2012
DOI: 10.1080/10826068.2011.627971
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LARGE SCALE BIOSYNTHESIS OF GANGLIOSIDE ANALOGUES BY RERF-LC-AI CELLS CULTURED IN HYPERFlask

Abstract: The efficient production of ganglioside analogues was accomplished using RERF-LC-AI cells cultured in HYPERFlask (High Yield PERformance Flask). Eight kinds of ganglioside analogues (GM3, GM2, sialylparagloboside, GD3, di-sialylated lacto-N-tetraose, and another three kinds of analogues with intricate structures) were synthesized by the saccharide primer method using lung squamous-cell carcinoma line RERF-LC-AI and 12-azidododecyl β-lactoside primer. The yield for each analogue obtained using HYPERFlask was hi… Show more

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Cited by 3 publications
(2 citation statements)
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“…For the biotechnological production of ganglioside head groups, lactose can be internalized in E. coli as a precursor to be used as acceptor for glycosyltransferases [ 410 415 ]. An application of the ganglioside biosynthetic machinery is the preparative production of neoglycolipids with ganglioside head groups using a lung squamous-cell carcinoma line (RERF-LC-A) and 12-azidododecyl β -lactoside as a suitable primer [ 416 ].…”
Section: Toolsmentioning
confidence: 99%
“…For the biotechnological production of ganglioside head groups, lactose can be internalized in E. coli as a precursor to be used as acceptor for glycosyltransferases [ 410 415 ]. An application of the ganglioside biosynthetic machinery is the preparative production of neoglycolipids with ganglioside head groups using a lung squamous-cell carcinoma line (RERF-LC-A) and 12-azidododecyl β -lactoside as a suitable primer [ 416 ].…”
Section: Toolsmentioning
confidence: 99%
“…TOF, FAB Synthesis of unique GSL from Aplysinella rhax (Fujita et al, 2011c) Ganglioside analogues TOF Large-scale production by lung squamous-cell carcinoma RERF-LC-AI cells cultured in HyperFlask (Shimura et al, 2012b) Gangliosides with Neu5Ac-2 3Gal and Neu5Ac-2 6Gal…”
Section: Glycosidesmentioning
confidence: 99%