“…The enzyme activity of the adsorbed Tyr on ZnO nanorods was determined with the standard dopachrome method according to Fling et al [20]. These measurements explored a (153 ± 18) lmol of l-dopa oxidized per minute by Nafion/Tyr/ZnO film.…”
Section: Spectral Analysismentioning
confidence: 99%
“…The enzyme activity was estimated using the standard dopachrome method according to Fling et al [20]. The measurement was performed at 25°C by monitoring the absorption of dopachrome (e 475 nm = 3600 M À1 cm À1 ) at 475 nm after immersing the Tyr/ZnO/GCE electrode in a cuvette filled with 1 mL phosphate buffer (0.1 M, pH 6.8) containing 5 mM l-dopa for 30 s or 60 s. The activity was expressed in lmol of l-dopa oxidized per minute.…”
Section: Spectroscopic Analysis and Enzyme Activity Measurementsmentioning
“…The enzyme activity of the adsorbed Tyr on ZnO nanorods was determined with the standard dopachrome method according to Fling et al [20]. These measurements explored a (153 ± 18) lmol of l-dopa oxidized per minute by Nafion/Tyr/ZnO film.…”
Section: Spectral Analysismentioning
confidence: 99%
“…The enzyme activity was estimated using the standard dopachrome method according to Fling et al [20]. The measurement was performed at 25°C by monitoring the absorption of dopachrome (e 475 nm = 3600 M À1 cm À1 ) at 475 nm after immersing the Tyr/ZnO/GCE electrode in a cuvette filled with 1 mL phosphate buffer (0.1 M, pH 6.8) containing 5 mM l-dopa for 30 s or 60 s. The activity was expressed in lmol of l-dopa oxidized per minute.…”
Section: Spectroscopic Analysis and Enzyme Activity Measurementsmentioning
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