodococcus, Acinetobacter and Alcaligenes genera. 11,12,[22][23][24] Bioaugmentation can also be coupled with biostimulation to further stimulate the introduced biomass. In fact, microorganisms may consume soil mineral nutrients too rapidly for the metabolism of the organic material, and the introduction of microorganisms could therefore require the addition of mineral nutrients to be effective.…”

Section: Introductionmentioning

confidence: 99%

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Microcosm evaluation of bioaugmentation and biostimulation efficacy on diesel‐contaminated soil

Bosco

Casale

Mazzarino

et al. 2019

J of Chemical Tech & Biotech

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BACKGROUND Diesel oil broad utilization has led to its dispersion in the environment. In this work, biostimulation and biostimulation coupled with autochthonous bioaugmentation were investigated for the bioremediation of soil artificially contaminated with diesel oil. RESULTS Soil microcosms were prepared and monitored. The most effective strategy and the influence of glucose addition, both in soil or during the biomass ‘enrichment’ phase, were evaluated by means of respirometric analyses, microbial counts and residual contaminant concentration measurements. The respirometric activity was always higher in all microcosms contaminated with diesel oil than in the biotic controls. The presence of the contaminant did not inhibit the microbial activity. The microbial counts performed on diesel oil medium showed that the contaminant was prevalently biodegraded by the indigenous bacterial species. In terms of diesel oil degradation, similar results were obtained in biostimulated and bioaugmented microcosms; a degradation percentage in the range 65–70% after 34 days was achieved. The addition of glucose in soil, though favoring the growth of microorganisms, did not improve the degradation of contaminant. CONCLUSION In this work, biostimulation, with no addition of a primary substrate, was the simplest and most cost‐effective strategy for the biodegradation of diesel oil. © 2019 Society of Chemical Industry

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Microcosm evaluation of bioaugmentation and biostimulation efficacy on diesel‐contaminated soil

Bosco

Casale

Mazzarino

et al. 2019

J of Chemical Tech & Biotech

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“…For its removal, in situ bioremediation is considered an environmentally friendly and cost-effective solution, due to the metabolic capability of microorganisms to degrade the pollutants. So far, bioremediation is performed by means of • biostimulation: The addition of macro-and/or micronutrients to enhance indigenous biomass growth and pollutant degradation [1,2]; • bioaugmentation: The addition of enriched microbial cultures (autochthonous or allochthonous degraders) to the soil [3]; • combined biostimulation and bioaugmentation [4,5].…”

Section: Introductionmentioning

confidence: 99%

“…Several studies were carried out in different operative conditions, adopting one of the aforesaid strategies, and aimed to optimize hydrocarbon removal [3,[6][7][8][9]. It is widely known that biostimulation is an easier process to carry out [2,5,10,11].…”

Section: Introductionmentioning

confidence: 99%

Removal of Diesel Oil in Soil Microcosms and Implication for Geophysical Monitoring

Bosco

Casale

Chiampo

et al. 2019

Water

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Bioremediation of soils polluted with diesel oil is one of the methods already applied on a large scale. However, several questions remain open surrounding the operative conditions and biological strategies to be adopted to optimize the removal efficiency. This study aimed to investigate the environmental factors that influence geophysical properties in soil polluted with diesel oils, in particular, during the biodegradation of this contaminant by an indigenous microbial population. With this aim, aerobic degradation was performed in soil column microcosms with a high concentration of diesel oil (75 g kg −1 of soil); the dielectric permittivity and electrical conductivity were measured. In one of the microcosms, the addition of glucose was also tested. Biostimulation was performed with a Mineral Salt Medium for Bacteria. The sensitivity of the dielectric permittivity versus temperature was analyzed. A theoretical approach was adopted to estimate the changes in the bulk dielectric permittivity of a mixture of sandy soil-water-oil-gas, according to the variations in the oil content. The sensitivity of the dielectric permittivity to the temperature effects was analyzed. The results show that (1) biostimulation can give good removal efficiency; (2) the addition of glucose as a primary carbon source does not improve the diesel oil removal; (3) a limited amount of diesel oil was removed by adsorption and volatilization effects; and (4) the diesel oil efficiency removal was in the order of 70% after 200 days, with different removal percentages for oil components; the best results were obtained for molecules with a low retention time. This study is preparatory to the adoption of geophysical methods to monitor the biological process on a larger scale. Altogether, these results will be useful to apply the process on a larger scale, where geophysical methods will be adopted for monitoring.

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“…For instance, Poi et al employed an adapted indigenous consortium consisting of 22 strains: mainly Bacillus, Pseudomonas and Acinetobacter spp. in a large scale bioaugmentation system for clean-up of soil contaminated with petroleum hydrocarbons; they concluded that this system constitutes a proper method for the treatment of full-scale petroleum polluted soil (Poi et al, 2017). It is entirely clear that the main upside of using a consortium, instead of a single microorganism, is its synergistic effect, because one species degrades toxic metabolites, which may hinder the growth of other strains and, in turn, this collective system promotes the bioremediation process (Ghazali et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Bioremediation of actual soil samples with high levels of crude oil using a bacterial consortium isolated from two polluted sites: investigation of the survival of the bacteria

2018

Global NEST Journal

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In this study, actual soil samples, taken from Kharg Island, Iran, with high levels of total petroleum hydrocarbons (TPHs) were bioremediated by a bacterial consortium. The strains were: Pseudomonas sp., Stenotrophomonas sp., Achromobacter sp., Brevibacillus sp. and Staphylococcus sp., which were isolated from two highly polluted sites: Kharg Island and Kermanshah Oil Refinery. The bacteria were identified and confirmed by biochemical tests and the PCR assay. In order to isolate the bacteria, the enrichment method, in which crude oil (2%) was utilized as the sole source of carbon and energy, was used. The results showed that the adapted bacterial consortium could degrade nearly 60% of the pollution. Also, it was found that after 100 days of bioremediation only Brevibacillus sp. could survive in the environment.

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Large scale bioaugmentation of soil contaminated with petroleum hydrocarbons using a mixed microbial consortium

Cited by 57 publications

References 75 publications

Microcosm evaluation of bioaugmentation and biostimulation efficacy on diesel‐contaminated soil

Microcosm evaluation of bioaugmentation and biostimulation efficacy on diesel‐contaminated soil

Removal of Diesel Oil in Soil Microcosms and Implication for Geophysical Monitoring

Bioremediation of actual soil samples with high levels of crude oil using a bacterial consortium isolated from two polluted sites: investigation of the survival of the bacteria

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