Large-scale characterization of promoters from grapevine (Vitis spp.) using quantitative anthocyanin and GUS assay systems

Li, Zhijian T.; Kim, Kyung‐Hee; Jasinski, Jonathon R.; Creech, Matthew; Gray, D. J.

doi:10.1016/j.plantsci.2012.08.009

Cited by 27 publications

(19 citation statements)

References 50 publications

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“…Transformed plants, once recovered, will expectedly have no growth-interfering anthocyanin overexpression and accumulation in vegetative tissues. The nondestructive anthocyanin reporter system should offer a useful tool for high-throughput evaluation of plant promoters (Li et al 2012 ).…”

Section: Grapevinementioning

confidence: 99%

Promoters for Transgenic Horticultural Plants

Smirnova

Tishchenko²,

Ermakov

et al. 2014

Abiotic Stress Biology in Horticultural Plants

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Gene engineering provides an opportunity to obtain plants with either silenced or overexpressed target genes. This approach is frequently used to study various aspects of the biochemistry, physiology, and stress tolerance of horticultural plants. Selection of appropriate promoters to govern transcription of a transgenic construct is an important step in the development of effi cient genetic models. Here we present a brief overview of available literature data on the promoters investigated with the transgenic horticultural plants and some valuable information resources in this fi led.

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Section: Grapevinementioning

confidence: 99%

Promoters for Transgenic Horticultural Plants

Smirnova

Tishchenko²,

Ermakov

et al. 2014

Abiotic Stress Biology in Horticultural Plants

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“…In grapevines, this technology has been recently applied to analyze the function of several genes such as VviAdh [75], VviPIP2 [76], VviCCD1 [77], and VviWRKY33 [78]. Likewise, this method has been proved to be very useful for transgenic complementation [79][80][81], technical studies [82][83][84][85][86][87], promoter analysis [88][89][90][91], subcellular localization analysis [92], and protein production [69,93] in grapevine. Nonetheless, the genetic background and plant growth conditions are key factors in performing successful Agrobacterium-mediated transformation.…”

Section: Indirect Gene Transfer Methods: Agrobacterium-mediated Transmentioning

confidence: 99%

“…For instance, the compatibility of A. tumefaciens strains with the plant species represents an important variable to be considered in this kind of assay. Thus, the strains most efficiently used for gene transfer into grapevine are probably C58C1 (pCH32) [69,78,86] and EHA105 [75,77,82,83,85,88,90], which contain extra copies of vir genes that make them hypervirulent [61].…”

Section: Indirect Gene Transfer Methods: Agrobacterium-mediated Transmentioning

confidence: 99%

“…Likewise, it is the most common method to obtain stably transformed grapevines [83,86,88,90,93]. The cocultivation of cell suspension cultures of Gamay Red with EHA105 strain of Agrobacterium was used for studying the expression of the grape dihydroflavonol 4-reductase gene (VviDFR) and the analysis of its promoter region [88].…”

Section: Cocultivationmentioning

confidence: 99%

“…The cocultivation of cell suspension cultures of Gamay Red with EHA105 strain of Agrobacterium was used for studying the expression of the grape dihydroflavonol 4-reductase gene (VviDFR) and the analysis of its promoter region [88]. In another work, Li et al [90] cocultivated somatic embryos of Thompson Seedless with EHA105 strain of Agrobacterium harboring a construct of VviMybA1 as a reporter gene and a vast number of grapevine constitutive promoters from various genotypes. Cocultivation has been the transformation method chosen for stable transformation of embryogenic and nonembryogenic cell cultures as well.…”

Section: Cocultivationmentioning

confidence: 99%

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Development and Use of Biotechnology Tools for Grape Functional Analysis

Chialva¹,

Eichler²,

Muñoz³

et al. 2016

Grape and Wine Biotechnology

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The aim of this chapter is to provide a description of the latest scientific advances in the field of gene functional analysis in grapevine. It provides general information about the studies conducted during the past decade to understand the natural variation of this plant and how this information has been exploited for the understanding of traits of interest. Likewise, it is exposed how the use of biotechnology tools have helped to characterize the mechanisms of gene expression and its regulation, as well as the subcellular localization of proteins and their interactions with other molecules. Finally, an approximation to the new technologies of gene editing and their potential application in the functional study of grapevine has been carried out.

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Somatic Embryogenesis and Genetic Modification of Vitis

Dhekney

Grant

et al. 2016

In Vitro Embryogenesis in Higher Plants

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Grapevine embryogenic cultures are ideal target tissues for inserting desired traits of interest and improving existing cultivars via precision breeding (PB). PB is a new approach that, like conventional breeding, utilizes only DNA fragments obtained from sexually compatible grapevine plants. Embryogenic culture induction occurs by placing leaves or stamens and pistils on induction medium with a dark/light photoperiod cycle for 12-16 weeks. Resulting cultures produce sectors of embryogenic and non-embryogenic callus, which can be identified on the basis of callus morphology and color. Somatic embryo development occurs following transfer of embryogenic callus to development medium and cultures can be maintained for extended periods of time by transfer of the proliferating proembryonic masses to fresh medium at 4-6-week intervals. To demonstrate plant recovery via PB, somatic embryos at the mid-cotyledonary stage are cocultivated with Agrobacterium containing the desired gene of interest along with a, non-PB, enhanced green fluorescent protein/neomycin phosphotransferase II (egfp/nptII) fusion gene. Modified cultures are grown on proliferation and development medium to produce uniformly modified somatic embryos via secondary embryogenesis. Modified embryos identified on the basis of green fluorescence and kanamycin resistance are transferred to germination medium for plant development. The resulting plants are considered to prototype examples of the PB approach, since they contain egfp/nptII, a non-grapevine-derived fusion gene. Uniform green fluorescent protein (GFP) fluorescence can be observed in all tissues of regenerated plants.

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Large-scale characterization of promoters from grapevine (Vitis spp.) using quantitative anthocyanin and GUS assay systems

Cited by 27 publications

References 50 publications

Promoters for Transgenic Horticultural Plants

Promoters for Transgenic Horticultural Plants

Development and Use of Biotechnology Tools for Grape Functional Analysis

Somatic Embryogenesis and Genetic Modification of Vitis

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