Large-Scale Expansion of Porcine Adipose-Derived Stem Cells Based on Microcarriers System for Cultured Meat Production

Song, Wenjuan; Li, Peipei; Li, Huixia; Ding, Shijie

doi:10.3390/foods11213364

Cited by 12 publications

(9 citation statements)

References 37 publications

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“…Previous studies have shown that many primary preadipocytes can grow on Cytodex 1 microcarriers 18,20 . However, the process of digesting cells from microcarriers is required since Cytodex1 is inedible, increasing workloads and costs.…”

Section: Resultsmentioning

confidence: 99%

“…For large-scale expansion of seed cells for cell-cultured meat, acclimating to 3D-scale culture is critical. Several primary preadipocyte cell lines have been reported to be amplified on microcarriers in suspension bioreactors to increase scalability 18,20 . We reported that ISP-4 could achieve large-scale expansion on microcarriers at the density of 18 × 10 5 cells per ml.…”

Section: Discussionmentioning

confidence: 99%

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An immortal porcine preadipocyte cell strain for efficient production of cell-cultured fat

Cheng,

Hong,

Song

et al. 2023

Commun Biol

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Adding adipose cells to cell-cultured meat can provide a distinctive aroma and juicy texture similar to real meat. However, a significant challenge still exists in obtaining seed cells that can be propagated for long periods, maintain their adipogenic potential, and reduce production costs. In this study, we present a cell strain derived from immortalized porcine preadipocytes that can be subculture for over 40 passages without losing differentiation capacity. This cell strain can be differentiated within 3D bioscaffolds to generate cell-cultured fat using fewer chemicals and less serum. Additionally, it can be expanded and differentiated on microcarriers with upscaled culture to reduce costs and labor. Moreover, it can co-differentiate with muscle precursor cells, producing a pattern similar to real meat. Therefore, our cell strain provides an exceptional model for studying and producing cell-cultured fat.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

An immortal porcine preadipocyte cell strain for efficient production of cell-cultured fat

Cheng,

Hong,

Song

et al. 2023

Commun Biol

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show abstract

“…For large-scale expansion of seed cells for cell-cultured meat, acclimating to 3D-scale culture is critical. Several primary preadipose cell lines have been reported to be ampli ed on microcarriers in suspension bioreactors to increase scalability 18,20 . We reported that Pig#4 could achieve large-scale expansion on microcarriers at the density of 18 x 10 5 cells per ml.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, further work should focus on adapting Pig#4 to anchorage-independent growth. Moreover, the primary preadipocytes need to be harvested from microcarriers before adipogenic differentiation 18,20 . Although it remains unclear whether it is because the microcarriers are inedible, or these primary cells cannot be differentiated on the microcarriers, the inadequacy of these protocols is apparent, which increases the labor costs.…”

Section: Discussionmentioning

confidence: 99%

An immortal porcine preadipose cell strain for efficient production of cell-cultured fat

Huang

Cheng

Hong

et al. 2023

Preprint

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Adding adipose cells to cell-cultured meat can provide a distinctive aroma and juicy texture similar to real meat. However, a significant challenge still exists in obtaining seed cells that can be propagated for long periods, maintain their adipogenic potential, and reduce production costs. In this study, we present a cell strain derived from immortalized porcine preadipocytes that can be subculture for over 40 passages without losing differentiation capacity. This cell strain can be differentiated within 3D bioscaffolds to generate cell-cultured fat using fewer chemicals and less serum. Additionally, it can be expanded and differentiated on microcarriers with upscaled culture to reduce costs and labor. Moreover, it can co-differentiate with muscle precursor cells, producing a marbling pattern. Therefore, our cell strain provides an exceptional model for studying and producing cell-cultured fat.

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“…Many cell sources have been explored, including muscle satellite cells, adipose-derived stem cells, multipotent mesenchymal stem cells, induced pluripotent stem cells, transgenics, and immortalized cells [2]. Among them, multipotent mesenchymal stem cells (stem cells) stand out as promising candidates due to their abilities of self-renewal, differentiation, and their relatively easy acquirement from multiple types of tissues [1,11]. Stem cells or progenitor cells used in cultivated meat in current literature are mostly isolated from tissue biopsies via enzymatic digestion.…”

Section: Introductionmentioning

confidence: 99%

Bovine Fibroblast-Derived Extracellular Matrix Promotes the Growth and Preserves the Stemness of Bovine Stromal Cells during In Vitro Expansion

Lee

Jackson

John

et al. 2023

JFB

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Cultivated meat is a fast-growing research field and an industry with great potential to overcome the limitations of traditional meat production. Cultivated meat utilizes cell culture and tissue engineering technologies to culture a vast number of cells in vitro and grow/assemble them into structures mimicking the muscle tissues of livestock animals. Stem cells with self-renewal and lineage-specific differentiation abilities have been considered one of the key cell sources for cultivated meats. However, the extensive in vitro culturing/expansion of stem cells results in a reduction in their abilities to proliferate and differentiate. Extracellular matrix (ECM) has been used as a culturing substrate to support cell expansion for cell-based therapies in regenerative medicine due to its resemblance to the native microenvironment of cells. In this study, the effect of the ECM on the expansion of bovine umbilical cord stromal cells (BUSC) in vitro was evaluated and characterized. BUSCs with multi-lineage differentiation potentials were isolated from bovine placental tissue. Decellularized ECM prepared from a confluent monolayer of bovine fibroblasts (BF) is free of cellular components but contains major ECM proteins such as fibronectin and type I collagen and ECM-associated growth factors. Expansion of BUSC on ECM for three passages (around three weeks) resulted in about 500-fold amplification, while cells were amplified less than 10-fold when cultured on standard tissue culture plates (TCP). Moreover, the presence of ECM reduced the requirement for serum in the culture medium. Importantly, the cells amplified on ECM retained their differentiation abilities better than cells cultured on TCP. The results of our study support the notion that monolayer cell-derived ECM may be a strategy to expand bovine cells in vitro effectively and efficiently.

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Large-Scale Expansion of Porcine Adipose-Derived Stem Cells Based on Microcarriers System for Cultured Meat Production

Cited by 12 publications

References 37 publications

An immortal porcine preadipocyte cell strain for efficient production of cell-cultured fat

An immortal porcine preadipocyte cell strain for efficient production of cell-cultured fat

An immortal porcine preadipose cell strain for efficient production of cell-cultured fat

Bovine Fibroblast-Derived Extracellular Matrix Promotes the Growth and Preserves the Stemness of Bovine Stromal Cells during In Vitro Expansion

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